Functional Module Analysis Reveals Differential Osteogenic and Stemness Potentials in Human Mesenchymal Stem Cells from Bone Marrow and Wharton's Jelly of Umbilical Cord

2010 ◽  
Vol 19 (12) ◽  
pp. 1895-1910 ◽  
Author(s):  
Jui-Yu Hsieh ◽  
Yu-Show Fu ◽  
Shing-Jyh Chang ◽  
Yang-Hwei Tsuang ◽  
Hsei-Wei Wang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Functional Module ◽  
Human Mesenchymal Stem Cells ◽  
Wharton’S Jelly ◽  
Module Analysis ◽  
Wharton's Jelly

Lectin Profile Variation in Mesenchymal Stem Cells Derived from Different Sources

2019 ◽  
Vol 208 (3-4) ◽  
pp. 101-112
Author(s):  
Tahereh Talaei-Khozani ◽  
Fatemeh Aleahmad ◽  
Ameneh Bazrafshan ◽  
Elham Aliabadi ◽  
Zahra Vojdani
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Human Mesenchymal Stem Cells ◽  
Cell Types ◽  
Staining Intensity ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Promising Source

Human mesenchymal stem cells (MSCs), a promising source of stem cells for regenerative medicine, have different morphological and functional characteristics. Carbohydrate moieties on the cell surface play an important role, including cell-cell interaction and cell recognition. The objective of this study was to determine possible differences in glycoconjugate distribution patterns of MSCs derived from various sources. MSCs were isolated from adipose tissue, bone marrow, Wharton’s jelly, and cord blood. Then, they were stained with FITC-conjugated wheat germ agglutinin (WGA), peanut agglutinin (PNA), concanavalin A (ConA), Ulex europaeus (UEA), Dolichos biflorus (DBA), and Atto-488 conjugated Phytolacca americana (PWM) lectins. The intensity of the reactions was scored using ImageJ software. Flow cytometry was performed to detect the expression of the endothelial marker CD144. The obtained data were analyzed by ANOVA and LSD. Cord blood-derived MSCs showed the most significant staining intensities with all lectins. All MSCs were also moderately stained with PNA. Bone marrow-derived MSCs failed to react with UEA, DBA, and ConA. Wharton’s jelly-derived MSCs could also not be stained with ConA. Cord blood-derived MSCs contained 2 subpopulations: osteoclast- and fibroblast-like cells. Both lectin staining intensity and distribution pattern were different in these 2 cell types; therefore, the central part of osteoclast-like cells stained more intensive with PNA and PWM, while that part in fibroblast-like cells stained more intensive with ConA. None of them expressed CD144. The glycoconjugate content of MSCs derived from various sources is different.

scholarly journals Exosomes derived from human umbilical cord Wharton's jelly mesenchymal stem cells ameliorate experimental lymphedema

2021 ◽  
Vol 11 (7) ◽  
Author(s):  
Zhao Ting ◽  
Yan Zhi‐xin ◽  
Tan You‐wen ◽  
Yang Fu‐ji ◽  
Sun Hui ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Wharton's Jelly

scholarly journals Enhanced Chondrogenic Differentiation of Human Umbilical Cord Wharton's Jelly Derived Mesenchymal Stem Cells by GSK-3 Inhibitors

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0168059 ◽  
Author(s):  
Prapot Tanthaisong ◽  
Sumeth Imsoonthornruksa ◽  
Apichart Ngernsoungnern ◽  
Piyada Ngernsoungnern ◽  
Mariena Ketudat-Cairns ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Chondrogenic Differentiation ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Wharton's Jelly

382 ISOLATION AND CHARACTERIZATION OF SIZE-SIEVED MESENCHYMAL STEM CELLS FROM PERIVASCULAR AND INTERVASCULAR WHARTON'S JELLY OF HORSE UMBILICAL CORD

2010 ◽  
Vol 22 (1) ◽  
pp. 347 ◽  
Author(s):  
A. Corradetti ◽  
A. Lange Consiglio ◽  
M. Barucca ◽  
F. Cremonesi ◽  
D. Bizzaro
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Clinical Importance ◽  
Wharton’S Jelly ◽  
Small Cells ◽  
Time Range ◽  
Homogeneous Population ◽  
Wharton's Jelly ◽  
Isolation And Characterization

Horse umbilical cord has recently been suggested as a potential source for mesenchymal stem cells (MSC). Despite their clinical importance for treating injuries to musculoskeletal tissues, there is still not a well-defined protocol for the isolation and expansion of MSC in culture. Literature shows few experiments conducted on equine MSC; in these experiments cells are isolated primarily by their tight adherence to culture plastic dishes. These cells are initially heterogeneous so we aimed to separate homogeneous subpopulations of MSC using multi-dishes with transwell inserts of 8 μm pores. After digestion of perivascular and intervascular Wharton’s jelly with collagenase (0.75 mg mL-1) for 16 h at 37°C, 12 primary cell cultures from 3 animals were obtained. Cells were plated at a density of 106 cells/cm2 on these culture dishes. The lower (8μm) and upper populations of perivascular and intervascular cells, cultured in fetal bovine serum-supplemented DMEM-HG, with EGF, were studied for their morphology, renewal capacity, mesenchymal markers expression, and differentiating potential. Cells with less than an 8 μm diameter that adhered to the lower plate surface were a morphological homogeneous population if compared with the upper larger sized population of either perivascular or intervascular jelly. Every subpopulation steadily proliferated over the passages studied, without spontaneous differentiation, reaching confluence even after 10 passages. The large cells of the perivascular portion propagated slowly and passed 16.58 cell population doublings (PD) after 31 days, whereas in the same time range, the small cells reached 19.49 cell PD. After the seventh passage, the proliferating traits of the 2 cell populations became similar. As a control, the unsieved perivascular portion passed 8.54 cell PD. On the contrary, in the intervascular portion, the large cells propagated more rapidly with respect to the small ones (20.53 v. 13.66 cell PD) and the unsieved control (9.42 cell PD). The fibroblast colony forming unit (CFU-F) assay supported these differences with greater CFU-F for the small perivascular cells and the large intervascular cells (the rates were, respectively, 1:133 and 1:106). As shown by RT-PCR, every subpopulation was positive for MSC markers (CD105, CD 44, CD 29) and CD34 negative. Osteogenic differentiation of each subpopulation was confirmed by von Kossa stain and osteocalcin mRNA expression after 10 days of induction. At this time point, only intervascular cells expressed osteopontin, suggesting an earlier expression of this marker in these cells. We suggest that in the perivascular portion, the large cells are mature MSC and the smaller ones are recycling stem cells and that in the intervascular fraction, MSC do not divide rapidly until after they are separated from non-MSC of whole primary culture. The size-sieving procedure is a simple and effective method to isolate more proliferative MSC.

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