scholarly journals Anti-Inflammatory Effects of a Mixture of Lactic Acid Bacteria and Sodium Butyrate in Atopic Dermatitis Murine Model

2018 ◽  
Vol 21 (7) ◽  
pp. 716-725 ◽  
Author(s):  
Jeong A. Kim ◽  
Sung-Hak Kim ◽  
In Sung Kim ◽  
Da Yoon Yu ◽  
Sung Chan Kim ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Sodium Butyrate ◽  
Murine Model ◽  
Anti Inflammatory

scholarly journals Influence of Oral Administration of Lactic Acid Bacteria Metabolites on Skin Barrier Function and Water Content in a Murine Model of Atopic Dermatitis

2018 ◽  
Author(s):  
Yoshihiro Tokudome
Keyword(s):  
Atopic Dermatitis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Stratum Corneum ◽  
Water Content ◽  
Murine Model ◽  
Lipid Fraction ◽  
Transepidermal Water Loss ◽  
Water Soluble ◽  
Skin Barrier Function

The effects of orally administered lactic acid bacteria metabolites on the skin were studied using an atopic dermatitis-like murine model created by feeding mice with HR-AD. The lactic acid bacteria metabolites were obtained by inoculating 35 strains of 16 species of lactic acid bacteria into soy milk and culturing them. The atopic dermatitis-like murine model was created by feeding HR-1 mice HR-AD for 40 days. The skin condition of mice that were fed HR-AD worsened compared with normal mice, i.e., reduced water content in the stratum corneum, increased transepidermal water loss (TEWL), reduced ceramide AP content in the stratum corneum, and increased epidermis thickness. When mice that had been fed the HR-AD diet was administered a raw liquid of lactic acid bacteria metabolites orally, the measured values related to water content in the stratum corneum, TEWL, ceramide AP content in the stratum corneum, and epidermis thickness improved. To find out the active components for these effects, filtrate and residue from the raw liquid of lactic acid bacteria metabolites and lipid components extracted from the raw liquid were examined at the same time. Results showed that the water-soluble components or residue after filtration did not demonstrate effects but the raw liquid and the lipid fraction did. These findings suggest that lactic acid bacteria metabolites improve skin injury in an atopic dermatitis-like murine model.

scholarly journals Influence of Oral Administration of Lactic Acid Bacteria Metabolites on Skin Barrier Function and Water Content in a Murine Model of Atopic Dermatitis

Nutrients ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1858 ◽  
Author(s):  
Yoshihiro Tokudome
Keyword(s):  
Atopic Dermatitis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Stratum Corneum ◽  
Water Content ◽  
Murine Model ◽  
Lipid Fraction ◽  
Transepidermal Water Loss ◽  
Water Soluble ◽  
Skin Barrier Function

The effects of orally administered lactic acid bacteria metabolites on skin were studied using an atopic dermatitis-like murine model generated by feeding HR-AD to mice. Lactic acid bacteria metabolites were obtained by inoculating and culturing soy milk with 35 strains of 16 species of lactic acid bacteria. The atopic dermatitis-like murine model was generated by feeding HR-AD to HR-1 mice for 40 days. The skin condition of HR-AD-fed mice worsened compared with normal mice, showing reduced water content in the stratum corneum, increased transepidermal water loss (TEWL), reduced ceramide AP content in the stratum corneum, and increased epidermis thickness. When HR-AD-fed mice were orally administered a raw liquid containing lactic acid bacteria metabolites, water content in the stratum corneum, TEWL, ceramide AP content in the stratum corneum, and epidermis thickness improved. To determine the active components responsible for these effects, filtrate, residue, and lipid components extracted from the raw liquid containing lactic acid bacteria metabolites were examined. While water-soluble components and residue obtained after filtration had no effects, the lipid fraction showed similar effects to the raw liquid. These findings suggest that lactic acid bacteria metabolites improve skin injury in an atopic dermatitis-like murine model.

