Exposure to Sublethal Ciprofloxacin Induces Resistance to Ciprofloxacin and Cross-Antibiotics, and Reduction of Fitness, Biofilm Formation, and Apx Toxin Secretion in Actinobacillus pleuropneumoniae

2021 ◽  
Author(s):  
Fangfang Guo ◽  
Jie Guo ◽  
Yifang Cui ◽  
Xiaoya Cao ◽  
Hongzhuan Zhou ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Actinobacillus Pleuropneumoniae ◽  
Toxin Secretion

scholarly journals The ClpP Protease Is Required for the Stress Tolerance and Biofilm Formation in Actinobacillus pleuropneumoniae

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53600 ◽  
Author(s):  
Fang Xie ◽  
Yanhe Zhang ◽  
Gang Li ◽  
Long Zhou ◽  
Siguo Liu ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Biofilm Formation ◽  
Actinobacillus Pleuropneumoniae

scholarly journals Absence of TolC Impairs Biofilm Formation in Actinobacillus pleuropneumoniae by Reducing Initial Attachment

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0163364 ◽  
Author(s):  
Ying Li ◽  
Sanjie Cao ◽  
Luhua Zhang ◽  
Jianlin Yuan ◽  
Gee W. Lau ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Actinobacillus Pleuropneumoniae ◽  
Initial Attachment

scholarly journals Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σE and H-NS

2010 ◽  
Vol 192 (9) ◽  
pp. 2414-2423 ◽  
Author(s):  
Janine T. Bossé ◽  
Sunita Sinha ◽  
Ming-Shi Li ◽  
Clíona A. O'Dwyer ◽  
John H. E. Nash ◽  
...  
Keyword(s):  
Stress Response ◽  
Biofilm Formation ◽  
Serial Passage ◽  
Actinobacillus Pleuropneumoniae ◽  
Negative Regulator ◽  
Broth Culture ◽  
Solid Media ◽  
Transposon Mutants ◽  
Operon Expression ◽  
Repeated Passage

ABSTRACT Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-β-1,6-N-acetyl-d-glucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form biofilms on the surfaces of polystyrene plates, is lost following serial passage in broth culture, and repeated passage of the nonadherent variants on solid media does not result in reversion to the adherent colony phenotype. In order to investigate the regulation of PGA expression and biofilm formation in A. pleuropneumoniae, we screened a bank of transposon mutants of the nonadherent serovar 1 strain S4074T and identified mutations in two genes, rseA and hns, which resulted in the formation of the adherent colony phenotype. In other bacteria, including the Enterobacteriaceae, H-NS acts as a global gene regulator, and RseA is a negative regulator of the extracytoplasmic stress response sigma factor σE. Transcription profiling of A. pleuropneumoniae rseA and hns mutants revealed that both σE and H-NS independently regulate expression of the pga operon. Transcription of the pga operon is initiated from a σE promoter site in the absence of H-NS, and upregulation of σE is sufficient to displace H-NS, allowing transcription to proceed. In A. pleuropneumoniae, H-NS does not act as a global gene regulator but rather specifically regulates biofilm formation via repression of the pga operon. Positive regulation of the pga operon by σE indicates that biofilm formation is part of the extracytoplasmic stress response in A. pleuropneumoniae.

scholarly journals Poly-N-acetylglucosamine mediates biofilm formation and antibiotic resistance in Actinobacillus pleuropneumoniae

2007 ◽  
Vol 43 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Era A. Izano ◽  
Irina Sadovskaya ◽  
Evgeny Vinogradov ◽  
Martha H. Mulks ◽  
Kabilan Velliyagounder ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Biofilm Formation ◽  
Actinobacillus Pleuropneumoniae
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