Bone marrow mesenchymal stem cells-derived exosomal microRNA-150-3p promotes osteoblast proliferation and differentiation in osteoporosis

2020 ◽  
Author(s):  
Min Qiu ◽  
Shuheng Zhai ◽  
Qin Fu ◽  
Da Liu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteoblast Proliferation ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Exosomal Microrna

scholarly journals Levels of matrix metalloproteinase-2 in committed differentiation of bone marrow mesenchymal stem cells induced by kartogenin

2019 ◽  
Vol 47 (7) ◽  
pp. 3261-3270
Author(s):  
Cheng Wang ◽  
Qiaohui Liu ◽  
Xiaoyuan Ma ◽  
Guofeng Dai
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Matrix Metalloproteinase ◽  
Type Ii Collagen ◽  
Matrix Metalloproteinase 2 ◽  
Type Ii ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells

Objective To measure the inductive effect of kartogenin on matrix metalloproteinase-2 levels during the differentiation of human bone marrow mesenchymal stem cells (hMSCs) into chondrocytes in vitro. Methods In vitro cultured bone marrow hMSCs were grown to the logarithmic phase and then divided into three groups: control group (0 µM kartogenin), 1 µM kartogenin group and 10 µM kartogenin group. After 72 h of culture, cell proliferation and differentiation were observed microscopically. Matrix metalloproteinase-2 (MMP-2) in the cell supernatant and type II collagen levels in the cells were detected by enzyme linked immunosorbent assay and immunofluorescence staining, respectively. Results Kartogenin induced the proliferation and differentiation of hMSCs. With the increase of kartogenin concentration, the level of type II collagen was increased, while the level of MMP-2 decreased. Conclusion These findings indicate that kartogenin can induce hMSCs to differentiate into chondrocytes, and with the increase of kartogenin concentration, degeneration of the cartilage extracellular matrix may be inhibited.

Bone Marrow Mesenchymal Stem Cells-Derived Exosomes Promote Osteoporosis and Osteoblast Proliferation by Inhibiting Bax/Bcl-2/Caspase Signaling Pathway

2020 ◽  
Vol 10 (3) ◽  
pp. 418-423
Author(s):  
Shengxing Liu ◽  
Kaizhong Zhu ◽  
Hao Qi ◽  
Hai Zhao ◽  
Dingzhong Chen
Keyword(s):  
Bone Mineral Density ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Signaling Pathway ◽  
Volume Fraction ◽  
Serum Osteocalcin ◽  
Osteoblast Proliferation ◽  
Mineral Density ◽  
Marrow Mesenchymal Stem Cells

The current treatment for osteoporosis cannot restore the lost bone mass. Exosomes can not only delay bone loss, but also promote osteogenic proliferation and differentiation. This study aims to determine whether BMSCs-derived exosomes could improve osteoporosis in rats. A rat model of osteoporosis was established by excising bilateral ovaries. Whole bone marrow cell inoculation method and adherent purification were used to culture rat bone marrow mesenchymal stem cells (BMSCs). Osteoporosis rats were treated with BMSCs-derived exosomes followed by analysis of serum osteocalcin level by ELISA and bone mineral density and volume fraction by Micro-CT. Osteoblasts were treated with exosomes followed by analysis of cell proliferation by CCK-8 assay and expression of key molecules in the apoptotic signaling by real-time PCR and Western blot. Serum osteocalcin, bone mineral density and volume fraction in OVX group were significantly lower than those in control group and Exo group (P < 0.05) with significant differences between Exo group and CON group. With the increase of exosome concentration, the survival rate of osteoblasts was increased gradually. Cleaved Caspase-3 expression was reduced and Bcl-2 level was elevated gradually. BMSCs-derived exosomes can promote osteoblast proliferation by inhibiting apoptotic signaling pathway and improving osteoporosis.

scholarly journals Bone Marrow Mesenchymal Stem Cells Stimulate Cardiac Stem Cell Proliferation and Differentiation

2010 ◽  
Vol 107 (7) ◽  
pp. 913-922 ◽  
Author(s):  
Konstantinos E. Hatzistergos ◽  
Henry Quevedo ◽  
Behzad N. Oskouei ◽  
Qinghua Hu ◽  
Gary S. Feigenbaum ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cardiac Stem Cell ◽  
Stem Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells
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