scholarly journals Umbilical Cord Blood-Derived Mesenchymal Stem Cells Inhibit, But Adipose Tissue-Derived Mesenchymal Stem Cells Promote, Glioblastoma Multiforme Proliferation

2013 ◽  
Vol 22 (9) ◽  
pp. 1370-1386 ◽  
Author(s):  
Keiko Akimoto ◽  
Kenichi Kimura ◽  
Masumi Nagano ◽  
Shingo Takano ◽  
Georgina To'a Salazar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Glioblastoma Multiforme ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood

scholarly journals Current Status of Canine Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Veterinary Medicine

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Tania Sultana ◽  
Soojung Lee ◽  
Hun-Young Yoon ◽  
Jeong Ik Lee
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Veterinary Medicine ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Ethical Issues ◽  
Treatment Strategies ◽  
Current Status

Stem cell therapy has prompted the expansion of veterinary medicine both experimentally and clinically, with the potential to contribute to contemporary treatment strategies for various diseases and conditions for which limited or no therapeutic options are presently available. Although the application of various types of stem cells, such as bone marrow-derived mesenchymal stem cells (BM-MSCs), adipose tissue-derived mesenchymal stem cells (AT-MSCs), and umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs), has promising potential to improve the health of different species, it is crucial that the benefits and drawbacks are completely evaluated before use. Umbilical cord blood (UCB) is a rich source of stem cells; nonetheless, isolation of mesenchymal stem cells (MSCs) from UCB presents technical challenges. Although MSCs have been isolated from UCB of diverse species such as human, equine, sheep, goat, and canine, there are inherent limitations of using UCB from these species for the expansion of MSCs. In this review, we investigated canine UCB (cUCB) and compared it with UCB from other species by reviewing recent articles published from February 2003 to June 2017 to gain an understanding of the limitations of cUCB in the acquisition of MSCs and to determine other suitable sources for the isolation of MSCs from canine. Our review indicates that cUCB is not an ideal source of MSCs because of insufficient volume and ethical issues. However, canine reproductive organs discarded during neutering may help broaden our understanding of effective isolation of MSCs. We recommend exploring canine reproductive and adipose tissue rather than UCB to fulfill the current need in veterinary medicine for the well-designed and ethically approved source of MSCs.

Dissimilar Differentiation of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, and Adipose Tissue

2008 ◽  
Vol 233 (7) ◽  
pp. 901-913 ◽  
Author(s):  
C. K. Rebelatto ◽  
A. M. Aguiar ◽  
M. P. Moretão ◽  
A. C. Senegaglia ◽  
P. Hansen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood

scholarly journals Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep

2011 ◽  
Vol 26 (4) ◽  
pp. 267-273 ◽  
Author(s):  
Leandro Fadel ◽  
Brunno Rosa Viana ◽  
Matheus Levi Tajra Feitosa ◽  
Anna Caroline Mazeto Ercolin ◽  
Kelly Cristine Santos Roballo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Growth Curve ◽  
Umbilical Cord Blood ◽  
Umbilical Blood ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

PURPOSE: To evaluate different protocols to isolate stem cells from ovine umbilical cord blood and adipose tissue. METHODS: There were used 5 samples of umbilical blood and 5 samples of perirenal adipose tissue from 10 female sheep. All the samples were obtained through surgery, to harvest aseptic samples. There were used 3 protocols for obtainment and culture of umbilical cord blood stem cells and 4 protocols for ovine adipose tissue stem cells. RESULTS: It was possible to observe only one successful protocol for the obtainment of umbilical cord blood stem cells. When analyzing the techniques used to obtain adipose tissue stem cells, only one of the methods was effective as well. Through colony forming unit assay, there were obtained 58 colonies of cells after seven days in culture. Flow citometry tests revealed the cells were positive to CD44 and exhibited negative reaction to CD38, CD45, CD41/61. These cells showed a growth curve with very well defined phases LOG, LAG and PLATEAU. This phases are typically seem in mesenchymal stem cells growth curves. CONCLUSIONS: The isolation and culture of mesenchymal stem cells from ovine umbilical cord blood are complex and request more detailed assays. Stem cells from fat tissue sheep showed mesenchymal characteristics, according to their cell growth curve, ability to origin colonies of fibroblastoid cells and positive reactivity with the antibody CD44 by flow citometry.

scholarly journals Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose Tissue

Stem Cells ◽  
2006 ◽  
Vol 24 (5) ◽  
pp. 1294-1301 ◽  
Author(s):  
Susanne Kern ◽  
Hermann Eichler ◽  
Johannes Stoeve ◽  
Harald Klüter ◽  
Karen Bieback
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Comparative Analysis ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood

Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood

2005 ◽  
Vol 33 (11) ◽  
pp. 1402-1416 ◽  
Author(s):  
Wolfgang Wagner ◽  
Frederik Wein ◽  
Anja Seckinger ◽  
Maria Frankhauser ◽  
Ute Wirkner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Adipose Tissue
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