Dextran‐based hydrogel with enhanced mechanical performance via covalent and non‐covalent cross‐linking units carrying adipose‐derived stem cells toward vascularized bone tissue engineering

2019 ◽  
Vol 107 (6) ◽  
pp. 1120-1131 ◽  
Author(s):  
Litao Cai ◽  
Jitian Li ◽  
Songtao Quan ◽  
Wei Feng ◽  
Junna Yao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Performance ◽  
Cross Linking ◽  
Adipose Derived Stem Cells ◽  
Vascularized Bone

scholarly journals Microengineered platforms for co-cultured mesenchymal stem cells towards vascularized bone tissue engineering

2016 ◽  
Vol 13 (5) ◽  
pp. 465-474 ◽  
Author(s):  
Hyeryeon Park ◽  
Dong-Jin Lim ◽  
Minhee Sung ◽  
Soo-Hong Lee ◽  
Dokyun Na ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Vascularized Bone

scholarly journals Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

2014 ◽  
Vol 356 (1) ◽  
pp. 97-107 ◽  
Author(s):  
Wei Lu ◽  
Kun Ji ◽  
Jennifer Kirkham ◽  
Yu Yan ◽  
Aldo R. Boccaccini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Adipose Derived Stem Cells

Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold

2008 ◽  
Vol 84A (1) ◽  
pp. 191-197 ◽  
Author(s):  
Natsuko Kakudo ◽  
Ayuko Shimotsuma ◽  
Shogo Miyake ◽  
Satoshi Kushida ◽  
Kenji Kusumoto
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Collagen Scaffold ◽  
Adipose Derived Stem Cells

Osteogenic differentiation of adipose-derived stem cells in mesoporous SBA-16 and SBA-16 hydroxyapatite scaffolds

RSC Advances ◽  
2015 ◽  
Vol 5 (67) ◽  
pp. 54551-54562 ◽  
Author(s):  
Gracielle F. Andrade ◽  
Juliana L. Carvalho ◽  
Armando S. C. Júnior ◽  
Alfredo M. Goes ◽  
Edésia M. B. Sousa
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Adipose Derived Stem Cells ◽  
Engineering Applications

Adipose-derived stem cells (ASCs) are currently a point of focus for bone tissue engineering applications.

scholarly journals Composite Hydrogel of Methacrylated Hyaluronic Acid and Fragmented Polycaprolactone Nanofiber for Osteogenic Differentiation of Adipose-Derived Stem Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 902
Author(s):  
Madhumita Patel ◽  
Won-Gun Koh
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Hyaluronic Acid ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Composite Hydrogel ◽  
Adipose Derived Stem Cells ◽  
Alizarin Red ◽  
Composite Hydrogels

Composite hydrogels with electrospun nanofibers (NFs) have recently been used to mimic the native extracellular matrix. In this study, composite hydrogels of methacrylated hyaluronic acid containing fragmented polycaprolactone NFs were used for bone tissue engineering. The composite (NF/hydrogel) was crosslinked under ultraviolet (UV) light. The incorporation of fragmented polycaprolactone NFs increased the compression modulus from 1762.5 to 3122.5 Pa. Subsequently, adipose-derived stem cells incorporated into the composite hydrogel exhibited a more stretched and elongated morphology and osteogenic differentiation in the absence of external factors. The mRNA expressions of osteogenic biomarkers, including collagen 1 (Col1), alkaline phosphatase, and runt-related transcription factor 2, were 3–5-fold higher in the composite hydrogel than in the hydrogel alone. In addition, results of the protein expression of Col1 and alizarin red staining confirmed osteogenic differentiation. These findings suggest that our composite hydrogel provides a suitable microenvironment for bone tissue engineering.

scholarly journals Low level laser therapy promotes bone regeneration by coupling angiogenesis and osteogenesis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jie Bai ◽  
Lijun Li ◽  
Ni Kou ◽  
Yuwen Bai ◽  
Yaoyang Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Low Level Laser Therapy ◽  
Level Laser ◽  
Vascularized Bone

Abstract Background Bone tissue engineering is a new concept bringing hope for the repair of large bone defects, which remains a major clinical challenge. The formation of vascularized bone is key for bone tissue engineering. Growth of specialized blood vessels termed type H is associated with bone formation. In vivo and in vitro studies have shown that low level laser therapy (LLLT) promotes angiogenesis, fracture healing, and osteogenic differentiation of stem cells by increasing reactive oxygen species (ROS). However, whether LLLT can couple angiogenesis and osteogenesis, and the underlying mechanisms during bone formation, remains largely unknown. Methods Mouse bone marrow mesenchymal stem cells (BMSCs) combined with biphasic calcium phosphate (BCP) grafts were implanted into C57BL/6 mice to evaluate the effects of LLLT on the specialized vessel subtypes and bone regeneration in vivo. Furthermore, human BMSCs and human umbilical vein endothelial cells (HUVECs) were co-cultured in vitro. The effects of LLLT on cell proliferation, angiogenesis, and osteogenesis were assessed. Results LLLT promoted the formation of blood vessels, collagen fibers, and bone tissue and also increased CD31hiEMCNhi-expressing type H vessels in mBMSC/BCP grafts implanted in mice. LLLT significantly increased both osteogenesis and angiogenesis, as well as related gene expression (HIF-1α, VEGF, TGF-β) of grafts in vivo and of co-cultured BMSCs/HUVECs in vitro. An increase or decrease of ROS induced by H2O2 or Vitamin C, respectively, resulted in an increase or decrease of HIF-1α, and a subsequent increase and decrease of VEGF and TGF-β in the co-culture system. The ROS accumulation induced by LLLT in the co-culture system was significantly decreased when HIF-1α was inhibited with DMBPA and was followed by decreased expression of VEGF and TGF-β. Conclusions LLLT enhanced vascularized bone regeneration by coupling angiogenesis and osteogenesis. ROS/HIF-1α was necessary for these effects of LLLT. LLLT triggered a ROS-dependent increase of HIF-1α, VEGF, and TGF-β and resulted in subsequent formation of type H vessels and osteogenic differentiation of mesenchymal stem cells. As ROS also was a target of HIF-1α, there may be a positive feedback loop between ROS and HIF-1α, which further amplified HIF-1α induction via the LLLT-mediated ROS increase. This study provided new insight into the effects of LLLT on vascularization and bone regeneration in bone tissue engineering.

scholarly journals Human adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blend

2017 ◽  
Vol 2 (2) ◽  
pp. 71-81 ◽  
Author(s):  
Esen Sayin ◽  
Rosti Hama Rashid ◽  
José Carlos Rodríguez-Cabello ◽  
Ahmed Elsheikh ◽  
Erkan Türker Baran ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Adipose Derived Stem Cells ◽  
Human Osteoblasts
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