Characterization of VIM-4 Producing Clinical Pseudomonas aeruginosa Isolates from Western Algeria: Sequence Type and Class 1 Integron Description

2020 ◽  
Vol 26 (12) ◽  
pp. 1437-1441
Author(s):  
Fatma Zohra Zaidi ◽  
Radia Dali-Yahia ◽  
Karima Zenati ◽  
Leila Yazi ◽  
Manon Lounes ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Class 1 ◽  
Western Algeria

scholarly journals Characterization of the New Metallo-β-Lactamase VIM-13 and Its Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in Spain

2008 ◽  
Vol 52 (10) ◽  
pp. 3589-3596 ◽  
Author(s):  
Carlos Juan ◽  
Alejandro Beceiro ◽  
Olivia Gutiérrez ◽  
Sebastián Albertí ◽  
Margalida Garau ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pcr Amplification ◽  
Class 1 Integron ◽  
Negative Results ◽  
New Variant ◽  
Class B ◽  
Class 1 ◽  
Encoding Gene ◽  
Good Agreement

ABSTRACT During a survey conducted to evaluate the incidence of class B carbapenemase (metallo-β-lactamase [MBL])-producing Pseudomonas aeruginosa strains from hospitals in Majorca, Spain, five clinical isolates showed a positive Etest MBL screening test result. In one of them, strain PA-SL2, the presence of a new bla VIM derivative (bla VIM-13) was detected by PCR amplification with bla VIM-1-specific primers followed by sequencing. The bla VIM-13-producing isolate showed resistance to all β-lactams (except aztreonam), gentamicin, tobramycin, and ciprofloxacin. VIM-13 exhibited 93% and 88% amino acid sequence identities with VIM-1 and VIM-2, respectively. bla VIM-13 was cloned in parallel with bla VIM-1, and the resistance profile conferred was analyzed both in Escherichia coli and in P. aeruginosa backgrounds. Compared to VIM-1, VIM-13 conferred slightly higher levels of resistance to piperacillin and lower levels of resistance to ceftazidime and cefepime. VIM-13 and VIM-1 were purified in parallel as well, and their kinetic parameters were compared. The k cat/K m ratios for the antibiotics mentioned above were in good agreement with the MIC data. Furthermore, EDTA inhibited the activity of VIM-13 approximately 25 times less than it inhibited the activity of VIM-1. VIM-13 was harbored in a class 1 integron, along with a new variant (Ala108Thr) of the aminoglycoside-modifying enzyme encoding gene aacA4, which confers resistance to gentamicin and tobramycin. Finally, the VIM-13 integron was apparently located in the chromosome, since transformation and conjugation experiments consistently yielded negative results and the bla VIM-13 probe hybridized only with the genomic DNA.

scholarly journals Emergence of Pseudomonas aeruginosa with class 1 integron carrying blaVIM-2 and blaVIM-4 in the University Clinical Hospital of Bialystok (northeastern Poland)

2017 ◽  
Vol 71 (1) ◽  
pp. 0-0 ◽  
Author(s):  
Anna Michalska-Falkowska ◽  
Paweł Tomasz Sacha ◽  
Henryk Grześ ◽  
Tomasz Hauschild ◽  
Piotr Wieczorek ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Resistance ◽  
Genetic Relatedness ◽  
Housekeeping Genes ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Clinical Hospital ◽  
Class 1 ◽  
Severe Infections ◽  
The University

The effectiveness of carbapenems, considered as last-resort antimicrobials in severe infections, becomes compromised by bacterial resistance. The production of metallo-β-lactamases (MBLs) is the most significant threat to carbapenems activity among Pseudomonas aeruginosa. The aim of this study was to assess the presence and type of MBLs genes in carbapenem-resistant P. aeruginosa clinical strains, to identify the location of MBLs genes and to determine genetic relatedness between MBL-producers using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).The first identified MBL-positive (with blaVIM genes) P. aeruginosa strains were isolated from patients hospitalized in the University Clinical Hospital of Bialystok in the period from September 2012 to December 2013. Variants of MBLs genes and variable integron regions were characterized by PCR and sequencing. PFGE was performed after digesting of bacterial genomes by XbaI enzyme. By MLST seven housekeeping genes were analyzed for the determination of sequence type (ST). Three strains carried the blaVIM-2 gene and one harbored the blaVIM-4 gene. The blaVIM genes resided within class 1 integrons. PCR mapping of integrons revealed the presence of four different cassette arrays. Genetic relatedness analysis by PFGE classified VIM-positive strains into four unrelated pulsotypes (A–D). MLST demonstrated the presence of four (ST 111, ST27, and ST17) different sequence type including one previously undescribed new type of ST 2342. Antimicrobial susceptibility testing showed that VIM-positive strains were resistant to carbapenems, cephalosporins, aminoglycosides, and quinolones, intermediate to aztreonam, and susceptible only to colistin. Integrons mapping, PFGE, and MLST results may point to different origin of these strains and independent introduction into hospitalized patients.

scholarly journals Molecular Characterization ofblaNDM-1in a Sequence Type 235 Pseudomonas aeruginosa Isolate from France

2013 ◽  
Vol 57 (7) ◽  
pp. 3408-3411 ◽  
Author(s):  
Frédéric Janvier ◽  
Katy Jeannot ◽  
Sophie Tessé ◽  
Marjorie Robert-Nicoud ◽  
Hervé Delacour ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Insertion Sequence ◽  
Variable Region ◽  
Sequence Type ◽  
Common Region ◽  
Class 1 Integron ◽  
Genetic Studies ◽  
Content Type ◽  
Class 1 ◽  
Previous Hospitalization

