scholarly journals Platelet-Derived Growth Factor and Spatiotemporal Cues Induce Development of Vascularized Bone Tissue by Adipose-Derived Stem Cells

2013 ◽  
Vol 19 (17-18) ◽  
pp. 2076-2086 ◽  
Author(s):  
Daphne L. Hutton ◽  
Erika M. Moore ◽  
Jeffrey M. Gimble ◽  
Warren L. Grayson
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Bone Tissue ◽  
Platelet Derived Growth Factor ◽  
Adipose Derived Stem Cells ◽  
Vascularized Bone

Platelet-derived growth-factor-releasing aligned collagen–nanoparticle fibers promote the proliferation and tenogenic differentiation of adipose-derived stem cells

2014 ◽  
Vol 10 (3) ◽  
pp. 1360-1369 ◽  
Author(s):  
XingGuo Cheng ◽  
Christopher Tsao ◽  
Victor L. Sylvia ◽  
Douglas Cornet ◽  
Daniel P. Nicolella ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Platelet Derived Growth Factor ◽  
Adipose Derived Stem Cells ◽  
Tenogenic Differentiation

Tendon Transection Healing Can Be Improved With Adipose-Derived Stem Cells Cultured With Growth Differentiation Factor 5 and Platelet-Derived Growth Factor

Hand ◽  
2021 ◽  
pp. 155894472110289
Author(s):  
Michael J. Fitzgerald ◽  
Taylor Mustapich ◽  
Haixiang Liang ◽  
Christopher G. Larsen ◽  
Kate W. Nellans ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Cellular Proliferation ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Platelet Derived Growth Factor ◽  
Adipose Derived Stem Cells ◽  
Differentiation Factor ◽  
Growth Differentiation Factor 5 ◽  
Cells Cultured

Background: As hand surgeons, tendon injuries and lacerations are a particularly difficult problem to treat, as poor healing potential and adhesions hamper optimal recovery. Adipose-derived stem cells (ADSCs) have been shown to aid in rat Achilles tendon healing after a puncture defect, and this model can be used to study tendon healing in the upper extremity. We hypothesized that ADSCs cultured with growth differentiation factor 5 (GDF5) and platelet-derived growth factor (PDGF) would improve tendon healing after a transection injury. Methods: Rat Achilles tendons were transected and then left either unrepaired or repaired. Both groups were treated with a hydrogel alone, a hydrogel with ADSCs, or a hydrogel with ADSCs that were cultured with GDF5 and PDGF prior to implantation. Tissue harvested from the tendons was evaluated for gene expression of several genes known to play an important role in successful tendon healing. Histological examination of the tendon healing was also performed. Results: In both repaired and unrepaired tendons, those treated with ADSCs cultured with GDF5/PDGF prior to implantation showed the best tendon fiber organization, the smallest gaps, and the most organized blood vessels. Treatment with GDF5/PDGF increased expression of the protenogenesis gene SOX9, promoted cell-to-cell connections, improved cellular proliferation, and enhanced tissue remodeling. Conclusions: Adipose-derived stem cells cultured with GDF5/PDGF prior to implantation can promote tendon repair by improving cellular proliferation, tenogenesis, and vascular infiltration. This effect results in a greater degree of organized tendon healing.

Developmentally regulated use of alternative promoters creates a novel platelet-derived growth factor receptor transcript in mouse teratocarcinoma and embryonic stem cells

1989 ◽  
Vol 9 (10) ◽  
pp. 4563-4567
Author(s):  
T H Vu ◽  
G R Martin ◽  
P Lee ◽  
D Mark ◽  
A Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Growth Factor ◽  
Short Form ◽  
Embryonic Stem ◽  
Growth Factor Receptor ◽  
Extracellular Domain ◽  
Platelet Derived Growth Factor ◽  
Alternative Promoters

Embryonal carcinoma and embryonic stem cells expressed a novel form of platelet-derived growth factor receptor mRNA which was approximately 1,100 base pairs shorter than the 5.3-kilobase (kb) transcript expressed in fibroblasts and other cell types. The 4.2-kb stem cell transcript was initiated within the genomic region immediately upstream of exon 6 of the 5.3-kb transcript and therefore lacked the first five exons, which encode much of the extracellular domain of the receptor expressed in fibroblasts. In stem cells, the short form was predominant, although both forms were present at low levels. Following differentiation in vitro, expression levels of the long form increased dramatically. These findings suggest that during early embryogenesis, a stem cell-specific promoter is used in a stage- and cell type-specific manner to express a form of the platelet-derived growth factor receptor that lacks much of the extracellular domain and may function independently of ligand.

scholarly journals Use of adipose-derived stem cells in lymphatic tissue engineering and regeneration

2021 ◽  
Vol 48 (5) ◽  
pp. 559-567
Author(s):  
Antonio Jorge Forte ◽  
Daniel Boczar ◽  
Rachel Sarabia-Estrada ◽  
Maria T. Huayllani ◽  
Francisco R. Avila ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Systematic Review ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Factor ◽  
Cochrane Central Register ◽  
Adipose Derived Stem Cells ◽  
Lymphatic Tissue ◽  
Factor C

The potential to differentiate into different cell lines, added to the easy and cost-effective method of extraction, makes adipose-derived stem cells (ADSCs) an object of interest in lymphedema treatment. Our study’s goal was to conduct a comprehensive systematic review of the use of ADSCs in lymphatic tissue engineering and regeneration. On July 23, 2019, using PubMed/MEDLINE, Cochrane Clinical Answers, Cochrane Central Register of Controlled Trials, and Embase databases, we conducted a systematic review of published literature on the use of ADSCs in lymphatic tissue engineering and regeneration. There were no language or time frame limitations, and the following search strategy was applied: ((Adipose stem cell) OR Adipose-derived stem cell)) AND ((Lymphedema) OR Breast Cancer Lymphedema). Only original research manuscripts were included. Fourteen studies fulfilled the inclusion criteria. Eleven studies were experimental (in vitro or in vivo in animals), and only three were clinical. Publications on the topic demonstrated that ADSCs promote lymphangiogenesis, and its effect could be enhanced by modulation of vascular endothelial growth factor-C, interleukin-7, prospero homeobox protein 1, and transforming growth factor-β1. Pilot clinical studies included 11 patients with breast cancer-related lymphedema, and no significant side effects were present at 12-month follow-up. Literature on the use of ADSCs in lymphatic tissue engineering and regeneration demonstrated promising data. Clinical evidence is still in its infancy, but the scientific community agrees that ADSCs can be useful in regenerative lymphangiogenesis. Data collected in this review indicate that unprecedented advances in lymphedema treatment can be anticipated in the upcoming years.

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