scholarly journals Adipose-Derived Stem Cells Versus Artery Explants as Sources of Autologous Smooth Muscle for Blood Vessel Bioengineering

2011 ◽  
Vol 54 (6) ◽  
pp. 1862-1863
Author(s):  
Brian W. Bernish ◽  
Xue Ma ◽  
Zhan Wang ◽  
Masood Machingal ◽  
Shay Soker ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Blood Vessel ◽  
Adipose Derived Stem Cells

scholarly journals MicroRNA-145 regulates the differentiation of human adipose-derived stem cells to smooth muscle cells via targeting Krüppel-like factor 4

2017 ◽  
Vol 15 (6) ◽  
pp. 3787-3795 ◽  
Author(s):  
Kaisaier Aji ◽  
Yun Zhang ◽  
Abudusaimi Aimaiti ◽  
Yujie Wang ◽  
Mulati Rexiati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Adipose Derived Stem Cells

Stem Cells and Vascular Regenerative Medicine

2008 ◽  
Author(s):  
Taby Ahsan ◽  
Adele M. Doyle ◽  
Garry P. Duffy ◽  
Frank Barry ◽  
Robert M. Nerem
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Smooth Muscle ◽  
Regenerative Medicine ◽  
Blood Vessel ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
In Vitro Proliferation ◽  
The Impact ◽  
Blood Vessel Substitutes

Vascular applications in regenerative medicine include blood vessel substitutes and vasculogenesis in ischemic or engineered tissues. For these repair processes to be successful, there is a need for a stable supply of endothelial and smooth muscle cells. For blood vessel substitutes, the immediate goal is to enable blood flow, but vasoactivity is necessary for long term success. In engineered vessels, it is thought that endothelial cells will serve as an anti-thrombogenic lumenal layer, while smooth muscle cells contribute to vessel contractility. In other clinical applications, what is needed is not a vessel substitute but the promotion of new vessel formation (vasculogenesis). A simplified account of vasculogenesis is that endothelial cells assemble to form vessel-like structures that can then be stabilized by smooth muscle cells. Overall, the need for new vasculature to transfer oxygen and nutrients is important to reperfuse not only ischemic tissue in vivo, but also dense, structurally complex engineered tissue. The impact of these vascular therapies, however, is limited in part by the low yield and inadequate in vitro proliferation potential of primary endothelial and smooth muscle cells. Thus, there is a need to address the cell sourcing issue for vascular cell-based therapies, potentially using stem cells.

scholarly journals Transplantation of adipose derived stem cells overexpressing inducible nitric oxide synthase ameliorates diabetes mellitus induced erectile dysfunction in rats

2019 ◽  
Author(s):  
Yan Zhang ◽  
Jun Yang ◽  
Li Zhuan ◽  
Guanghui Zang ◽  
Tao Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Diabetes Mellitus ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Erectile Dysfunction ◽  
Inducible Nitric Oxide Synthase ◽  
Collagen Iv ◽  
Adipose Derived Stem Cells ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Background: Erectile dysfunction is a major complication of diabetes mellitus. Adipose derived stem cells (ADSCs) has attracted much attention as a promising tool for the treatment of diabetes mellitus induced erectile dysfunction (DMED). Inducible nitric oxide synthase (iNOS) plays an important role in protecting penile tissues from fibrosis. The aim of this study was to determine the efficacy of ADSCs overexpressing iNOS on DMED in rats. Methods: ADSCs were isolated and infected with adenovirus overexpressing iNOS (named as ADSCs-iNOS). The expression of iNOS was detected using western blot analysis and real-time PCR. Rats were randomly assigned into five groups: control group, DMED group, ADSCs group, ADSCs-EGFP group and ADSCs-iNOS group. 5×105 cells were given once via the intracorporal route. Two weeks after treatment, erectile function was assessed by electrical stimulation of the cavernous nerve. Penile tissues were obtained and evaluated at histology level. Results: We found that ADSCs-iNOS had significantly higher expression of iNOS at mRNA and protein levels and generated more nitric oxide. ADSCs-iNOS reduced collagen I and collagen IV expression of corpus cavernosum smooth muscle cells (CCSMCs) in cell co-culture model. Transforming growth factor-β1 expression and p-Smad2/3 to Smad2/3 ratio in CCSMCs reduced following co-culture with ADSCs-iNOS. Injection of ADSCs-iNOS significantly ameliorated DMED in rats and decreased collagen/smooth muscle cell ratio of penile tissues. Moreover, elevated nitric oxide and cGMP concentrations were detected in penile tissues of ADSCs-iNOS group. Conclusion: Taken together, ADSCs-iNOS significantly improve erectile function of DMED rats. The therapeutic effect may be achieved by increased nitric oxide generation and the suppression of collagen I and collagen IV expression in the CCSMCs to decrease penile fibrosis.

scholarly journals TGF-β1 Regulates Smooth Muscle Cells Differentiation from Adipose-Derived Stem Cells via the Wnt/β-Catenin Pathway

