scholarly journals A Chimeric Vitronectin: IGF-I Protein Supports Feeder-Cell-Free and Serum-Free Culture of Human Embryonic Stem Cells

2010 ◽  
Vol 19 (9) ◽  
pp. 1297-1305 ◽  
Author(s):  
Kerry J. Manton ◽  
Sean Richards ◽  
Derek Van Lonkhuyzen ◽  
Luke Cormack ◽  
David Leavesley ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Feeder Cell ◽  
Serum Free ◽  
Igf I

scholarly journals Differentiation of Human Embryonic Stem Cells into Engrafting Myogenic Precursor Cells

2019 ◽  
Vol 1 (1) ◽  
pp. 01-05
Author(s):  
Stalin Reddy Challa ◽  
Swathi Goli
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Precursor Cells ◽  
Functional Improvement ◽  
Serum Free ◽  
Myogenic Stem Cells ◽  
Serum Free Conditions ◽  
Myogenic Precursor

Degenerative muscle diseases affect muscle tissue integrity and function. Human embryonic stem cells (hESC) are an attractive source of cells to use in regenerative therapies due to their unlimited capacity to divide and ability to specialize into a wide variety of cell types. A practical way to derive therapeutic myogenic stem cells from hESC is lacking. In this study, we demonstrate the development of two serum-free conditions to direct the differentiation of hESC towards a myogenic precursor state. Using TGFß and PI3Kinase inhibitors in combination with bFGF we showed that one week of differentiation is sufficient for hESC to specialize into PAX3+/PAX7+ myogenic precursor cells. These cells also possess the capacity to further differentiate in vitro into more specialized myogenic cells that express MYOD, Myogenin, Desmin and MYHC, and showed engraftment in vivo upon transplantation in immunodeficient mice. Ex vivo myomechanical studies of dystrophic mouse hindlimb muscle showed functional improvement one month post-transplantation. In summary, this study describes a promising system to derive engrafting muscle precursor cells solely using chemical substances in serum-free conditions and without genetic manipulation.

Expansion of human embryonic stem cells in defined serum‐free medium devoid of animal‐derived products

2005 ◽  
Vol 91 (6) ◽  
pp. 688-698 ◽  
Author(s):  
Yan Li ◽  
Sandra Powell ◽  
Elisa Brunette ◽  
Jane Lebkowski ◽  
Ramkumar Mandalam
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Chemically defined serum-free conditions for cartilage regeneration from human embryonic stem cells

Life Sciences ◽  
2016 ◽  
Vol 164 ◽  
pp. 9-14 ◽  
Author(s):  
Dandan Yang ◽  
Shubin Chen ◽  
Changzhao Gao ◽  
Xiaobo Liu ◽  
Yulai Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cartilage Regeneration ◽  
Serum Free ◽  
Serum Free Conditions
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