Differentiating Normal and Basal Cell Carcinoma Human Skin Tissues In Vitro Using Dispersive Raman Spectroscopy: A Comparison Between Principal Components Analysis and Simplified Biochemical Models

2010 ◽  
Vol 28 (S1) ◽  
pp. S-119-S-127 ◽  
Author(s):  
Benito Bodanese ◽  
Landulfo Silveira ◽  
Regiane Albertini ◽  
Renato Amaro Zângaro ◽  
Marcos Tadeu Tavares Pacheco
Keyword(s):  
Raman Spectroscopy ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Principal Components Analysis ◽  
Human Skin ◽  
Basal Cell ◽  
Principal Components ◽  
Components Analysis

Principal components analysis of FT-Raman spectra of ex vivo basal cell carcinoma

2004 ◽  
Author(s):  
Airton A. Martin ◽  
Renata A. Bitar Carter ◽  
Lilian de Oliveira Nunes ◽  
Emilia A. Loschiavo Arisawa ◽  
Landulfo Silveira, Jr.
Keyword(s):  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Principal Components Analysis ◽  
Raman Spectra ◽  
Basal Cell ◽  
Principal Components ◽  
Ex Vivo ◽  
Components Analysis ◽  
Ft Raman

Discriminating Neoplastic and Normal Brain Tissues in Vitro Through Raman Spectroscopy: A Principal Components Analysis Classification Model

2013 ◽  
Vol 31 (12) ◽  
pp. 595-604 ◽  
Author(s):  
Ricardo Pinto Aguiar ◽  
Landulfo Silveira ◽  
Edgar Teixeira Falcão ◽  
Marcos Tadeu Tavares Pacheco ◽  
Renato Amaro Zângaro ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Principal Components Analysis ◽  
Principal Components ◽  
Classification Model ◽  
Normal Brain ◽  
Components Analysis ◽  
Brain Tissues

scholarly journals Discrimination of Basal Cell Carcinoma and Melanoma from Normal Skin Biopsies in Vitro Through Raman Spectroscopy and Principal Component Analysis

2012 ◽  
Vol 30 (7) ◽  
pp. 381-387 ◽  
Author(s):  
Benito Bodanese ◽  
Fabrício Luiz Silveira ◽  
Renato Amaro Zângaro ◽  
Marcos Tadeu T. Pacheco ◽  
Carlos Augusto Pasqualucci ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Raman Spectroscopy ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Normal Skin ◽  
Principal Component ◽  
Component Analysis ◽  
Skin Biopsies

Formulation, Characterization and In vitro Cytotoxicity of 5-Fluorouracil Loaded Polymeric Electrospun Nanofibers for the Treatment of Skin Cancer

2019 ◽  
Vol 13 (2) ◽  
pp. 114-128 ◽  
Author(s):  
Gayatri Patel ◽  
Bindu K.N. Yadav
Keyword(s):  
Skin Cancer ◽  
Basal Cell Carcinoma ◽  
Cell Viability ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Fiber Diameter ◽  
Electrospun Nanofibers ◽  
In Vitro Cytotoxicity ◽  
Fractional Factorial

Background: The purpose of this study was to formulate, characterize and conduct in vitro cytotoxicity of 5-fluorouracil loaded polymeric electrospun nanofibers for the treatment of skin cancer. The patents on electrospun nanofibers (US9393216B2), (US14146252), (WO2015003155A1) etc. helped in the selection of polymers and method for the preparation of nanofibers. Methods: In the present study, the fabrication of nanofibers was done using a blend of chitosan with polyvinyl alcohol and processed using the electrospinning technique. 5-fluorouracil with known chemotherapeutic potential in the treatment of skin cancer was used as a drug carrier. 24-1 fractional factorial screening design was employed to study the effect of independent variables like the concentration of the polymeric solution, applied voltage (kV), distance (cm), flow rate (ml / hr) on dependent variables like % entrapment efficiency and fiber diameter. Results: Scanning electron microscopy was used to characterize fiber diameter and morphology. Results showed that the fiber diameter of all batches was found in the range of 100-200 nm. The optimized batch results showed the fiber diameter of 162.7 nm with uniform fibers. The tensile strength obtained was 190±37 Mpa. Further in vitro and ex vivo drug release profile suggested a controlled release mechanism for an extended period of 24 hr. The 5-fluorouracil loaded electrospun nanofibers were found to decrease cell viability up to ≥50% over 24 hr, with the number of cells dropping by ~ 10% over 48 hr. As the cell viability was affected by the release of 5-fluorouracil, we believe that electrospun nanofibers are a promising drug delivery system for the treatment of Basal Cell Carcinoma (BCC) skin cancer. Conclusion: These results demonstrate the possibility of delivering 5-Fluorouracil loaded electrospun nanofiber to skin with enhanced encapsulation efficiency indicating the effectiveness of the formulation for the treatment of basal cell carcinoma type of skin cancer.

scholarly journals Formulations Based on Drug Loaded Aptamer-Conjugated Liposomes as a Viable Strategy for the Topical Treatment of Basal Cell Carcinoma—In Vitro Tests

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 866
Author(s):  
Anca N. Cadinoiu ◽  
Delia M. Rata ◽  
Leonard I. Atanase ◽  
Cosmin T. Mihai ◽  
Simona E. Bacaita ◽  
...  
Keyword(s):  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Topical Treatment ◽  
Skin Irritation ◽  
In Vitro Tests ◽  
Liposomal Drug ◽  
Liposomal Formulations ◽  
As1411 Aptamer

Topical liposomal drug formulations containing AS1411-aptamer conjugated liposomes were designed to deliver in a sustained way the 5-fluorouracil to the tumor site but also to increase the compliance of patients with basal cell carcinoma. The 5-fluorouracil penetrability efficiency through the Strat-M membrane and the skin irritation potential of the obtained topical liposomal formulations were evaluated in vitro and the Korsmeyer Peppas equation was considered as the most appropriate to model the drug release. Additionally, the efficiency of cytostatic activity for targeted antitumor therapy and the hemolytic capacity were performed in vitro. The obtained results showed that the optimal liposomal formulation is a crosslinked gel based on sodium alginate and hyaluronic acid containing AS1411-aptamer conjugated liposomes loaded with 5-fluorouracil, which appeared to have favorable biosafety effects and may be used as a new therapeutic approach for the topical treatment of basal cell carcinoma.

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