Molecular Epidemiology and Resistance Profile of Campylobacter jejuni and Campylobacter coli Strains Isolated from Different Sources in Brazil

2020 ◽  
Vol 26 (12) ◽  
pp. 1516-1525 ◽  
Author(s):  
Carolina N. Gomes ◽  
Miliane R. Frazão ◽  
Jaqueline Passaglia ◽  
Sheila S. Duque ◽  
Marta I. Cazentini Medeiros ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Campylobacter Jejuni ◽  
Campylobacter Coli ◽  
Resistance Profile ◽  
Different Sources

scholarly journals Molecular Epidemiology of Campylobacter jejuni Populations in Dairy Cattle, Wildlife, and the Environment in a Farmland Area

2008 ◽  
Vol 74 (16) ◽  
pp. 5130-5138 ◽  
Author(s):  
Patrick S. L. Kwan ◽  
Mishele Barrigas ◽  
Frederick J. Bolton ◽  
Nigel P. French ◽  
Peter Gowland ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Campylobacter Jejuni ◽  
Control Strategies ◽  
Horizontal Transmission ◽  
Environmental Water ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Different Sources ◽  
Sequence Types ◽  
Animal Reservoirs

ABSTRACT We describe a cross-sectional study of the molecular epidemiology of Campylobacter jejuni in a dairy farmland environment, with the aim of elucidating the dynamics of horizontal transmission of C. jejuni genotypes among sources in the area. A collection of 327 C. jejuni isolates from cattle, wildlife, and environmental sources in a 100-km2 area of farmland in northwest England was characterized by multilocus sequence typing. A total of 91 sequence types and 18 clonal complexes were identified. Clonal complexes ST-21, ST-45, and ST-61, which have been frequently associated with human disease, were the most commonly recovered genotypes in this study. In addition, widely distributed genotypes as well as potentially host-associated genotypes have been identified, which suggests that both restricted and interconnecting pathways of transmission may be operating in the dairy farmland environment. In particular, the ST-61 complex and the ST-21 complex were significantly associated with cattle. In contrast, complex strains ST-45, ST-952, and ST-677 were isolated predominantly from wild birds, wild rabbits, and environmental water. A considerable number of novel sequence types have also been identified, which were unassigned to existing clonal complexes and were frequently isolated from wildlife and environmental sources. The segregated distribution of genotypes among samples from different sources suggests that their transmission to humans is perhaps via independent routes. Insight into the dynamics and interactions of C. jejuni populations between important animal reservoirs and their surrounding environment would improve the identification of sources of Campylobacter infection and the design of control strategies.

Campylobacter coli bacteraemia: how common is it?

2020 ◽  
Vol 13 (12) ◽  
pp. e236634
Author(s):  
Sindhura Pisipati ◽  
Adnan Zafar ◽  
Yousaf Zafar
Keyword(s):  
Liver Cirrhosis ◽  
Campylobacter Jejuni ◽  
Unusual Case ◽  
Campylobacter Coli ◽  
Decompensated Liver Cirrhosis ◽  
The Past

Campylobacter species are known to cause enteritis. However, over the past 40–50 years, there have been reports of varying presentations, such as cellulitis, spondylodiscitis and bacteraemia. Of the Campylobacter species, Campylobacter jejuni is the most common culprit for causing bacteraemia, however, Campylobacter coli bacteraemia is becoming more prevalent. Here, we discuss an unusual case of C. coli bacteraemia in a patient with decompensated liver cirrhosis.

scholarly journals Whole genome-based characterisation of antimicrobial resistance and genetic diversity in Campylobacter jejuni and Campylobacter coli from ruminants

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Medelin Ocejo ◽  
Beatriz Oporto ◽  
José Luis Lavín ◽  
Ana Hurtado
Keyword(s):  
Genetic Diversity ◽  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Resistance Genes ◽  
Campylobacter Coli ◽  
Whole Genome ◽  
Northern Spain ◽  
Sequence Alignments ◽  
Phenotypic Data ◽  
Wide Range