ABSTRACTAn NDM-1 carbapenemase-producingPseudomonas aeruginosaisolate was recovered from a patient hospitalized in France after a previous hospitalization in Serbia. Genetic studies revealed that theblaNDM-1gene was surrounded by insertion sequence ISAba125and a truncated bleomycin resistance gene. ThisblaNDM-1region was a part of the variable region of a new complex class 1 integron bearing IS common region 1 (ISCR1). The presence of ISPa7upstream of this integron suggests insertion in a chromosomally located Tn402-like structure.

scholarly journals Characterization of Metallo-β-Lactamase VIM-27, an A57S Mutant of VIM-1 Associated with Klebsiella pneumoniae ST147

2011 ◽  
Vol 55 (7) ◽  
pp. 3570-3572 ◽  
Author(s):  
C. C. Papagiannitsis ◽  
S. D. Kotsakis ◽  
E. Petinaki ◽  
A. C. Vatopoulos ◽  
E. Tzelepi ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Kinetic Parameters ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1

ABSTRACTVIM-27 metallo-β-lactamase, an Ala57→ Ser variant of VIM-1, was identified in threeKlebsiella pneumoniaeisolates belonging to sequence type 147.blaVIM-27was part of a class 1 integron carried by non-self-transferable plasmids. Kinetic parameters and MIC determinations indicated that VIM-27 hydrolyzed most β-lactams, especially imipenem and cefoxitin, less effectively than VIM-1.

scholarly journals First Report ofblaIMP-14on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (8) ◽  
pp. 5068-5071 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert P. Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Multiple Drug Resistance ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Multiple Drug ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTheblaIMP-14carbapenem resistance gene has largely previously been observed inPseudomonas aeruginosaandAcinetobacterspp. As part of global surveillance and sequencing of carbapenem-resistantEscherichia coli, we identified a sequence type 131 strain harboringblaIMP-14within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C2plasmid. The emergence ofblaIMP-14in this context in the ST131 lineage is of potential clinical concern.

scholarly journals AAC(6′)-Iaf, a Novel Aminoglycoside 6′-N-Acetyltransferase from Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates

2009 ◽  
Vol 53 (6) ◽  
pp. 2327-2334 ◽  
Author(s):  
Tomoe Kitao ◽  
Tohru Miyoshi-Akiyama ◽  
Teruo Kirikae
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Decubitus Ulcer ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Upstream Region ◽  
Aminoglycoside Resistance ◽  
Class 1 Integron ◽  
Class 1 ◽  
Layer Chromatography

ABSTRACT We report here the characterization of a novel aminoglycoside resistance gene, aac(6′)-Iaf, present in two multidrug-resistant (MDR) Pseudomonas aeruginosa clinical isolates. These isolates, IMCJ798 and IMCJ799, were independently obtained from two patients, one with a urinary tract infection and the other with a decubitus ulcer, in a hospital located in the western part of Japan. Although the antibiotic resistance profiles of IMCJ798 and IMCJ799 were similar to that of MDR P. aeruginosa IMCJ2.S1, which caused outbreaks in the eastern part of Japan, the pulsed-field gel electrophoresis patterns for these isolates were different from that for IMCJ2.S1. Both IMCJ798 and IMCJ799 were found to contain a novel chromosomal class 1 integron, In123, which included aac(6′)-Iaf as the first cassette gene. The encoded protein, AAC(6′)-Iaf, was found to consist of 183 amino acids, with 91 and 87% identity to AAC(6′)-Iq and AAC(6′)-Im, respectively. IMCJ798, IMCJ799, and Escherichia coli transformants carrying a plasmid containing the aac(6′)-Iaf gene and its upstream region were highly resistant to amikacin, dibekacin, and kanamycin but not to gentamicin. The production of AAC(6′)-Iaf in these strains was confirmed by Western blot analysis. Thin-layer chromatography indicated that AAC(6′)-Iaf is a functional acetyltransferase that specifically modifies the amino groups at the 6′ positions of aminoglycosides. Collectively, these findings indicate that AAC(6′)-Iaf contributes to aminoglycoside resistance.

scholarly journals Dissemination of High-Risk Clones of Extensively Drug-Resistant Pseudomonas aeruginosa in Colombia

2015 ◽  
Vol 59 (4) ◽  
pp. 2421-2425 ◽  
Author(s):  
Adriana Correa ◽  
Rosa del Campo ◽  
Marcela Perenguez ◽  
Victor M. Blanco ◽  
Mercedes Rodríguez-Baños ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
High Risk ◽  
Sequence Type ◽  
Single Locus ◽  
Drug Resistant ◽  
Class 1 Integron ◽  
Content Type ◽  
Extensively Drug Resistant ◽  
Single Locus Variant ◽  
Class 1

ABSTRACTThe ability ofPseudomonas aeruginosato develop resistance to most antimicrobials represents an important clinical threat worldwide. We report the dissemination in several Colombian hospitals of two predominant lineages of extensively drug-resistant (XDR) carbapenemase-producingP. aeruginosastrains. These lineages belong to the high-risk clones sequence type 111 (ST111) and ST235 and harborblaVIM-2on a class 1 integron andblaKPC-2on a Tn4401transposon, respectively. Additionally,P. aeruginosaST1492, a novel single-locus variant of ST111, was identified. Clonal dissemination and the presence of mobile genetic elements likely explain the successful spread of XDRP. aeruginosastrains in Colombia.

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