Evaluation of Susceptibilities to Carbapenems and Faropenem Against Cephalosporin-Resistant Neisseria gonorrhoeae Clinical Isolates with penA Mosaic Alleles

2019 ◽  
Vol 25 (3) ◽  
pp. 427-433 ◽  
Author(s):  
Yoshiki Hiyama ◽  
Satoshi Takahashi ◽  
Toyotaka Sato ◽  
Masaaki Shinagawa ◽  
Yukari Fukushima ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Clinical Isolates

scholarly journals Mosaic-Like Structure of Penicillin-Binding Protein 2 Gene (penA) in Clinical Isolates of Neisseria gonorrhoeae with Reduced Susceptibility to Cefixime

2002 ◽  
Vol 46 (12) ◽  
pp. 3744-3749 ◽  
Author(s):  
Satoshi Ameyama ◽  
Shoichi Onodera ◽  
Masahiro Takahata ◽  
Shinzaburo Minami ◽  
Nobuko Maki ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Neisseria Meningitidis ◽  
Binding Protein ◽  
Clinical Isolates ◽  
Penicillin Binding Protein ◽  
Gene Sequences ◽  
Neisseria Flavescens ◽  
Neisseria Perflava

ABSTRACT Neisseria gonorrhoeae strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 μg/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, N. gonorrhoeae ATCC 19424, by transformation of the penicillin-binding protein 2 gene (penA) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 μg/ml). The sequences of penA in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported N. gonorrhoeae penA gene sequences. Some regions in the transpeptidase-encoding domain in this penA gene were similar to those in the penA genes of Neisseria perflava (N. sicca), Neisseria cinerea, Neisseria flavescens, and Neisseria meningitidis. These results showed that a mosaic-like structure in the penA gene conferred reductions in the levels of susceptibility of N. gonorrhoeae to cephems and penicillin in a manner similar to that found for N. meningitidis and Streptococcus pneumoniae.

Resistance, molecular characterization and viability of Neisseria gonorrhoeae recent clinical isolates

2021 ◽  
Vol 156 (5) ◽  
pp. 249-250
Author(s):  
Elizabeth Calatrava-Hernández ◽  
Carla Foronda-García-Hidalgo ◽  
José Gutiérrez-Fernández
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Molecular Characterization ◽  
Clinical Isolates

scholarly journals Amino Acid Substitutions in Mosaic Penicillin-Binding Protein 2 Associated with Reduced Susceptibility to Cefixime in Clinical Isolates of Neisseria gonorrhoeae

2006 ◽  
Vol 50 (11) ◽  
pp. 3638-3645 ◽  
Author(s):  
Sho Takahata ◽  
Nami Senju ◽  
Yumi Osaki ◽  
Takuji Yoshida ◽  
Takashi Ida
Keyword(s):  
Amino Acid ◽  
Neisseria Gonorrhoeae ◽  
Molecular Mechanisms ◽  
Binding Protein ◽  
Complete Sequence ◽  
Clinical Isolates ◽  
Amino Acid Substitutions ◽  
Penicillin Binding Protein ◽  
Fourfold Increase ◽  
Genes Encoding

ABSTRACT The molecular mechanisms of reduced susceptibility to cefixime in clinical isolates of Neisseria gonorrhoeae, particularly amino acid substitutions in mosaic penicillin-binding protein 2 (PBP2), were examined. The complete sequence of ponA, penA, and por genes, encoding, respectively, PBP1, PBP2, and porin, were determined for 58 strains isolated in 2002 from Japan. Replacement of leucine 421 by proline in PBP1 and the mosaic-like structure of PBP2 were detected in 48 strains (82.8%) and 28 strains (48.3%), respectively. The presence of mosaic PBP2 was the main cause of the elevated cefixime MIC (4- to 64-fold). In order to identify the mutations responsible for the reduced susceptibility to cefixime in isolates with mosaic PBP2, penA genes with various mutations were transferred to a susceptible strain by genetic transformation. The susceptibility of partial recombinants and site-directed mutants revealed that the replacement of glycine 545 by serine (G545S) was the primary mutation, which led to a two- to fourfold increase in resistance to cephems. Replacement of isoleucine 312 by methionine (I312M) and valine 316 by threonine (V316T), in the presence of the G545S mutation, reduced susceptibility to cefixime, ceftibuten, and cefpodoxime by an additional fourfold. Therefore, three mutations (G545S, I312M, and V316T) in mosaic PBP2 were identified as the amino acid substitutions responsible for reduced susceptibility to cefixime in N. gonorrhoeae.

