Self-Correction of Chromosomal Abnormalities in Human Preimplantation Embryos and Embryonic Stem Cells

2013 ◽  
Vol 22 (17) ◽  
pp. 2449-2456 ◽  
Author(s):  
Masood Bazrgar ◽  
Hamid Gourabi ◽  
Mojtaba Rezazadeh Valojerdi ◽  
Poopak Eftekhari Yazdi ◽  
Hossein Baharvand
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Chromosomal Abnormalities ◽  
Embryonic Stem ◽  
Preimplantation Embryos ◽  
Human Preimplantation Embryos

Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells

2013 ◽  
Vol 20 (9) ◽  
pp. 1131-1139 ◽  
Author(s):  
Liying Yan ◽  
Mingyu Yang ◽  
Hongshan Guo ◽  
Lu Yang ◽  
Jun Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Embryonic Stem ◽  
Preimplantation Embryos ◽  
Rna Seq ◽  
Human Preimplantation Embryos

Recurrent chromosomal abnormalities in human embryonic stem cells

2008 ◽  
Vol 26 (12) ◽  
pp. 1361-1363 ◽  
Author(s):  
Claudia Spits ◽  
Ileana Mateizel ◽  
Mieke Geens ◽  
Afroditi Mertzanidou ◽  
Catherine Staessen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Chromosomal Abnormalities ◽  
Embryonic Stem

scholarly journals Etoposide induces MLL rearrangements and other chromosomal abnormalities in human embryonic stem cells

2009 ◽  
Vol 30 (9) ◽  
pp. 1628-1637 ◽  
Author(s):  
Clara Bueno ◽  
Purificación Catalina ◽  
Gustavo J. Melen ◽  
Rosa Montes ◽  
Laura Sánchez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Chromosomal Abnormalities ◽  
Embryonic Stem

scholarly journals HLA-G Expression in Human Embryonic Stem Cells and Preimplantation Embryos

2011 ◽  
Vol 186 (4) ◽  
pp. 2663-2671 ◽  
Author(s):  
An Verloes ◽  
Hilde Van de Velde ◽  
Joel LeMaoult ◽  
Ileana Mateizel ◽  
Greet Cauffman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Preimplantation Embryos

Derivation characteristics and perspectives for mammalian pluripotential stem cells

2005 ◽  
Vol 17 (2) ◽  
pp. 135 ◽  
Author(s):  
Alan Trounson
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Lines ◽  
Nuclear Transfer ◽  
Cell Mass ◽  
Embryonic Stem ◽  
The Body ◽  
Preimplantation Embryos ◽  
Specific Cell ◽  
Wide Range

Pluripotential stem cells have been derived in mice and primates from preimplantation embryos, postimplantation embryos and bone marrow stroma. Embryonic stem cells established from the inner cell mass of the mouse and human blastocyst can be maintained in an undifferentiated state for a long time by continuous passage on embryonic fibroblasts or in the presence of specific inhibitors of differentiation. Pluripotential stem cells can be induced to differentiate into all the tissues of the body and are able to colonise tissues of interest after transplantation. In mouse models of disease, there are numerous examples of improved tissue function and correction of pathological phenotype. Embryonic stem cells can be derived by nuclear transfer to establish genome-specific cell lines and, in mice, it has been shown that embryonic stem cells are more successfully reprogrammed for development by nuclear transfer than somatic cells. Pluripotential stem cells are a very valuable research resource for the analysis of differentiation pathways, functional genomics, tissue engineering and drug screening. Clinical applications may include both cell therapy and gene therapy for a wide range of tissue injury and degeneration. There is considerable interest in the development of pluripotential stem cell lines in many mammalian species for similar research interests and applications.

scholarly journals Reciprocal Transcriptional Regulation of Pou5f1 and Sox2 via the Oct4/Sox2 Complex in Embryonic Stem Cells

2005 ◽  
Vol 25 (14) ◽  
pp. 6031-6046 ◽  
Author(s):  
Joon-Lin Chew ◽  
Yuin-Han Loh ◽  
Wensheng Zhang ◽  
Xi Chen ◽  
Wai-Leong Tam ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Regulatory Element ◽  
Embryonic Stem ◽  
Preimplantation Embryos ◽  
Distal Enhancer ◽  
Human Escs ◽  
Pluripotent Cells ◽  
Specific Expression ◽  
Endogenous Expression

ABSTRACT Embryonic stem cells (ESCs) are pluripotent cells that can either self-renew or differentiate into many cell types. Oct4 and Sox2 are transcription factors essential to the pluripotent and self-renewing phenotypes of ESCs. Both factors are upstream in the hierarchy of the transcription regulatory network and are partners in regulating several ESC-specific genes. In ESCs, Sox2 is transcriptionally regulated by an enhancer containing a composite sox-oct element that Oct4 and Sox2 bind in a combinatorial interaction. It has previously been shown that Pou5f1, the Oct4 gene, contains a distal enhancer imparting specific expression in both ESCs and preimplantation embryos. Here, we identify a composite sox-oct element within this enhancer and show that it is involved in Pou5f1 transcriptional activity in ESCs. In vitro experiments with ESC nuclear extracts demonstrate that Oct4 and Sox2 interact specifically with this regulatory element. More importantly, by chromatin immunoprecipitation assay, we establish that both Oct4 and Sox2 bind directly to the composite sox-oct elements in both Pou5f1 and Sox2 in living mouse and human ESCs. Specific knockdown of either Oct4 or Sox2 by RNA interference leads to the reduction of both genes' enhancer activities and endogenous expression levels in addition to ESC differentiation. Our data uncover a positive and potentially self-reinforcing regulatory loop that maintains Pou5f1 and Sox2 expression via the Oct4/Sox2 complex in pluripotent cells.

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