2021 ◽  
Author(s):  
Xuling Lv ◽  
Hao Chen ◽  
Zikai Zhang ◽  
Tian Li ◽  
Qing Wei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Pelvic Floor ◽  
Signaling Pathway ◽  
Myogenic Differentiation ◽  
Pelvic Floor Dysfunction ◽  
Muscle Cells ◽  
Adipose Derived Stem Cells ◽  
Tgf Β1

Abstract Background: Pelvic floor dysfunction (PFD) is a spectrum of disorders including stress urinary incontinence and pelvic organ prolapse. Transforming growth factor-β1 (TGF-β1) can induce mesenchymal stem cells (MSCs) to differentiate into smooth muscle cells (SMCs). SMCs derived from adipose-derived stem cells (ADSCs) can be used to repair damaged pelvic floor smooth muscle tissues, which is of great interest for clinical applications using stem cell therapy for PFD. The Wnt/β-catenin pathway acts as a decisive factor in the fate of stem cells.Methods and Results: In this study, we used medium containing TGF-β1, TGF-β1 inhibitor LY2109761, or Wnt/β-catenin inhibitor KYA1797K, to induce ADCSs to differentiate into SMCs in vitro to explore the influence of TGF-β1 on the myogenic differentiation of ADCSs via the Wnt/β-catenin pathway. Results: 1) TGF-β1 induces ADSC-derived SMCs to hyper-express the SMC markers including SMA-α, Desmin, Calponin, and SMMHC ; 2) TGF-β1 activates the Wnt/β-catenin signaling pathway in ADSCs. After blocking TGF-β1, the Wnt/β-catenin pathway and myogenic differentiation in cells were inhibited; 3) the Wnt/β-catenin pathway is involved in the differentiation of ADSCs into SMCs. After differentiation induction, the synchronized changes in the activation of Wnt/β-catenin signaling and the expression of SMC-specific proteins showed a trend of simultaneous changes, and after the inhibition of the Wnt pathway, the adult muscle differentiation was significantly inhibited.Conclusions: We established a simpler and more efficient method for inducing ADSCs to differentiate into SMCs using TGF-β1 and demonstrated that the Wnt/β-catenin signaling pathway is activated during this process.

Abstract 360: Development of a Human Tissue-Engineered Blood Vessel from Adipose-Derived Stem Cells

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Jaclyn A Brennan ◽  
Julien H Arrizabalaga ◽  
Matthias U Nollert
Keyword(s):  
Stem Cells ◽  
Blood Vessel ◽  
Amniotic Membrane ◽  
Biological Material ◽  
Small Diameter ◽  
Human Amniotic Membrane ◽  
Adipose Derived Stem Cells ◽  
Cell Nuclei ◽  
Static Culture ◽  
Biological Substrate

There exists a clear need for alternative sources of small-diameter vascular grafts for treating the millions of patients who suffer from cardiovascular disease each year. Bypass surgery or replacement of defective vessels is often required to treat coronary heart disease, but there is a limited supply of suitable autologous grafts, and synthetic grafts are ineffective for replacement of small-diameter vessels. Inherent thrombogenicity, compliance mismatch, and limited patency rates are all complications with current options. Tissue engineering has the potential to overcome these limitations by producing a readily-available vascular graft completely from biological material. It is the objective of this study to fabricate such a small-diameter tissue engineered blood vessel (TEBV) by using the human amniotic membrane as a mechanically-sound biological substrate. Our technology begins by differentiating adipose-derived stem cells into smooth muscle cells (SMCs) and seeding them onto a flat sheet of the amniotic membrane. We assessed our hypothesis that several types of SMCs can successfully attach and proliferate on this membrane by fluorescently staining cell nuclei with DAPI and characteristic SMC actin filaments with phallotoxins. After 7 days in static culture, the cell-seeded sheet was wrapped around a 3mm O.D. removable mandrel with 6-7 revolutions to develop a tubular construct with architecture akin to that of a muscular artery’s tunica media layer. After a 2 week static culture period, the TEBV was characterized for its biochemical and mechanical properties. We examined the contraction of the vessel in response to carbachol, a specific agonist for SMCs, and compared our results with the contraction of porcine coronary arteries. Burst pressure and elastic modulus tests were also performed. The mechanical integrity of this construct can be further improved upon its exposure to appropriate physiological conditions in a perfusion bioreactor. We show that adipose-derived endothelial cells (ECs) can be also be seeded into the lumen of this construct to prevent platelet adhesion. In conclusion, we have developed a small-diameter TEBV with off-the-shelf availability using a completely biological material seeded with patient-own stem cells.

scholarly journals Predifferentiated Smooth Muscle-Like Adipose-Derived Stem Cells for Bladder Engineering

2020 ◽  
Vol 26 (17-18) ◽  
pp. 979-992
Author(s):  
Jakub Smolar ◽  
Maya Horst ◽  
Souzan Salemi ◽  
Daniel Eberli
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Adipose Derived Stem Cells

Autophagy is needed during the differentiation of adipose derived stem cells to functional smooth muscle cells for use in bladder engineering

2019 ◽  
Vol 18 (1) ◽  
pp. e1266
Author(s):  
S. Salemi ◽  
D. Haralampieva-Mohr ◽  
B. Kranzbühler ◽  
A. Mortezavi ◽  
T. Sulser ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Adipose Derived Stem Cells
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