scholarly journals Unique Gene Expression Signature by Human Embryonic Stem Cells Cultured Under Serum-Free Conditions Correlates with Their Enhanced and Prolonged Growth in an Undifferentiated Stage

Stem Cells ◽  
2006 ◽  
Vol 24 (1) ◽  
pp. 151-167 ◽  
Author(s):  
Heli Skottman ◽  
Anne-Marie Strömberg ◽  
Eija Matilainen ◽  
Jose Inzunza ◽  
Outi Hovatta ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Gene Expression Signature ◽  
Serum Free ◽  
Unique Gene ◽  
Serum Free Conditions ◽  
Cells Cultured

scholarly journals Differentiation of Human Embryonic Stem Cells into Engrafting Myogenic Precursor Cells

2019 ◽  
Vol 1 (1) ◽  
pp. 01-05
Author(s):  
Stalin Reddy Challa ◽  
Swathi Goli
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Precursor Cells ◽  
Functional Improvement ◽  
Serum Free ◽  
Myogenic Stem Cells ◽  
Serum Free Conditions ◽  
Myogenic Precursor

Degenerative muscle diseases affect muscle tissue integrity and function. Human embryonic stem cells (hESC) are an attractive source of cells to use in regenerative therapies due to their unlimited capacity to divide and ability to specialize into a wide variety of cell types. A practical way to derive therapeutic myogenic stem cells from hESC is lacking. In this study, we demonstrate the development of two serum-free conditions to direct the differentiation of hESC towards a myogenic precursor state. Using TGFß and PI3Kinase inhibitors in combination with bFGF we showed that one week of differentiation is sufficient for hESC to specialize into PAX3+/PAX7+ myogenic precursor cells. These cells also possess the capacity to further differentiate in vitro into more specialized myogenic cells that express MYOD, Myogenin, Desmin and MYHC, and showed engraftment in vivo upon transplantation in immunodeficient mice. Ex vivo myomechanical studies of dystrophic mouse hindlimb muscle showed functional improvement one month post-transplantation. In summary, this study describes a promising system to derive engrafting muscle precursor cells solely using chemical substances in serum-free conditions and without genetic manipulation.

Chemically defined serum-free conditions for cartilage regeneration from human embryonic stem cells

Life Sciences ◽  
2016 ◽  
Vol 164 ◽  
pp. 9-14 ◽  
Author(s):  
Dandan Yang ◽  
Shubin Chen ◽  
Changzhao Gao ◽  
Xiaobo Liu ◽  
Yulai Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cartilage Regeneration ◽  
Serum Free ◽  
Serum Free Conditions

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression

scholarly journals Conditional Gene Expression in Human Embryonic Stem Cells

Stem Cells ◽  
2007 ◽  
Vol 25 (6) ◽  
pp. 1490-1497 ◽  
Author(s):  
Ludovic Vallier ◽  
Morgan Alexander ◽  
Roger Pedersen
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Conditional Gene Expression

Expansion of human embryonic stem cells in defined serum‐free medium devoid of animal‐derived products

2005 ◽  
Vol 91 (6) ◽  
pp. 688-698 ◽  
Author(s):  
Yan Li ◽  
Sandra Powell ◽  
Elisa Brunette ◽  
Jane Lebkowski ◽  
Ramkumar Mandalam
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free
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