scholarly journals Pediatric and Teen Ovarian Tissue Removed for Cryopreservation Contains Follicles Irrespective of Age, Disease Diagnosis, Treatment History, and Specimen Processing Methods

2015 ◽  
Vol 4 (4) ◽  
pp. 174-183 ◽  
Author(s):  
Francesca E. Duncan ◽  
Mary Ellen Pavone ◽  
Alexander H. Gunn ◽  
Sherif Badawy ◽  
Clarisa Gracia ◽  
...  
Keyword(s):  
Ovarian Tissue ◽  
Disease Diagnosis ◽  
Processing Methods ◽  
Treatment History ◽  
Specimen Processing

scholarly journals Vaginal swab specimen processing methods influence performance of rapid semen detection tests: a cautionary tale

Contraception ◽  
2010 ◽  
Vol 82 (3) ◽  
pp. 291-295 ◽  
Author(s):  
Marcia M. Hobbs ◽  
Markus J. Steiner ◽  
Kimberly D. Rich ◽  
Maria F. Gallo ◽  
Lee Warner ◽  
...  
Keyword(s):  
Vaginal Swab ◽  
Cautionary Tale ◽  
Processing Methods ◽  
Specimen Processing ◽  
Semen Detection ◽  
Swab Specimen

scholarly journals Laboratory comparison of stool processing methods for Xpert® Ultra

2021 ◽  
Vol 11 (2) ◽  
pp. 55-57
Author(s):  
C. L. Jasumback ◽  
Q. Dlamini ◽  
J. Kahari ◽  
G. Maphalala ◽  
M. G. Dlamini ◽  
...  
Keyword(s):  
Error Rate ◽  
Healthy Adult ◽  
Disease Diagnosis ◽  
Invasive Procedures ◽  
Bacille Calmette Guerin ◽  
Processing Methods ◽  
Specific Symptoms ◽  
Relative Detection

TB disease diagnosis in children is difficult due to non-specific symptoms, paucibacillary disease and the need for invasive procedures to obtain diagnostic specimens. In many settings, these specimens are simply not collected and therefore stool, easily obtained, has emerged as a promising specimen for the diagnosis of child TB. In this study, stool from a healthy adult was spiked with known concentrations of bacille Calmette-Guérin vaccine and tested using the Xpert® Ultra assay to determine the relative detection and error rate associated with four different published stool processing methods.

scholarly journals Arrays of Arrays for High-Throughput Gene Expression Profiling

2001 ◽  
Vol 11 (7) ◽  
pp. 1256-1261
Author(s):  
Patrick P. Zarrinkar ◽  
James K. Mainquist ◽  
Matthew Zamora ◽  
David Stern ◽  
John B. Welsh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Expression Profiles ◽  
Ovarian Tissue ◽  
Disease Diagnosis ◽  
Full Potential ◽  
Ovarian Carcinomas ◽  
Single Experiment ◽  
Tissue Samples

Gene expression profiling using DNA arrays is rapidly becoming an essential tool for research and drug discovery and may soon play a central role in disease diagnosis. Although it is possible to make significant discoveries on the basis of a relatively small number of expression profiles, the full potential of this technology is best realized through more extensive collections of expression measurements. The generation of large numbers of expression profiles can be a time-consuming and labor-intensive process with current one-at-a-time technology. We have developed the ability to obtain expression profiles in a highly parallel yet straightforward format using glass wafers that contain 49 individual high-density oligonucleotide arrays. This arrays of arrays concept is generalizable and can be adapted readily to other types of arrays, including spotted cDNA microarrays. It is also scalable for use with hundreds and even thousands of smaller arrays on a single piece of glass. Using the arrays of arrays approach and parallel preparation of hybridization samples in 96-well plates, we were able to determine the patterns of gene expression in 27 ovarian carcinomas and 4 normal ovarian tissue samples, along with a number of control samples, in a single experiment. This new approach significantly increases the ease, efficiency, and throughput of microarray-based experiments and makes possible new applications of expression profiling that are currently impractical.

Use of TEM in Plant Disease Diagnosis: A Xylem-Limited Bacterium Associated with Diseased ‘Toronto’ Creeping Bentgrass

1981 ◽  
Vol 39 ◽  
pp. 414-415
Author(s):  
Karen K. Baker ◽  
David L. Roberts
Keyword(s):  
Osmium Tetroxide ◽  
Plant Disease ◽  
Leaf Tissue ◽  
Infectious Agent ◽  
Creeping Bentgrass ◽  
Disease Diagnosis ◽  
Unknown Etiology ◽  
Agrostis Palustris ◽  
Putting Greens ◽  
Plant Disease Diagnosis

Plant disease diagnosis is most often accomplished by examination of symptoms and observation or isolation of causal organisms. Occasionally, diseases of unknown etiology occur and are difficult or impossible to accurately diagnose by the usual means. In 1980, such a disease was observed on Agrostis palustris Huds. c.v. Toronto (creeping bentgrass) putting greens at the Butler National Golf Course in Oak Brook, IL.The wilting symptoms of the disease and the irregular nature of its spread through affected areas suggested that an infectious agent was involved. However, normal isolation procedures did not yield any organism known to infect turf grass. TEM was employed in order to aid in the possible diagnosis of the disease.Crown, root and leaf tissue of both infected and symptomless plants were fixed in cold 5% glutaraldehyde in 0.1 M phosphate buffer, post-fixed in buffered 1% osmium tetroxide, dehydrated in ethanol and embedded in a 1:1 mixture of Spurrs and epon-araldite epoxy resins.

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