scholarly journals Role of theN-Acetylmuramoyl-l-Alanyl Amidase, AmiA, ofHelicobacter pyloriin Peptidoglycan Metabolism, Daughter Cell Separation, and Virulence

2016 ◽  
Vol 22 (6) ◽  
pp. 477-486 ◽  
Author(s):  
Catherine Chaput ◽  
Chantal Ecobichon ◽  
Nadine Pouradier ◽  
Jean-Claude Rousselle ◽  
Abdelkader Namane ◽  
...  
Keyword(s):  
Cell Separation ◽  
Daughter Cell ◽  
Daughter Cell Separation

scholarly journals Structure and Functional Regulation of RipA, a Mycobacterial Enzyme Essential for Daughter Cell Separation

Structure ◽  
2010 ◽  
Vol 18 (9) ◽  
pp. 1184-1190 ◽  
Author(s):  
Alessia Ruggiero ◽  
Daniela Marasco ◽  
Flavia Squeglia ◽  
Silvia Soldini ◽  
Emilia Pedone ◽  
...  
Keyword(s):  
Cell Separation ◽  
Daughter Cell ◽  
Functional Regulation ◽  
Daughter Cell Separation

scholarly journals Mechanical crack propagation drives millisecond daughter cell separation in Staphylococcus aureus

Science ◽  
2015 ◽  
Vol 348 (6234) ◽  
pp. 574-578 ◽  
Author(s):  
X. Zhou ◽  
D. K. Halladin ◽  
E. R. Rojas ◽  
E. F. Koslover ◽  
T. K. Lee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Crack Propagation ◽  
Cell Separation ◽  
Daughter Cell ◽  
Mechanical Crack ◽  
Daughter Cell Separation

scholarly journals Fast Mechanically Driven Daughter Cell Separation Is Widespread in Actinobacteria

mBio ◽  
2016 ◽  
Vol 7 (4) ◽  
Author(s):  
Xiaoxue Zhou ◽  
David K. Halladin ◽  
Julie A. Theriot
Keyword(s):  
Cell Division ◽  
Cell Separation ◽  
Daughter Cell ◽  
Bacterial Species ◽  
Video Microscopy ◽  
Model Organisms ◽  
Gram Positive ◽  
Wide Range ◽  
Cell Shapes ◽  
Daughter Cell Separation

ABSTRACT Dividing cells of the coccoid Gram-positive bacterium Staphylococcus aureus undergo extremely rapid (millisecond) daughter cell separation (DCS) driven by mechanical crack propagation, a strategy that is very distinct from the gradual, enzymatically driven cell wall remodeling process that has been well described in several rod-shaped model bacteria. To determine if other bacteria, especially those in the same phylum ( Firmicutes ) or with similar coccoid shapes as S. aureus , might use a similar mechanically driven strategy for DCS, we used high-resolution video microscopy to examine cytokinesis in a phylogenetically wide range of species with various cell shapes and sizes. We found that fast mechanically driven DCS is rather rare in the Firmicutes (low G+C Gram positives), observed only in Staphylococcus and its closest coccoid relatives in the Macrococcus genus, and we did not observe this division strategy among the Gram-negative Proteobacteria . In contrast, several members of the high-G+C Gram-positive phylum Actinobacteria ( Micrococcus luteus , Brachybacterium faecium , Corynebacterium glutamicum , and Mycobacterium smegmatis ) with diverse shapes ranging from coccoid to rod all undergo fast mechanical DCS during cell division. Most intriguingly, similar fast mechanical DCS was also observed during the sporulation of the actinobacterium Streptomyces venezuelae . IMPORTANCE Much of our knowledge on bacterial cytokinesis comes from studying rod-shaped model organisms such as Escherichia coli and Bacillus subtilis . Less is known about variations in this process among different bacterial species. While cell division in many bacteria has been characterized to some extent genetically or biochemically, few species have been examined using video microscopy to uncover the kinetics of cytokinesis and daughter cell separation (DCS). In this work, we found that fast (millisecond) DCS is exhibited by species in two independent clades of Gram-positive bacteria and is particularly prevalent among the Actinobacteria , a diverse group that includes significant pathogens as well as bacteria that generate medically important antibiotics.

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