scholarly journals Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation

2009 ◽  
Vol 37 (19) ◽  
pp. 6587-6599 ◽  
Author(s):  
Rachael Kenworthy ◽  
Diana Lambert ◽  
Feng Yang ◽  
Nan Wang ◽  
Zihong Chen ◽  
...  
Keyword(s):  
Lentiviral Vector ◽  
Short Hairpin ◽  
Short Hairpin Rnas ◽  
Vector Transduction

scholarly journals Multiple gene knock-down by a single lentiviral vector expressing an array of short hairpin RNAs

2006 ◽  
Vol 9 (5) ◽  
pp. 0-0 ◽  
Author(s):  
Veronique Stove ◽  
Kaatje Smits ◽  
Evelien Naessens ◽  
Jean Plum ◽  
Bruno Verhasselt
Keyword(s):  
Lentiviral Vector ◽  
Multiple Gene ◽  
Knock Down ◽  
Short Hairpin ◽  
Short Hairpin Rnas

scholarly journals Large-Scale, High-Throughput Validation of Short Hairpin RNA Sequences for RNA Interference

2006 ◽  
Vol 11 (3) ◽  
pp. 236-246 ◽  
Author(s):  
Laurence H. Lamarcq ◽  
Bradley J. Scherer ◽  
Michael L. Phelan ◽  
Nikolai N. Kalnine ◽  
Yen H. Nguyen ◽  
...  
Keyword(s):  
High Throughput ◽  
Large Scale ◽  
Strong Support ◽  
Gc Content ◽  
Rapid Identification ◽  
Hairpin Rna ◽  
Rna Sequences ◽  
Short Hairpin ◽  
Short Hairpin Rnas ◽  
Interfering Rna

A method for high-throughput cloning and analysis of short hairpin RNAs (shRNAs) is described. Using this approach, 464 shRNAs against 116 different genes were screened for knockdown efficacy, enabling rapid identification of effective shRNAs against 74 genes. Statistical analysis of the effects of various criteria on the activity of the shRNAs confirmed that some of the rules thought to govern small interfering RNA (siRNA) activity also apply to shRNAs. These include moderate GC content, absence of internal hairpins, and asymmetric thermal stability. However, the authors did not find strong support for positionspecific rules. In addition, analysis of the data suggests that not all genes are equally susceptible to RNAinterference (RNAi).

scholarly journals Abrogation of postentry restriction of HIV-1-based lentiviral vector transduction in simian cells

2003 ◽  
Vol 100 (3) ◽  
pp. 1298-1303 ◽  
Author(s):  
N. A. Kootstra ◽  
C. Munk ◽  
N. Tonnu ◽  
N. R. Landau ◽  
I. M. Verma
Keyword(s):  
Lentiviral Vector ◽  
Hiv 1 ◽  
Vector Transduction

scholarly journals Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yang Zhang ◽  
Tuan M. Nguyen ◽  
Xiao-Ou Zhang ◽  
Limei Wang ◽  
Tin Phan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
High Throughput ◽  
Biological Effect ◽  
Human Hepatocellular Carcinoma ◽  
Guide Rnas ◽  
Rna Targeting ◽  
Short Hairpin ◽  
Short Hairpin Rnas ◽  
Screening Library ◽  
High Throughput Study

AbstractShort hairpin RNAs (shRNAs) are used to deplete circRNAs by targeting back-splicing junction (BSJ) sites. However, frequent discrepancies exist between shRNA-mediated circRNA knockdown and the corresponding biological effect, querying their robustness. By leveraging CRISPR/Cas13d tool and optimizing the strategy for designing single-guide RNAs against circRNA BSJ sites, we markedly enhance specificity of circRNA silencing. This specificity is validated in parallel screenings by shRNA and CRISPR/Cas13d libraries. Using a CRISPR/Cas13d screening library targeting > 2500 human hepatocellular carcinoma-related circRNAs, we subsequently identify a subset of sorafenib-resistant circRNAs. Thus, CRISPR/Cas13d represents an effective approach for high-throughput study of functional circRNAs.

