scholarly journals Lentiviral Vectors Mediate Efficient and Stable Gene Transfer in Adult Neural Stem Cells In Vivo

2006 ◽  
Vol 0 (0) ◽  
pp. 060801084750007
Author(s):  
Martine Geraerts ◽  
Kristel Eggermont ◽  
Pilar Hernandez-Acosta ◽  
Jose-Manuel Garcia-Verdugo ◽  
Veerle Baekelandt ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Transfer ◽  
Neural Stem Cells ◽  
Lentiviral Vectors ◽  
Stable Gene ◽  
Adult Neural Stem Cells

scholarly journals Robust in vivo gene transfer into adult mammalian neural stem cells by lentiviral vectors

2004 ◽  
Vol 101 (41) ◽  
pp. 14835-14840 ◽  
Author(s):  
A. Consiglio ◽  
A. Gritti ◽  
D. Dolcetta ◽  
A. Follenzi ◽  
C. Bordignon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Transfer ◽  
Neural Stem Cells ◽  
Lentiviral Vectors ◽  
In Vivo Gene Transfer

scholarly journals In Vivo Fate Mapping and Expression Analysis Reveals Molecular Hallmarks of Prospectively Isolated Adult Neural Stem Cells

2011 ◽  
Vol 8 (1) ◽  
pp. 119
Author(s):  
Ruth Beckervordersandforth ◽  
Pratibha Tripathi ◽  
Jovica Ninkovic ◽  
Efil Bayam ◽  
Alexandra Lepier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Expression Analysis ◽  
Fate Mapping ◽  
Adult Neural Stem Cells

Phenotypic change of newly generated neurons from adult neural stem cells in vivo

2009 ◽  
Vol 65 ◽  
pp. S101-S102
Author(s):  
Tetsuji Mori ◽  
Taketoshi Wakabayashi ◽  
Yasuharu Takamori ◽  
Kotaro Kitaya ◽  
Hisao Yamada
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Phenotypic Change ◽  
Adult Neural Stem Cells

scholarly journals Single-cell in vivo imaging of adult neural stem cells in the zebrafish telencephalon

2016 ◽  
Vol 11 (8) ◽  
pp. 1360-1370 ◽  
Author(s):  
Joana S Barbosa ◽  
Rossella Di Giaimo ◽  
Magdalena Götz ◽  
Jovica Ninkovic
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Single Cell ◽  
In Vivo Imaging ◽  
Adult Neural Stem Cells

scholarly journals Robust In Vivo Transduction of Nervous System and Neural Stem Cells by Early Gestational Intra Amniotic Gene Transfer Using Lentiviral Vector

2010 ◽  
Vol 18 (9) ◽  
pp. 1615-1623 ◽  
Author(s):  
David H Stitelman ◽  
Masayuki Endo ◽  
Archana Bora ◽  
Nidal Muvarak ◽  
Philip W Zoltick ◽  
...  
Keyword(s):  
Stem Cells ◽  
Nervous System ◽  
Gene Transfer ◽  
Neural Stem Cells ◽  
Lentiviral Vector

Dynamic effects of Fto in regulating the proliferation and differentiation of adult neural stem cells of mice

2019 ◽  
Vol 29 (5) ◽  
pp. 727-735 ◽  
Author(s):  
Yuhang Cao ◽  
Yingliang Zhuang ◽  
Junchen Chen ◽  
Weize Xu ◽  
Yikai Shou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Neuronal Development ◽  
Conditional Knockout ◽  
Biological Processes ◽  
Adult Neural Stem Cells ◽  
Proliferation And Differentiation

Abstract N 6-methyladenosine (m6A) modification of RNA is deposited by the methyltransferase complex consisting of Mettl3 and Mettl14 and erased by demethylase Fto and Alkbh5 and is involved in diverse biological processes. However, it remains largely unknown the specific function and mechanism of Fto in regulating adult neural stem cells (aNSCs). In the present study, utilizing a conditional knockout (cKO) mouse model, we show that the specific ablation of Fto in aNSCs transiently increases the proliferation of aNSCs and promotes neuronal differentiation both in vitro and in vivo, but in a long term, the specific ablation of Fto inhibits adult neurogenesis and neuronal development. Mechanistically, Fto deficiency results in a significant increase in m6A modification in Pdgfra and Socs5. The increased expression of Pdgfra and decreased expression of Socs5 synergistically promote the phosphorylation of Stat3. The modulation of Pdgfra and Socs5 can rescue the neurogenic deficits induced by Fto depletion. Our results together reveal an important function of Fto in regulating aNSCs through modulating Pdgfra/Socs5-Stat3 pathway.

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