scholarly journals Proteomic Profiling of Tissue-Engineered Blood Vessel Walls Constructed by Adipose-Derived Stem Cells

2013 ◽  
Vol 19 (3-4) ◽  
pp. 415-425 ◽  
Author(s):  
Chen Wang ◽  
Fangfang Guo ◽  
Heng Zhou ◽  
Yun Zhang ◽  
Zhigang Xiao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Vessel ◽  
Adipose Derived Stem Cells ◽  
Proteomic Profiling ◽  
Vessel Walls

scholarly journals Quantitative Proteomic Profiling of Small Molecule Treated Mesenchymal Stem Cells Using Chemical Probes

2020 ◽  
Vol 22 (1) ◽  
pp. 160
Author(s):  
Jerran Santos ◽  
Sibasish Dolai ◽  
Matthew B. O’Rourke ◽  
Fei Liu ◽  
Matthew P. Padula ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ribosomal Proteins ◽  
Surface Protein ◽  
Temporal Analysis ◽  
Phenotypic Characterization ◽  
Major Protein ◽  
Adipose Derived Stem Cells ◽  
Protein Markers ◽  
Proteomic Profiling ◽  
Depth Analysis

The differentiation of human adipose derived stem cells toward a neural phenotype by small molecules has been a vogue topic in the last decade. The characterization of the produced cells has been explored on a broad scale, examining morphological and specific surface protein markers; however, the lack of insight into the expression of functional proteins and their interactive partners is required to further understand the extent of the process. The phenotypic characterization by proteomic profiling allows for a substantial in-depth analysis of the molecular machinery induced and directing the cellular changes through the process. Herein we describe the temporal analysis and quantitative profiling of neural differentiating human adipose-derived stem cells after sub-proteome enrichment using a bisindolylmaleimide chemical probe. The results show that proteins enriched by the Bis-probe were identified reproducibly with 133, 118, 126 and 89 proteins identified at timepoints 0, 1, 6 and 12, respectively. Each temporal timepoint presented several shared and unique proteins relative to neural differentiation and their interactivity. The major protein classes enriched and quantified were enzymes, structural and ribosomal proteins that are integral to differentiation pathways. There were 42 uniquely identified enzymes identified in the cells, many acting as hubs in the networks with several interactions across the network modulating key biological pathways. From the cohort, it was found by gene ontology analysis that 18 enzymes had direct involvement with neurogenic differentiation.

Abstract 360: Development of a Human Tissue-Engineered Blood Vessel from Adipose-Derived Stem Cells

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Jaclyn A Brennan ◽  
Julien H Arrizabalaga ◽  
Matthias U Nollert
Keyword(s):  
Stem Cells ◽  
Blood Vessel ◽  
Amniotic Membrane ◽  
Biological Material ◽  
Small Diameter ◽  
Human Amniotic Membrane ◽  
Adipose Derived Stem Cells ◽  
Cell Nuclei ◽  
Static Culture ◽  
Biological Substrate

There exists a clear need for alternative sources of small-diameter vascular grafts for treating the millions of patients who suffer from cardiovascular disease each year. Bypass surgery or replacement of defective vessels is often required to treat coronary heart disease, but there is a limited supply of suitable autologous grafts, and synthetic grafts are ineffective for replacement of small-diameter vessels. Inherent thrombogenicity, compliance mismatch, and limited patency rates are all complications with current options. Tissue engineering has the potential to overcome these limitations by producing a readily-available vascular graft completely from biological material. It is the objective of this study to fabricate such a small-diameter tissue engineered blood vessel (TEBV) by using the human amniotic membrane as a mechanically-sound biological substrate. Our technology begins by differentiating adipose-derived stem cells into smooth muscle cells (SMCs) and seeding them onto a flat sheet of the amniotic membrane. We assessed our hypothesis that several types of SMCs can successfully attach and proliferate on this membrane by fluorescently staining cell nuclei with DAPI and characteristic SMC actin filaments with phallotoxins. After 7 days in static culture, the cell-seeded sheet was wrapped around a 3mm O.D. removable mandrel with 6-7 revolutions to develop a tubular construct with architecture akin to that of a muscular artery’s tunica media layer. After a 2 week static culture period, the TEBV was characterized for its biochemical and mechanical properties. We examined the contraction of the vessel in response to carbachol, a specific agonist for SMCs, and compared our results with the contraction of porcine coronary arteries. Burst pressure and elastic modulus tests were also performed. The mechanical integrity of this construct can be further improved upon its exposure to appropriate physiological conditions in a perfusion bioreactor. We show that adipose-derived endothelial cells (ECs) can be also be seeded into the lumen of this construct to prevent platelet adhesion. In conclusion, we have developed a small-diameter TEBV with off-the-shelf availability using a completely biological material seeded with patient-own stem cells.

scholarly journals Adipose-Derived Stem Cells Versus Artery Explants as Sources of Autologous Smooth Muscle for Blood Vessel Bioengineering

2011 ◽  
Vol 54 (6) ◽  
pp. 1862-1863
Author(s):  
Brian W. Bernish ◽  
Xue Ma ◽  
Zhan Wang ◽  
Masood Machingal ◽  
Shay Soker ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Blood Vessel ◽  
Adipose Derived Stem Cells

Osteoplasty in Disseminated Calvarial Defects Treated with Autologous Adipose-Derived Stem Cells and Autologous Fibrin Glue: Case Report

Skull Base ◽  
2005 ◽  
Vol 15 (S 2) ◽  
Author(s):  
Stefan Lendeckel ◽  
A. Jödicke ◽  
P. Christophis ◽  
K. Heidinger ◽  
H.-P. Howaldt
Keyword(s):  
Stem Cells ◽  
Case Report ◽  
Fibrin Glue ◽  
Adipose Derived Stem Cells ◽  
Autologous Fibrin Glue ◽  
Calvarial Defects
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