Expression of mRNAs for DNA methyltransferases and methyl-CpG-binding proteins in the human female germ line, preimplantation embryos, and embryonic stem cells

2004 ◽  
Vol 67 (3) ◽  
pp. 323-336 ◽  
Author(s):  
J. Huntriss ◽  
M. Hinkins ◽  
B. Oliver ◽  
S.E. Harris ◽  
J.C. Beazley ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Binding Proteins ◽  
Germ Line ◽  
Embryonic Stem ◽  
Dna Methyltransferases ◽  
Preimplantation Embryos ◽  
Human Female ◽  
Female Germ Line

Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells

1990 ◽  
Vol 10 (12) ◽  
pp. 6755-6758
Author(s):  
B R Stanton ◽  
S W Reid ◽  
L F Parada
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Homologous Recombination ◽  
Embryonic Development ◽  
Cell Lines ◽  
Germ Line ◽  
Es Cells ◽  
Embryonic Stem ◽  
Es Cell ◽  
Germ Line Transmission

We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.

scholarly journals Developmental remodelling of non-CG methylation at satellite DNA repeats

2020 ◽  
Vol 48 (22) ◽  
pp. 12675-12688 ◽  
Author(s):  
Samuel E Ross ◽  
Allegra Angeloni ◽  
Fan-Suo Geng ◽  
Alex de Mendoza ◽  
Ozren Bogdanovic
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transcriptional Repression ◽  
Dna Methyltransferase ◽  
Embryonic Stem ◽  
Dna Methyltransferases ◽  
Dna Repeats ◽  
Satellite Repeats ◽  
Cg Dinucleotides ◽  
The Brain

Abstract In vertebrates, DNA methylation predominantly occurs at CG dinucleotides however, widespread non-CG methylation (mCH) has been reported in mammalian embryonic stem cells and in the brain. In mammals, mCH is found at CAC trinucleotides in the nervous system, where it is associated with transcriptional repression, and at CAG trinucleotides in embryonic stem cells, where it positively correlates with transcription. Moreover, CAC methylation appears to be a conserved feature of adult vertebrate brains. Unlike any of those methylation signatures, here we describe a novel form of mCH that occurs in the TGCT context within zebrafish mosaic satellite repeats. TGCT methylation is inherited from both male and female gametes, remodelled during mid-blastula transition, and re-established during gastrulation in all embryonic layers. Moreover, we identify DNA methyltransferase 3ba (Dnmt3ba) as the primary enzyme responsible for the deposition of this mCH mark. Finally, we observe that TGCT-methylated repeats are specifically associated with H3K9me3-marked heterochromatin suggestive of a functional interplay between these two gene-regulatory marks. Altogether, this work provides insight into a novel form of vertebrate mCH and highlights the substrate diversity of vertebrate DNA methyltransferases.

scholarly journals Effective Production of Microinjectable Blastocysts for Germ-Line Transmission of Embryonic Stem Cells

2004 ◽  
Vol 53 (5) ◽  
pp. 475-477
Author(s):  
Sun-Uk KIM ◽  
Ying-Hao HAN ◽  
Tae-Hoon LEE ◽  
Byung-Hwa HYUN ◽  
Sang-Ho LEE ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Line ◽  
Embryonic Stem ◽  
Germ Line Transmission

Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells

Cell ◽  
1989 ◽  
Vol 56 (2) ◽  
pp. 313-321 ◽  
Author(s):  
Simon Thompson ◽  
Alan R. Clarke ◽  
Angela M. Pow ◽  
Martin L. Hooper ◽  
David W. Melton
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Germ Line ◽  
Embryonic Stem ◽  
Hprt Gene ◽  
Germ Line Transmission

scholarly journals Molecular control of pluripotency and germ line competency in chicken embryonic stem cells

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S106-S106 ◽  
Author(s):  
F Lavial ◽  
H Acloque ◽  
E Bachelard ◽  
G Montillet ◽  
A Nieto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Line ◽  
Embryonic Stem ◽  
Molecular Control ◽  
Chicken Embryonic Stem Cells

scholarly journals Germ-Line Contribution of Embryonic Stem Cells in Chimeric Mice: Influence of Karyotype and In Vitro Differentiation Ability.

1997 ◽  
Vol 46 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Hiroshi SUZUKI ◽  
Nobuo KAMADA ◽  
Otoya UEDA ◽  
Kouichi JISHAGE ◽  
Yukiko KURIHARA ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Line ◽  
Embryonic Stem ◽  
Chimeric Mice ◽  
In Vitro Differentiation
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