scholarly journals Monoclonal Antibodies Against LipL32, The Major Outer Membrane Protein of PathogenicLeptospira: Production, Characterization, and Testing in Diagnostic Applications

Hybridoma ◽  
2007 ◽  
Vol 26 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Cláudia P.H. Fernandes ◽  
Fabiana K. Seixas ◽  
Mariana L. Coutinho ◽  
Flávia A. Vasconcellos ◽  
Núbia Seyffert ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Major Outer Membrane Protein ◽  
Diagnostic Applications

scholarly journals Antigenic Analysis of Avian Chlamydia psittaci using Monoclonal Antibodies to the Major Outer Membrane Protein.

1991 ◽  
Vol 53 (3) ◽  
pp. 385-389 ◽  
Author(s):  
Atsushi KIKUTA ◽  
Nahotaka FURUKAWA ◽  
Tetsuya YOSHIDA ◽  
Hideto FUKUSHI ◽  
Tsuyoshi YAMAGUCHI ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Major Outer Membrane Protein ◽  
Chlamydia Psittaci ◽  
Antigenic Analysis

scholarly journals Protection of Wild-Type and Severe Combined Immunodeficiency Mice against an Intranasal Challenge by Passive Immunization with Monoclonal Antibodies to the Chlamydia trachomatis Mouse Pneumonitis Major Outer Membrane Protein

2008 ◽  
Vol 76 (12) ◽  
pp. 5581-5587 ◽  
Author(s):  
Sukumar Pal ◽  
Jose Bravo ◽  
Ellena M. Peterson ◽  
Luis M. de la Maza
Keyword(s):  
Body Weight ◽  
Monoclonal Antibodies ◽  
Chlamydia Trachomatis ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Passive Immunization ◽  
Major Outer Membrane Protein ◽  
Wild Type

ABSTRACT Monoclonal antibodies (MAbs) to the Chlamydia trachomatis mouse pneumonitis (MoPn) major outer membrane protein (MOMP) were characterized for their ability to neutralize the infectivity of this organism in vitro and in vivo. One of the MAbs (MoPn-23) recognizes a nonlinear epitope in the MOMP, MAb MoPn-40 binds to a linear epitope in the variable domain 1 (VD1), and MAb MoPn-32 recognizes the chlamydial lipopolysaccharide. MAb MoPn-23 neutralized 50% of the infectivity of Chlamydia, as measured in vitro by using HAK (FcγIII−) and HeLa-229 (FcγIII+) cells at a concentration 100 times lower than MAb MoPn-40. MAb MoPn-32 had no neutralizing ability. In comparison to the control normal mouse immunoglobulin G, passive immunization of BALB/c mice with MAb MoPn-23 resulted in a highly significant protection against an intranasal (i.n.) challenge as determined by the change in body weight, the weight of the lungs, and the yield of Chlamydia inclusion-forming units (IFU) from the lungs. Passive immunization with MAb MoPn-40 resulted in a lower degree of protection, and MAb MoPn-32 afforded no protection. MAb MoPn-23 was also tested for its ability to protect wild-type (WT) and severe combined immunodeficient (SCID) C.B-17 mice against an i.n. challenge. Protection based on total body weight, lung weight, and yield of Chlamydia IFU was as effective in SCID as in WT C.B-17 mice. In conclusion, antibodies to MOMP can protect mice against a chlamydial infection in the presence or absence of T and B cells.

Sign in / Sign up

Export Citation Format

Share Document