scholarly journals The Use of Nanofibrillar Cellulose Hydrogel As a Flexible Three-Dimensional Model to Culture Human Pluripotent Stem Cells

2014 ◽  
Vol 23 (4) ◽  
pp. 380-392 ◽  
Author(s):  
Yan-Ru Lou ◽  
Liisa Kanninen ◽  
Tytti Kuisma ◽  
Johanna Niklander ◽  
Luke A. Noon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Human Pluripotent Stem Cells ◽  
Dimensional Model ◽  
Three Dimensional Model ◽  
Nanofibrillar Cellulose ◽  
Cellulose Hydrogel

scholarly journals A three-dimensional model of human lung development and disease from pluripotent stem cells

2017 ◽  
Vol 19 (5) ◽  
pp. 542-549 ◽  
Author(s):  
Ya-Wen Chen ◽  
Sarah Xuelian Huang ◽  
Ana Luisa Rodrigues Toste de Carvalho ◽  
Siu-Hong Ho ◽  
Mohammad Naimul Islam ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Lung Development ◽  
Human Lung ◽  
Three Dimensional ◽  
Dimensional Model ◽  
Three Dimensional Model

scholarly journals Organoide

2020 ◽  
Keyword(s):  
Stem Cells ◽  
Interdisciplinary Research ◽  
Three Dimensional ◽  
Model Systems ◽  
Gene Technology ◽  
Dimensional Model ◽  
Three Dimensional Model ◽  
Legal Questions ◽  
Wide Debate ◽  
Academy Of Sciences

Organoids are developed from stem cells and serve as three-dimensional model systems for different organs. They have great potential for research and medicine, but also raise philosophical, ethical and legal questions which have rarely been discussed in Germany so far. This thematic study by the interdisciplinary research group (IAG) Gene Technology Report at the Berlin-Brandenburg Academy of Sciences and Humanities offers an overview of current scientific developments, their present and potential application, as well as epistemological, ethical and legal reflections. Hereby, the IAG wants to provide impetus for an interdisciplinary and society-wide debate on this general subject. With contributions by Cantas Alev, Aileen-Diane Bamford, Sina Bartfeld, Andreia S. Batista-Rocha, Ali H. Brivanlou, Thomas Burgold, Cindrilla Chumduri, Stephan Clemens, Emrecan Dilmen, Tobias Erb, Fred Etoc, Melinda B. Fagan, Heiner Fangerau, Boris Fehse, Nina Frey, Tristan Frum, Anne Grapin-Botton, Navin Gupta, Jürgen Hampel, Ferdinand Hucho, Özge Kayisoglu, Rashmiparvathi Keshara, Yung Hae Kim, Bon-Kyoung Koo, Martin Korte, Yaroslav Koshelev, Kai Kretzschmar, Allison Lewis, Lilian Marx-Stölting, Fruzsina Molnár-Gábor, Ryuji Morizane, Stefan Mundlos, Paola Nicolas, Angela Osterheider, In-Hyun Park, Anja Pichl, Sandra Pilat-Carotta, Jens Reich, Marlen Reinschke, Hannah Schickl, Silke Schicktanz, Nicolas Schlegel, Jason R. Spence, Yoshiaki Tanaka, Jochen Taupitz, Isaree Teriyapirom, Margherita Y. Turco, Jörn Walter, Eva Winkler, Martin Zenke.

scholarly journals C-STEM: ENGINEERING NICHE-LIKE MICRO-COMPARTMENTS FOR OPTIMAL AND SCALE-INDEPENDENT EXPANSION OF HUMAN PLURIPOTENT STEM CELLS IN BIOREACTORS

2021 ◽  
Author(s):  
Philippe J.R. Cohen ◽  
Elisa Luquet ◽  
Justine Pletenka ◽  
Andrea Leonard ◽  
Elise Warter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Large Scale ◽  
Scale Up ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Human Pluripotent Stem Cells ◽  
Cell Therapies ◽  
Cellular Source

Human pluripotent stem cells (hPSCs) have emerged as the most promising cellular source for cell therapies. To overcome scale up limitations of classical 2D culture systems, suspension cultures have been developed to meet the need of large-scale culture in regenerative medicine. Despite constant improvements, current protocols relying on the generation of micro-carriers or cell aggregates only achieve moderate amplification performance. Here, guided by reports showing that hPSCs can self-organize in vitro into cysts reminiscent of the epiblast stage in embryo development, we developed a physio-mimetic approach for hPSC culture. We engineered stem cell niche microenvironments inside microfluidics-assisted core-shell microcapsules. We demonstrate that lumenized three-dimensional colonies maximize viability and expansion rates while maintaining pluripotency. By optimizing capsule size and culture conditions, we scale-up this method to industrial scale stirred tank bioreactors and achieve an unprecedented hPSC amplification rate of 282-fold in 6.5 days.

scholarly journals Three-dimensional induction of dorsal, intermediate and ventral spinal cord tissues from human pluripotent stem cells

Development ◽  
2018 ◽  
Vol 145 (16) ◽  
pp. dev162214 ◽  
Author(s):  
Takenori Ogura ◽  
Hideya Sakaguchi ◽  
Susumu Miyamoto ◽  
Jun Takahashi
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Human Pluripotent Stem Cells ◽  
Ventral Spinal Cord

scholarly journals Three-dimensional cardiac microtissues composed of cardiomyocytes and endothelial cells co-differentiated from human pluripotent stem cells

Development ◽  
2017 ◽  
Vol 144 (6) ◽  
pp. 1008-1017 ◽  
Author(s):  
Elisa Giacomelli ◽  
Milena Bellin ◽  
Luca Sala ◽  
Berend J. van Meer ◽  
Leon G. J. Tertoolen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Human Pluripotent Stem Cells
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