scholarly journals Dectin-2 mediates phagocytosis of Lactobacillus paracasei KW3110 and IL-10 production by macrophages

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mia Yoshikawa ◽  
Sayuri Yamada ◽  
Miho Sugamata ◽  
Osamu Kanauchi ◽  
Yuji Morita
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Interleukin 10 ◽  
Lactobacillus Paracasei ◽  
Binding Affinities ◽  
Antibody Treatment ◽  
Lectin Array ◽  
Specific Strain ◽  
Anti Inflammatory ◽  
Carbohydrate Chains

AbstractLactic acid bacteria (LAB) are most generally used as probiotics and some strains of LAB are known to have anti-inflammatory effects. A specific strain of lactic acid bacteria, Lactobacillus paracasei KW3110 (KW3110), activates macrophages to produce interleukin-10 (IL-10), an anti-inflammatory cytokine; however, the biological mechanism remains unclear. In this study, we showed that the amount of incorporated KW3110 into a macrophage cell line, RAW 264.7, was higher than other genetically related strains using fluorescence microscopy. RNA-seq analysis indicated that treatment of macrophages with KW3110 induced Dectin-2 gene expression, which is a pattern recognition receptor, recognizing α-mannose. In addition, antibody treatment and knock down of Dectin-2, or factors downstream in the signaling pathway, decreased the amount of incorporated KW3110 and IL-10 production. Substantial lectin array analysis also revealed that KW3110 had higher binding affinities to lectins, which recognize the carbohydrate chains comprised of α-mannose, than two other LAB. In conclusion, KW3110 is readily incorporated into macrophages, leading to IL-10 production. Dectin-2 mediated the phagocytosis of KW3110 into macrophages and this may be involved with the characteristic carbohydrate chains of KW3110.

scholarly journals Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitis

2017 ◽  
Vol 95 (9) ◽  
pp. 1005-1015 ◽  
Author(s):  
Mab Pereira Corrêa ◽  
Frans Eberth Costa Andrade ◽  
Alexandre Dantas Gimenes ◽  
Cristiane Damas Gil
Keyword(s):  
Atopic Dermatitis ◽  
Murine Model ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Targeted Screening of Lactic Acid Bacteria With Antibacterial Activity Toward Staphylococcus aureus Clonal Complex Type 1 Associated With Atopic Dermatitis

2021 ◽  
Vol 12 ◽  
Author(s):  
Ida B. Christensen ◽  
Charlotte Vedel ◽  
Maja-Lisa Clausen ◽  
Søren Kjærulff ◽  
Tove Agner ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Atopic Dermatitis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Organic Acids ◽  
Clonal Complex ◽  
Treatment Strategies ◽  
Complex Type

Atopic dermatitis (AD) is a common inflammatory skin disease characterized by an epidermal barrier impairment, as well as a Th2/Th22-skewed immune response, both favoring skin colonization with Staphylococcus aureus. Colonization is strongly related to severity of the disease, and a reduction of S. aureus has been found to alleviate symptoms. Lactic acid bacteria (LAB) produce antimicrobial compounds such as organic acids and bacteriocins and are widely used as probiotics. The aim of this study was to isolate LAB and screen for antibacterial effect specifically toward S. aureus clonal complex type 1. A total of 680 LAB were isolated from fermented vegetables and swab samples from healthy volunteers (vaginal, stool and skin). Screening for antibacterial activity toward S. aureus, narrowed the field of isolates down to four LAB strains with high antibacterial activity. The activity varied according to the specific LAB strain and the origin of the strain. The results suggested different modes of action, including co-aggregation, expression of bacteriocins and production of specific organic acids. However, the ability to acidify the surroundings appeared as the main effect behind inhibition of S. aureus. Broth microdilution assays showed a significant reduction of S. aureus growth when using down to 10% cell free supernatant (CFS). Our results underline the use of specific living LAB or their CFS as potential future treatment strategies to reduce S. aureus colonization of AD skin.

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