Lentivirus vectors expressing short hairpin RNAs against the U3-overlapping region of HIV nef inhibit HIV replication and infectivity in primary macrophages

Blood ◽  
2006 ◽  
Vol 108 (10) ◽  
pp. 3305-3312 ◽  
Author(s):  
T. Yamamoto ◽  
H. Miyoshi ◽  
N. Yamamoto ◽  
N. Yamamoto ◽  
J.-i. Inoue ◽  
...  
Keyword(s):  
Hiv Replication ◽  
Short Hairpin ◽  
Lentivirus Vectors ◽  
Short Hairpin Rnas ◽  
Overlapping Region

scholarly journals ZNF521 Represses Osteoblastic Differentiation in Human Adipose-Derived Stem Cells

2018 ◽  
Vol 19 (12) ◽  
pp. 4095 ◽  
Author(s):  
Emanuela Chiarella ◽  
Annamaria Aloisio ◽  
Stefania Scicchitano ◽  
Valeria Lucchino ◽  
Ylenia Montalcini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Zinc Finger Protein ◽  
Lentiviral Vector ◽  
Adipogenic Differentiation ◽  
Bone Matrix ◽  
Osteoblastic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Matrix Mineralization ◽  
Calcium Deposits ◽  
Vector Transduction

Human adipose-derived stem cells (hADSCs) are multipotent mesenchymal cells that can differentiate into adipocytes, chondrocytes, and osteocytes. During osteoblastogenesis, the osteoprogenitor cells differentiate into mature osteoblasts and synthesize bone matrix components. Zinc finger protein 521 (ZNF521/Zfp521) is a transcription co-factor implicated in the regulation of hematopoietic, neural, and mesenchymal stem cells, where it has been shown to inhibit adipogenic differentiation. The present study is aimed at determining the effects of ZNF521 on the osteoblastic differentiation of hADSCs to clarify whether it can influence their osteogenic commitment. The enforced expression or silencing of ZNF521 in hADSCs was achieved by lentiviral vector transduction. Cells were cultured in a commercial osteogenic medium for up to 20 days. The ZNF521 enforced expression significantly reduced osteoblast development as assessed by the morphological and molecular criteria, resulting in reduced levels of collagen I, alkaline phosphatase, osterix, osteopontin, and calcium deposits. Conversely, ZNF521 silencing, in response to osteoblastic stimuli, induced a significant increase in early molecular markers of osteogenesis and, at later stages, a remarkable enhancement of matrix mineralization. Together with our previous findings, these results show that ZNF521 inhibits both adipocytic and osteoblastic maturation in hADSCs and suggest that its expression may contribute to maintaining the immature properties of hADSCs.

Lentiviral vector delivery of short hairpin RNA to NG2 and neurotrophin-3 promotes locomotor recovery in injured rat spinal cord

Cytotherapy ◽  
2012 ◽  
Vol 14 (10) ◽  
pp. 1235-1244 ◽  
Author(s):  
Eleanor M. Donnelly ◽  
Nicolas N. Madigan ◽  
Gemma E. Rooney ◽  
Andrew Knight ◽  
Bingkun Chen ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Lentiviral Vector ◽  
Short Hairpin Rna ◽  
Rat Spinal Cord ◽  
Hairpin Rna ◽  
Locomotor Recovery ◽  
Neurotrophin 3 ◽  
Short Hairpin ◽  
Vector Delivery

A quick and efficient approach for gene silencing by using triple putative microRNA-based short hairpin RNAs

2008 ◽  
Vol 323 (1-2) ◽  
pp. 81-89 ◽  
Author(s):  
Z. X. Shan ◽  
Q. X. Lin ◽  
M. Yang ◽  
C. Y. Deng ◽  
S. J. Kuang ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Efficient Approach ◽  
Short Hairpin ◽  
Short Hairpin Rnas
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