Analysis on Actinobacillus pleuropneumoniae LuxS regulated genes reveals pleiotropic roles of LuxS/AI-2 on biofilm formation, adhesion ability and iron metabolism

2011 ◽  
Vol 50 (6) ◽  
pp. 293-302 ◽  
Author(s):  
Lu Li ◽  
Zhuofei Xu ◽  
Yang Zhou ◽  
Tingting Li ◽  
Lili Sun ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Biofilm Formation ◽  
Actinobacillus Pleuropneumoniae ◽  
Adhesion Ability

scholarly journals The ClpP Protease Is Required for the Stress Tolerance and Biofilm Formation in Actinobacillus pleuropneumoniae

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53600 ◽  
Author(s):  
Fang Xie ◽  
Yanhe Zhang ◽  
Gang Li ◽  
Long Zhou ◽  
Siguo Liu ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Biofilm Formation ◽  
Actinobacillus Pleuropneumoniae

scholarly journals Absence of TolC Impairs Biofilm Formation in Actinobacillus pleuropneumoniae by Reducing Initial Attachment

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0163364 ◽  
Author(s):  
Ying Li ◽  
Sanjie Cao ◽  
Luhua Zhang ◽  
Jianlin Yuan ◽  
Gee W. Lau ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Actinobacillus Pleuropneumoniae ◽  
Initial Attachment

Exposure to chlorine affects the extracellular polymeric substance production and cell surface hydrophobicity in biofilm bacteria

2012 ◽  
Vol 41 (4) ◽  
Author(s):  
Rengaswamy Rathi ◽  
Sathyaneson Satheesh
Keyword(s):  
Sodium Hypochlorite ◽  
Extracellular Polymeric Substance ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Strains ◽  
Adhesion Ability ◽  
Polymeric Substance ◽  
Test Organisms ◽  
Biofilm Bacteria

AbstractChlorination is a common antifouling method adopted by industrial units to minimize the fouling growth on cooling systems. In the present study, the effect of sodium hypochlorite on extracellular polymeric substance (EPS) production, hydrophobicity, cell adhesion and viability of marine bacteria involved in biofilm formation were assessed in laboratory condition. Two bacterial strains, tentatively identified as Alteromonas sp. and Pseudomonas sp. isolated from the surface of seaweeds were used as test organisms for the present study. The bacterial cultures were treated with sodium hypochlorite at 25% of the minimum inhibitory concentration. Results showed considerable variation in the production of EPS, viable counts, hydrophobicity and adhesion ability of bacteria treated with sodium hypochlorite. In general, the present study indicated that chlorination affects some important characteristics involved in the biofilm formation and thereby reduces the adhesion rate on surfaces.

scholarly journals Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σE and H-NS

2010 ◽  
Vol 192 (9) ◽  
pp. 2414-2423 ◽  
Author(s):  
Janine T. Bossé ◽  
Sunita Sinha ◽  
Ming-Shi Li ◽  
Clíona A. O'Dwyer ◽  
John H. E. Nash ◽  
...  
Keyword(s):  
Stress Response ◽  
Biofilm Formation ◽  
Serial Passage ◽  
Actinobacillus Pleuropneumoniae ◽  
Negative Regulator ◽  
Broth Culture ◽  
Solid Media ◽  
Transposon Mutants ◽  
Operon Expression ◽  
Repeated Passage

ABSTRACT Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-β-1,6-N-acetyl-d-glucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form biofilms on the surfaces of polystyrene plates, is lost following serial passage in broth culture, and repeated passage of the nonadherent variants on solid media does not result in reversion to the adherent colony phenotype. In order to investigate the regulation of PGA expression and biofilm formation in A. pleuropneumoniae, we screened a bank of transposon mutants of the nonadherent serovar 1 strain S4074T and identified mutations in two genes, rseA and hns, which resulted in the formation of the adherent colony phenotype. In other bacteria, including the Enterobacteriaceae, H-NS acts as a global gene regulator, and RseA is a negative regulator of the extracytoplasmic stress response sigma factor σE. Transcription profiling of A. pleuropneumoniae rseA and hns mutants revealed that both σE and H-NS independently regulate expression of the pga operon. Transcription of the pga operon is initiated from a σE promoter site in the absence of H-NS, and upregulation of σE is sufficient to displace H-NS, allowing transcription to proceed. In A. pleuropneumoniae, H-NS does not act as a global gene regulator but rather specifically regulates biofilm formation via repression of the pga operon. Positive regulation of the pga operon by σE indicates that biofilm formation is part of the extracytoplasmic stress response in A. pleuropneumoniae.

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