scholarly journals Cell migration and organization in three-dimensional in vitro culture driven by stiffness gradient

2016 ◽  
Vol 113 (11) ◽  
pp. 2496-2506 ◽  
Author(s):  
Danielle Joaquin ◽  
Michael Grigola ◽  
Gubeum Kwon ◽  
Christopher Blasius ◽  
Yutao Han ◽  
...  
Keyword(s):  
Cell Migration ◽  
In Vitro Culture ◽  
Three Dimensional

scholarly journals Beyond 3D culture models of cancer

2015 ◽  
Vol 7 (283) ◽  
pp. 283ps9-283ps9 ◽  
Author(s):  
Kandice Tanner ◽  
Michael M. Gottesman
Keyword(s):  
In Vitro Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Drug Efficacy ◽  
Spatiotemporal Evolution ◽  
Culture Models ◽  
Dynamic Interplay

The mechanisms underlying the spatiotemporal evolution of tumor ecosystems present a challenge in evaluating drug efficacy. In this Perspective, we address the use of three-dimensional in vitro culture models to delineate the dynamic interplay between the tumor and the host microenvironment in an effort to attain realistic platforms for assessing pharmaceutical efficacy in patients.

scholarly journals In vitro culture of hFOB1.19 osteoblast cells on TGF-β1-SF-CS three-dimensional scaffolds

2015 ◽  
Vol 13 (1) ◽  
pp. 181-187 ◽  
Author(s):  
SHUANG TONG ◽  
LEI XUE ◽  
DA-PENG XU ◽  
ZI-MEI LIU ◽  
YANG DU ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Three Dimensional ◽  
Osteoblast Cells ◽  
Tgf Β1

scholarly journals Modeling Liver Organogenesis by Recreating Three-Dimensional Collective Cell Migration: A Role for TGFβ Pathway

2021 ◽  
Vol 9 ◽  
Author(s):  
Ogechi Ogoke ◽  
Osama Yousef ◽  
Cortney Ott ◽  
Allison Kalinousky ◽  
Wayne Lin ◽  
...  
Keyword(s):  
Cell Migration ◽  
Liver Cell ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Three Dimensional ◽  
3D Models ◽  
Branching Morphogenesis ◽  
Lung Fibroblasts ◽  
Collective Cell Migration

Three-dimensional (3D) collective cell migration (CCM) is critical for improving liver cell therapies, eliciting mechanisms of liver disease, and modeling human liver development and organogenesis. Mechanisms of CCM differ in 2D vs. 3D systems, and existing models are limited to 2D or transwell-based systems, suggesting there is a need for improved 3D models of CCM. To recreate liver 3D CCM, we engineered in vitro 3D models based upon a morphogenetic transition that occurs during liver organogenesis, which occurs rapidly between E8.5 and E9.5 (mouse). During this morphogenetic transition, 3D CCM exhibits co-migration (multiple cell types), thick-strand interactions with surrounding septum transversum mesenchyme (STM), branching morphogenesis, and 3D interstitial migration. Here, we engineer several 3D in vitro culture systems, each of which mimics one of these processes in vitro. In mixed spheroids bearing both liver cells and uniquely MRC-5 (fetal lung) fibroblasts, we observed evidence of co-migration, and a significant increase in length and number of liver spheroid protrusions, which was highly sensitive to transforming growth factor beta 1 (TGFβ1) stimulation. In MRC-5-conditioned medium (M-CM) experiments, we observed dose-dependent branching morphogenesis associated with an upregulation of Twist1, which was inhibited by a broad TGFβ inhibitor. In models in which liver spheroids and MRC-5 spheroids were co-cultured, we observed complex strand morphogenesis, whereby thin, linear, 3D liver cell strands attach to the MRC-5 spheroid, anchor and thicken to form permanent and thick anchoring contacts between the two spheroids. In these spheroid co-culture models, we also observed spheroid fusion and strong evidence for interstitial migration. In conclusion, we present several novel cultivation systems that recreate distinct features of liver 3D CCM. These methodologies will greatly improve our molecular, cellular, and tissue-scale understanding of liver organogenesis, liver diseases like cancer, and liver cell therapy, and will also serve as a tool to bridge conventional 2D studies and preclinical in vivo studies.

scholarly journals Development of Spermatogenesis In Vitro in Three-Dimensional Culture from Spermatogonial Cells of Busulfan-Treated Immature Mice

2018 ◽  
Vol 19 (12) ◽  
pp. 3804 ◽  
Author(s):  
Ali AbuMadighem ◽  
Ronnie Solomon ◽  
Alina Stepanovsky ◽  
Joseph Kapelushnik ◽  
QingHua Shi ◽  
...  
Keyword(s):  
Male Infertility ◽  
In Vitro Culture ◽  
Seminiferous Tubule ◽  
Three Dimensional ◽  
Testicular Tissue ◽  
Biologically Active ◽  
Isolated Cells ◽  
Dead Box ◽  
Testicular Weight

Aggressive chemotherapy may lead to permanent male infertility. Prepubertal males do not generate sperm, but their testes do contain spermatogonial cells (SPGCs) that could be used for fertility preservation. In the present study, we examined the effect of busulfan (BU) on the SPGCs of immature mice, and the possible induction of the survivor SPGCs to develop spermatogenesis in 3D in-vitro culture. Immature mice were injected with BU, and after 0.5–12 weeks, their testes were weighed and evaluated histologically compared to the control mice. The spermatogonial cells [Sal-like protein 4 (SALL4) and VASA (a member of the DEAD box protein family) in the testicular tissue were counted/seminiferous tubule (ST). The cells from the STs were enzymatically isolated and cultured in vitro. Our results showed a significant decrease in the testicular weight of the BU-treated mice compared to the control. This was in parallel to a significant increase in the number of severely damaged STs, and a decrease in the number of SALL4 and VASA/STs compared to the control. The cultures of the isolated cells from the STs of the BU-treated mice showed a development of colonies and meiotic and post-meiotic cells after four weeks of culture. The addition of homogenates from adult GFP mice to those cultures induced the development of sperm-like cells after four weeks of culture. This is the first study demonstrating the presence of biologically active spermatogonial cells in the testicular tissue of BU-treated immature mice, and their capacity to develop sperm-like cells in vitro.

Three dimensional in vitro culture of preantral follicles following slow-freezing and vitrification of mouse ovarian tissue

Cryobiology ◽  
2015 ◽  
Vol 71 (3) ◽  
pp. 529-536 ◽  
Author(s):  
Fatemeh Asgari ◽  
Mojtaba Rezazadeh Valojerdi ◽  
Bita Ebrahimi ◽  
Roya Fatehi
Keyword(s):  
In Vitro Culture ◽  
Ovarian Tissue ◽  
Three Dimensional ◽  
Slow Freezing ◽  
Preantral Follicles
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