The engineered MARCH8-resistant VSV glycoprotein enhances lentiviral vector transduction

2021 ◽  
Author(s):  
Fengwen Xu ◽  
Xiaoman Liu ◽  
Di Zhang ◽  
Fei Zhao ◽  
Zhangling Fan ◽  
...  
Keyword(s):  
Lentiviral Vector ◽  
Vector Transduction

scholarly journals Abrogation of postentry restriction of HIV-1-based lentiviral vector transduction in simian cells

2003 ◽  
Vol 100 (3) ◽  
pp. 1298-1303 ◽  
Author(s):  
N. A. Kootstra ◽  
C. Munk ◽  
N. Tonnu ◽  
N. R. Landau ◽  
I. M. Verma
Keyword(s):  
Lentiviral Vector ◽  
Hiv 1 ◽  
Vector Transduction

scholarly journals ZNF521 Represses Osteoblastic Differentiation in Human Adipose-Derived Stem Cells

2018 ◽  
Vol 19 (12) ◽  
pp. 4095 ◽  
Author(s):  
Emanuela Chiarella ◽  
Annamaria Aloisio ◽  
Stefania Scicchitano ◽  
Valeria Lucchino ◽  
Ylenia Montalcini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Zinc Finger Protein ◽  
Lentiviral Vector ◽  
Adipogenic Differentiation ◽  
Bone Matrix ◽  
Osteoblastic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Matrix Mineralization ◽  
Calcium Deposits ◽  
Vector Transduction

Human adipose-derived stem cells (hADSCs) are multipotent mesenchymal cells that can differentiate into adipocytes, chondrocytes, and osteocytes. During osteoblastogenesis, the osteoprogenitor cells differentiate into mature osteoblasts and synthesize bone matrix components. Zinc finger protein 521 (ZNF521/Zfp521) is a transcription co-factor implicated in the regulation of hematopoietic, neural, and mesenchymal stem cells, where it has been shown to inhibit adipogenic differentiation. The present study is aimed at determining the effects of ZNF521 on the osteoblastic differentiation of hADSCs to clarify whether it can influence their osteogenic commitment. The enforced expression or silencing of ZNF521 in hADSCs was achieved by lentiviral vector transduction. Cells were cultured in a commercial osteogenic medium for up to 20 days. The ZNF521 enforced expression significantly reduced osteoblast development as assessed by the morphological and molecular criteria, resulting in reduced levels of collagen I, alkaline phosphatase, osterix, osteopontin, and calcium deposits. Conversely, ZNF521 silencing, in response to osteoblastic stimuli, induced a significant increase in early molecular markers of osteogenesis and, at later stages, a remarkable enhancement of matrix mineralization. Together with our previous findings, these results show that ZNF521 inhibits both adipocytic and osteoblastic maturation in hADSCs and suggest that its expression may contribute to maintaining the immature properties of hADSCs.

scholarly journals Lentiviral vector transduction of spermatozoa as a tool for the study of early development

FEBS Open Bio ◽  
2014 ◽  
Vol 4 (1) ◽  
pp. 266-275 ◽  
Author(s):  
Anil Chandrashekran ◽  
Ihsan Isa ◽  
Jayesh Dudhia ◽  
Adrian J. Thrasher ◽  
Nicholas Dibb ◽  
...  
Keyword(s):  
Early Development ◽  
Lentiviral Vector ◽  
Vector Transduction

scholarly journals Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation

2009 ◽  
Vol 37 (19) ◽  
pp. 6587-6599 ◽  
Author(s):  
Rachael Kenworthy ◽  
Diana Lambert ◽  
Feng Yang ◽  
Nan Wang ◽  
Zihong Chen ◽  
...  
Keyword(s):  
Lentiviral Vector ◽  
Short Hairpin ◽  
Short Hairpin Rnas ◽  
Vector Transduction

scholarly journals Prolonged transgene expression in murine salivary glands following non-primate lentiviral vector transduction

2005 ◽  
Vol 12 (1) ◽  
pp. 137-143 ◽  
Author(s):  
Ela Shai ◽  
Aaron Palmon ◽  
Amos Panet ◽  
Yitzhak Marmary ◽  
Yoav Sherman ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Transgene Expression ◽  
Lentiviral Vector ◽  
Vector Transduction
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