scholarly journals Single-cell in vivo imaging of adult neural stem cells in the zebrafish telencephalon

2016 ◽  
Vol 11 (8) ◽  
pp. 1360-1370 ◽  
Author(s):  
Joana S Barbosa ◽  
Rossella Di Giaimo ◽  
Magdalena Götz ◽  
Jovica Ninkovic
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Single Cell ◽  
In Vivo Imaging ◽  
Adult Neural Stem Cells

scholarly journals In Vivo Fate Mapping and Expression Analysis Reveals Molecular Hallmarks of Prospectively Isolated Adult Neural Stem Cells

2011 ◽  
Vol 8 (1) ◽  
pp. 119
Author(s):  
Ruth Beckervordersandforth ◽  
Pratibha Tripathi ◽  
Jovica Ninkovic ◽  
Efil Bayam ◽  
Alexandra Lepier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Expression Analysis ◽  
Fate Mapping ◽  
Adult Neural Stem Cells

Phenotypic change of newly generated neurons from adult neural stem cells in vivo

2009 ◽  
Vol 65 ◽  
pp. S101-S102
Author(s):  
Tetsuji Mori ◽  
Taketoshi Wakabayashi ◽  
Yasuharu Takamori ◽  
Kotaro Kitaya ◽  
Hisao Yamada
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Phenotypic Change ◽  
Adult Neural Stem Cells

scholarly journals In vivo live imaging of postnatal neural stem cells

Development ◽  
2021 ◽  
Vol 148 (18) ◽  
Author(s):  
Alina Marymonchyk ◽  
Sarah Malvaut ◽  
Armen Saghatelyan
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vivo Imaging ◽  
Live Imaging ◽  
Future Directions ◽  
Environmental Signals ◽  
New Information ◽  
Imaging Results

ABSTRACT Neural stem cells (NSCs) are maintained in specific regions of the postnatal brain and contribute to its structural and functional plasticity. However, the long-term renewal potential of NSCs and their mode of division remain elusive. The use of advanced in vivo live imaging approaches may expand our knowledge of NSC physiology and provide new information for cell replacement therapies. In this Review, we discuss the in vivo imaging methods used to study NSC dynamics and recent live-imaging results with respect to specific intracellular pathways that allow NSCs to integrate and decode different micro-environmental signals. Lastly, we discuss future directions that may provide answers to unresolved questions regarding NSC physiology.

Dynamic effects of Fto in regulating the proliferation and differentiation of adult neural stem cells of mice

2019 ◽  
Vol 29 (5) ◽  
pp. 727-735 ◽  
Author(s):  
Yuhang Cao ◽  
Yingliang Zhuang ◽  
Junchen Chen ◽  
Weize Xu ◽  
Yikai Shou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Neuronal Development ◽  
Conditional Knockout ◽  
Biological Processes ◽  
Adult Neural Stem Cells ◽  
Proliferation And Differentiation

Abstract N 6-methyladenosine (m6A) modification of RNA is deposited by the methyltransferase complex consisting of Mettl3 and Mettl14 and erased by demethylase Fto and Alkbh5 and is involved in diverse biological processes. However, it remains largely unknown the specific function and mechanism of Fto in regulating adult neural stem cells (aNSCs). In the present study, utilizing a conditional knockout (cKO) mouse model, we show that the specific ablation of Fto in aNSCs transiently increases the proliferation of aNSCs and promotes neuronal differentiation both in vitro and in vivo, but in a long term, the specific ablation of Fto inhibits adult neurogenesis and neuronal development. Mechanistically, Fto deficiency results in a significant increase in m6A modification in Pdgfra and Socs5. The increased expression of Pdgfra and decreased expression of Socs5 synergistically promote the phosphorylation of Stat3. The modulation of Pdgfra and Socs5 can rescue the neurogenic deficits induced by Fto depletion. Our results together reveal an important function of Fto in regulating aNSCs through modulating Pdgfra/Socs5-Stat3 pathway.

scholarly journals LRIG1 is a gatekeeper to exit from quiescence in adult neural stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
María Ángeles Marqués-Torrejón ◽  
Charles A. C. Williams ◽  
Benjamin Southgate ◽  
Neza Alfazema ◽  
Melanie P. Clements ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Single Cell Analysis ◽  
Interferon Response ◽  
Quiescent State ◽  
Adult Neural Stem Cells ◽  
Protein Levels

AbstractAdult neural stem cells (NSCs) must tightly regulate quiescence and proliferation. Single-cell analysis has suggested a continuum of cell states as NSCs exit quiescence. Here we capture and characterize in vitro primed quiescent NSCs and identify LRIG1 as an important regulator. We show that BMP-4 signaling induces a dormant non-cycling quiescent state (d-qNSCs), whereas combined BMP-4/FGF-2 signaling induces a distinct primed quiescent state poised for cell cycle re-entry. Primed quiescent NSCs (p-qNSCs) are defined by high levels of LRIG1 and CD9, as well as an interferon response signature, and can efficiently engraft into the adult subventricular zone (SVZ) niche. Genetic disruption of Lrig1 in vivo within the SVZ NSCs leads an enhanced proliferation. Mechanistically, LRIG1 primes quiescent NSCs for cell cycle re-entry and EGFR responsiveness by enabling EGFR protein levels to increase but limiting signaling activation. LRIG1 is therefore an important functional regulator of NSC exit from quiescence.

scholarly journals Integration of Genome-wide Approaches Identifies lncRNAs of Adult Neural Stem Cells and Their Progeny In Vivo

2013 ◽  
Vol 12 (5) ◽  
pp. 616-628 ◽  
Author(s):  
Alexander D. Ramos ◽  
Aaron Diaz ◽  
Abhinav Nellore ◽  
Ryan N. Delgado ◽  
Ki-Youb Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Adult Neural Stem Cells ◽  
Genome Wide
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