scholarly journals Long Noncoding RNAs: New Players in the Molecular Mechanism for Maintenance and Differentiation of Pluripotent Stem Cells

2013 ◽  
Vol 22 (16) ◽  
pp. 2240-2253 ◽  
Author(s):  
Suman Ghosal ◽  
Shaoli Das ◽  
Jayprokas Chakrabarti
Keyword(s):  
Stem Cells ◽  
Molecular Mechanism ◽  
Pluripotent Stem Cells ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas

scholarly journals When Long Noncoding RNAs Meet Genome Editing in Pluripotent Stem Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-13
Author(s):  
Fuquan Chen ◽  
Jiaojiao Ji ◽  
Jian Shen ◽  
Xinyi Lu
Keyword(s):  
Stem Cells ◽  
Transcriptional Regulation ◽  
Genome Editing ◽  
Pluripotent Stem Cells ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Biological Processes ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
The Past

Most of the human genome can be transcribed into RNAs, but only a minority of these regions produce protein-coding mRNAs whereas the remaining regions are transcribed into noncoding RNAs. Long noncoding RNAs (lncRNAs) were known for their influential regulatory roles in multiple biological processes such as imprinting, dosage compensation, transcriptional regulation, and splicing. The physiological functions of protein-coding genes have been extensively characterized through genome editing in pluripotent stem cells (PSCs) in the past 30 years; however, the study of lncRNAs with genome editing technologies only came into attentions in recent years. Here, we summarize recent advancements in dissecting the roles of lncRNAs with genome editing technologies in PSCs and highlight potential genome editing tools useful for examining the functions of lncRNAs in PSCs.

scholarly journals Long noncoding RNAs sustain high expression of exogenous Oct4 by sponging miRNA during reprogramming

2019 ◽  
Author(s):  
Qingran Kong ◽  
Xiaolei Zhang ◽  
Jiaming Zhang ◽  
Kailun Zheng ◽  
Heng Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Circular Rnas ◽  
Low Grade ◽  
Oct4 Expression ◽  
Competing Endogenous Rnas ◽  
Cellular Context ◽  
Induced Pluripotent

AbstractLong noncoding RNAs (lncRNAs) modulate gene expression as competing endogenous RNAs (ceRNAs) via sponging microRNAs (miRNAs). However, the extent and functional consequences of ceRNAs in diverse cellular context still need to be proven. Using a doxycycline inducible expression of Yamanaka four factors to generate induced pluripotent stem cells (iPSCs) from mouse embryonic fibroblasts (MEFs), we found the miRNAs from MEFs remained highly expressed from day 0 to day 6 after doxycycline induction; unexpectedly, many genes targeted by these miRNAs were actually up-regulated; meanwhile, long intergenic noncoding RNAs (lincRNAs) and circular RNAs (circRNAs) which have complementary binding sites with the miRNAs were highly expressed, indicating lincRNAs and circRNAs (linc/circRNAs) may serve as sponges for miRNAs to block their activities during reprogramming. Intriguingly, the knockdown of the linc/circRNAs sponging the miRNAs targeting Oct4 mRNA resulted in down-regulation of exogenous Oct4 expression, decrease of reprogramming efficiency, and low-grade chimera forming iPSCs. Our results suggest that the ceRNA network plays an important role in reprogramming somatic cells to pluripotent stem cells.

Differential expression of long noncoding RNAs in SDF-1α-induced dental pulp stem cells

2021 ◽  
Author(s):  
min xiao ◽  
Bo Yao ◽  
Xiaohan Mei ◽  
yu bai ◽  
Jueyu Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Rho Gtpase ◽  
Noncoding Rnas ◽  
Dental Pulp Stem Cells ◽  
Long Noncoding Rnas ◽  
Nodule Formation ◽  
Related Factor ◽  
Altered Expression ◽  
Odontogenic Differentiation

Abstract Background SDF-1α cotreatment was shown to have synergistic effects on BMP-2-induced odontogenic differentiation of human apical dental papillary stem cells (SCAP) both in vitro and in vivo. Long noncoding RNAs (lncRNAs) have an important role in the odontogenic differentiation of dental pulp stem cells (DPSCs). Methods We examined the altered expression of lncRNAs in SDF-1α-induced odontogenic differentiation of DPSCs by lncRNA microarray and quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses. Alterations in lncRNA expression during odontogenic differentiation of DPSCs were identified. Moreover, bioinformatic analysis [Gene Ontology (GO) analysis and coding-noncoding gene coexpression (CNC) analysis] was conducted to predict the interactions of lncRNAs and identify core regulatory factors in SDF-1α-induced odontogenic differentiation of DPSCs. Results The microarray analysis identified 206 differentially expressed lncRNAs (134 lncRNAs with upregulated expression and 72 with downregulated expression) at 7 days post‑treatment. The data demonstrated that one lncRNA, AC080037.1, regulates SDF-1α-induced odontogenic differentiation of DPSCs. Our data showed that lncRNA AC080037.1 siRNA suppresses DPSCs migration and the expression of Rho GTPase induced by SDF-1α. Moreover, AC080037.1 knockdown significantly affected SDF-1α- and BMP-2-induced mineralized nodule formation and strongly suppressed Runt-related factor-2 (RUNX-2), DMP-1 and DSPP expression in DPSCs. Conclusions Our

Long noncoding RNAs in gut stem cells

2018 ◽  
Vol 20 (10) ◽  
pp. 1106-1107 ◽  
Author(s):  
Pantelis Hatzis ◽  
Hugo J. G. Snippert
Keyword(s):  
Stem Cells ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas

scholarly journals Noncoding RNAs: new insights into the odontogenic differentiation of dental tissue-derived mesenchymal stem cells

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Fuchun Fang ◽  
Kaiying Zhang ◽  
Zhao Chen ◽  
Buling Wu
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Signaling Pathways ◽  
Dental Pulp ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Substantial Part ◽  
Dental Tissue ◽  
Odontogenic Differentiation ◽  
Dentin Regeneration

Abstract Odontoblasts are cells that contribute to the formation of the dental pulp complex. The differentiation of dental tissue-derived mesenchymal stem cells into odontoblasts comprises many factors and signaling pathways. Noncoding RNAs (ncRNAs), comprising a substantial part of poly-A tail mature RNAs, are considered “transcriptional noise.” Emerging evidence has shown that ncRNAs have key functions in the differentiation of mesenchymal stem cells. In this review, we discussed two major types of ncRNAs, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), in terms of their role in the odontogenic differentiation of dental tissue-derived stem cells. Recent findings have demonstrated important functions for miRNAs and lncRNAs in odontogenic differentiation. It is expected that ncRNAs will become promising therapeutic targets for dentin regeneration based on stem cells.

Long Noncoding RNAs: New Players in the Osteogenic Differentiation of Bone Marrow- and Adipose-Derived Mesenchymal Stem Cells

2018 ◽  
Vol 14 (3) ◽  
pp. 297-308 ◽  
Author(s):  
Qiaolin Yang ◽  
Lingfei Jia ◽  
Xiaobei Li ◽  
Runzhi Guo ◽  
Yiping Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas
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