Alkaline Peroxide Pretreatment of Corn Stover for Enzymatic Saccharification and Ethanol Production

2014 ◽  
Vol 10 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Badal C. Saha ◽  
Michael A. Cotta
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Enzymatic Saccharification ◽  
Alkaline Peroxide ◽  
Alkaline Peroxide Pretreatment

scholarly journals The Potential of Cellulosic Ethanol Production from Grasses in Thailand

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Jinaporn Wongwatanapaiboon ◽  
Kunn Kangvansaichol ◽  
Vorakan Burapatana ◽  
Ratanavalee Inochanon ◽  
Pakorn Winayanuwattikun ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Ethanol Production ◽  
Cellulosic Ethanol ◽  
Alternative Energy ◽  
Maximum Yield ◽  
Enzymatic Saccharification ◽  
Pichia Stipitis ◽  
Vetiver Grass ◽  
Average Percentage ◽  
Alkaline Peroxide

The grasses in Thailand were analyzed for the potentiality as the alternative energy crops for cellulosic ethanol production by biological process. The average percentage composition of cellulose, hemicellulose, and lignin in the samples of 18 types of grasses from various provinces was determined as 31.85–38.51, 31.13–42.61, and 3.10–5.64, respectively. The samples were initially pretreated with alkaline peroxide followed by enzymatic hydrolysis to investigate the enzymatic saccharification. The total reducing sugars in most grasses ranging from 500–600 mg/g grasses (70–80% yield) were obtained. Subsequently, 11 types of grasses were selected as feedstocks for the ethanol production by simultaneous saccharification and cofermentation (SSCF). The enzymes, cellulase and xylanase, were utilized for hydrolysis and the yeasts,Saccharomyces cerevisiaeandPichia stipitis,were applied for cofermentation at 35°C for 7 days. From the results, the highest yield of ethanol, 1.14 g/L or 0.14 g/g substrate equivalent to 32.72% of the theoretical values was obtained from Sri Lanka ecotype vetiver grass. When the yields of dry matter were included in the calculations, Sri Lanka ecotype vetiver grass gave the yield of ethanol at 1,091.84 L/ha/year, whereas the leaves of dwarf napier grass showed the maximum yield of 2,720.55 L/ha/year (0.98 g/L or 0.12 g/g substrate equivalent to 30.60% of the theoretical values).

scholarly journals Alkaline peroxide pretreatment of corn stover: effects of biomass, peroxide, and enzyme loading and composition on yields of glucose and xylose

2011 ◽  
Vol 4 (1) ◽  
pp. 16 ◽  
Author(s):  
Goutami Banerjee ◽  
Suzana Car ◽  
John S Scott-Craig ◽  
David B Hodge ◽  
Jonathan D Walton
Keyword(s):  
Corn Stover ◽  
Enzyme Loading ◽  
Alkaline Peroxide ◽  
Alkaline Peroxide Pretreatment

scholarly journals Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw

Energies ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 4574 ◽  
Author(s):  
Seung Hyeon Park ◽  
Thi Thu Huong Pham ◽  
Tae Hyun Kim
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Inoculum Size ◽  
Lignocellulosic Material ◽  
Pretreated Corn Stover ◽  
Ethanol Yields ◽  
Saccharification Yield ◽  
Ammonia Method

Synergistic effect of cellulase and hemicellulase (xylanase) was evaluated because lignocellulosic material is a heterogeneous complex of cellulose and hemicellulose. Various effects of HTec2 addition on enzymatic saccharification and fermentation were evaluated using two different substrates such as corn stover and rice straw. Corn stover and rice straw were pretreated by the LMAA (low-moisture anhydrous ammonia) method at the preselected same conditions (90 °C, 120 h, moisture content = 50%, NH3 loading = 0.1 g NH3/g). It was observed that the enzymatic saccharification yield of pretreated corn stover (76.4% for glucan digestibility) was higher than that of pretreated rice straw (70.9% for glucan) using CTec2 cellulase without HTec2 addition. Glucan digestibility of pretreated corn stover was significantly increased from 76.4% to 91.1% when the HTec2/CTec2 (v/v) increased from 0 to 10. However, it was interesting that the ethanol production was decreased from 89.9% to 76.3% for SSF and 118.0% to 87.9% for SSCF at higher HTec2/CTec2. As the glucan loading increased from 2.0% to 7.0%, the ethanol yields of both SSF and SSCF were decreased from 96.3% to 88.9% and from 116.6% to 92.4%, respectively. In addition, the smallest inoculum size (optical density of 0.25) resulted in the highest ethanol production (20.5 g/L).

Alkaline peroxide pretreatment for efficient enzymatic saccharification of bamboo

2010 ◽  
Vol 79 (4) ◽  
pp. 914-920 ◽  
Author(s):  
Yuya Yamashita ◽  
Megumi Shono ◽  
Chizuru Sasaki ◽  
Yoshitoshi Nakamura
Keyword(s):  
Enzymatic Saccharification ◽  
Alkaline Peroxide ◽  
Alkaline Peroxide Pretreatment

scholarly journals Comparison of Corn Stover Pretreatments with Lewis Acid Catalyzed Choline Chloride, Glycerol and Choline Chloride-Glycerol Deep Eutectic Solvent

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1170
Author(s):  
Yuan Zhu ◽  
Benkun Qi ◽  
Xinquan Liang ◽  
Jianquan Luo ◽  
Yinhua Wan
Keyword(s):  
Corn Stover ◽  
Lewis Acid ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Enzymatic Saccharification ◽  
Glycerol Solution ◽  
Cellulose Conversion ◽  
Biomass Processing ◽  
Acid Catalyzed ◽  
Highly Correlated

Herein, corn stover (CS) was pretreated by less corrosive lewis acid FeCl3 acidified solutions of neat and aqueous deep eutectic solvent (DES), aqueous ChCl and glycerol at 120 °C for 4 h with single FeCl3 pretreatment as control. It was unexpected that acidified solutions of both ChCl and glycerol were found to be more efficient at removing lignin and xylan, leading to higher enzymatic digestibility of pretreated CS than acidified DES. Comparatively, acidified ChCl solution exhibited better pretreatment performance than acidified glycerol solution. In addition, 20 wt% water in DES dramatically reduced the capability of DES for delignification and xylan removal and subsequent enzymatic cellulose saccharification of pretreated CS. Correlation analysis showed that enzymatic saccharification of pretreated CS was highly correlated to delignification and cellulose crystallinity, but lowly correlated to xylan removal. Recyclability experiments of different acidified pretreatment solutions showed progressive decrease in the pretreatment performance with increasing recycling runs. After four cycles, the smallest decrease in enzymatic cellulose conversion (22.07%) was observed from acidified neat DES pretreatment, while the largest decrease (43.80%) was from acidified ChCl pretreatment. Those findings would provide useful information for biomass processing with ChCl, glycerol and ChCl-glycerol DES.

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