scholarly journals DNA gyrase mutations in quinolone-resistant clinical isolates of Neisseria gonorrhoeae

1995 ◽  
Vol 39 (2) ◽  
pp. 561-563 ◽  
Author(s):  
T. Deguchi ◽  
M. Yasuda ◽  
M. Asano ◽  
K. Tada ◽  
H. Iwata ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Dna Gyrase ◽  
Clinical Isolates

scholarly journals Penicillinase-Producing Plasmid Types in Neisseria gonorrhoeae Clinical Isolates from Australia

2014 ◽  
Vol 58 (12) ◽  
pp. 7576-7578 ◽  
Author(s):  
David Whiley ◽  
Ella Trembizki ◽  
Cameron Buckley ◽  
Kevin Freeman ◽  
Andrew Lawrence ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Clinical Isolates ◽  
Content Type ◽  
Stepping Stone ◽  
Extended Spectrum

ABSTRACTPenicillinase-producingNeisseria gonorrhoeae(PPNG) carrying theblaTEM-135gene is of particular concern, as it is considered a stepping stone toward resistance to extended-spectrum cephalosporins. Here, we sought to characterize plasmid types and the occurrence of theblaTEM-135gene forN. gonorrhoeaeclinical isolates from Australia. We found thatblaTEM-135was prevalent in Australian PPNG and was detected on all three major plasmid types.

Confirmatory identification of Neisseria gonorrhoeae by slide coagglutination

1980 ◽  
Vol 26 (2) ◽  
pp. 218-222 ◽  
Author(s):  
Daniel V. Lim ◽  
Trevor Wall
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Clinical Isolates ◽  
Gram Stain ◽  
Carbohydrate Utilization ◽  
Valuable Alternative ◽  
Conventional Methods ◽  
Oxidase Reaction

Neisseria gonorrhoeae was identified by the Phadebact® gonococcus test, a rapid slide coagglutination technique, and the results obtained were compared with those obtained by conventional methods (Gram stain morphology, oxidase reaction, and carbohydrate utilization tests) for the confirmatory identification of gonococci. Of 308 clinical isolates examined, the coagglutination procedure correctly identified 97.8% of the isolates tested as N. gonorrhoeae and 93.9% of other bacteria as not N. gonorrhoeae. The coagglutination procedure also identified 29 laboratory strains correctly as not N. gonorrhoeae. The slide coagglutination test is easy to perform and offers a valuable alternative to other techniques for the confirmatory identification of N. gonorrhoeae.

scholarly journals Surveillance of antibiotic resistance in clinical isolates of Neisseria gonorrhoeae.

BMJ ◽  
1991 ◽  
Vol 303 (6813) ◽  
pp. 1307-1307 ◽  
Author(s):  
C A Ison ◽  
N S Branley ◽  
K Kirtland ◽  
C S Easmon
Keyword(s):  
Antibiotic Resistance ◽  
Neisseria Gonorrhoeae ◽  
Clinical Isolates

scholarly journals Identification of Drug Resistance Loci in Various Clinical Isolates of Neisseria gonorrhoeae

1977 ◽  
Vol 12 (3) ◽  
pp. 444-446 ◽  
Author(s):  
T. W. Maier ◽  
P. Warner ◽  
L. Zubryzycki ◽  
M. Chila
Keyword(s):  
Drug Resistance ◽  
Neisseria Gonorrhoeae ◽  
Clinical Isolates
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