AbstractCampylobacter, a leading cause of gastroenteritis in humans, asymptomatically colonises the intestinal tract of a wide range of animals.Although antimicrobial treatment is restricted to severe cases, the increase of antimicrobial resistance (AMR) is a concern. Considering the significant contribution of ruminants as reservoirs of resistant Campylobacter, Illumina whole-genome sequencing was used to characterise the mechanisms of AMR in Campylobacter jejuni and Campylobacter coli recovered from beef cattle, dairy cattle, and sheep in northern Spain. Genome analysis showed extensive genetic diversity that clearly separated both species. Resistance genotypes were identified by screening assembled sequences with BLASTn and ABRicate, and additional sequence alignments were performed to search for frameshift mutations and gene modifications. A high correlation was observed between phenotypic resistance to a given antimicrobial and the presence of the corresponding known resistance genes. Detailed sequence analysis allowed us to detect the recently described mosaic tet(O/M/O) gene in one C. coli, describe possible new alleles of blaOXA-61-like genes, and decipher the genetic context of aminoglycoside resistance genes, as well as the plasmid/chromosomal location of the different AMR genes and their implication for resistance spread. Updated resistance gene databases and detailed analysis of the matched open reading frames are needed to avoid errors when using WGS-based analysis pipelines for AMR detection in the absence of phenotypic data.

scholarly journals Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 594
Author(s):  
Sydney E. Schnur ◽  
Raghavendra G. Amachawadi ◽  
Giovanna Baca ◽  
Sarah Sexton-Bowser ◽  
Davina H. Rhodes ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Phenolic Compounds ◽  
Enterococcus Faecalis ◽  
Campylobacter Jejuni ◽  
Bacterial Pathogens ◽  
Klebsiella Oxytoca ◽  
Bovine Mastitis ◽  
Campylobacter Coli ◽  
Foodborne Illnesses ◽  
Phenolic Extract

Antimicrobial resistance in bacterial pathogens associated with bovine mastitis and human foodborne illnesses from contaminated food and water have an impact on animal and human health. Phenolic compounds have antimicrobial properties and some specialty sorghum grains are high in phenolic compounds, and the grain extract may have the potential as a natural antimicrobial alternative. The study’s objective was to determine antimicrobial effects of sorghum phenolic extract on bacterial pathogens that cause bovine mastitis and human foodborne illnesses. Bacterial pathogens tested included Escherichia coli, Salmonella Typhimurium, Campylobacter jejuni, Campylobacter coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Klebsiella oxytoca, Staphylococcus aureus, and Enterococcus faecalis. Antibacterial activities of sorghum phenolic extracts were determined by agar-well diffusion assay. Sorghum phenolic extract was added to the wells in concentrations of 0, 100, 200, 500, 1000, or 4000 µg/mL. The control wells did not receive phenolic extract. Plates were incubated for 18–24 h, and the diameter of each zone of inhibition was measured. The results indicated that sorghum phenolic extract had inhibitory effects on Staphylococcus aureus, Enterococcus faecalis, Campylobacter jejuni, and Campylobacter coli.

scholarly journals Distribution of CRISPR Types in Fluoroquinolone-Resistant Campylobacter jejuni Isolates

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 345
Author(s):  
Mehmet Cemal Adiguzel ◽  
Debora Brito Goulart ◽  
Zuowei Wu ◽  
Jinji Pang ◽  
Seyda Cengiz ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Campylobacter Jejuni ◽  
Phage Therapy ◽  
Point Mutations ◽  
Common Mutation ◽  
Gyra Gene ◽  
Nucleotide Homology ◽  
Different Sources

To aid development of phage therapy against Campylobacter, we investigated the distribution of the clustered regularly interspaced short palindromic repeats (CRISPR) systems in fluoroquinolone (FQ)-resistant Campylobacter jejuni. A total of 100 FQ-resistant C. jejuni strains from different sources were analyzed by PCR and DNA sequencing to determine resistance-conferring mutation in the gyrA gene and the presence of various CRISPR systems. All but one isolate harbored 1–5 point mutations in gyrA, and the most common mutation was the Thr86Ile change. Ninety-five isolates were positive with the CRISPR PCR, and spacer sequences were found in 86 of them. Among the 292 spacer sequences identified in this study, 204 shared 93–100% nucleotide homology to Campylobacter phage D10, 44 showed 100% homology to Campylobacter phage CP39, and 3 had 100% homology with Campylobacter phage CJIE4-5. The remaining 41 spacer sequences did not match with any phages in the database. Based on the results, it was inferred that the FQ-resistant C. jejuni isolates analyzed in this study were potentially resistant to Campylobacter phages D10, CP39, and CJIE4-5 as well as some unidentified phages. These phages should be excluded from cocktails of phages that may be utilized to treat FQ-resistant Campylobacter.

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