scholarly journals Efficient Inhibition of Human Telomerase Reverse Transcriptase Expression by RNA Interference Sensitizes Cancer Cells to Ionizing Radiation and Chemotherapy

2005 ◽  
Vol 16 (7) ◽  
pp. 859-868 ◽  
Author(s):  
Mitsuhiro Nakamura ◽  
Kenkichi Masutomi ◽  
Satoru Kyo ◽  
Manabu Hashimoto ◽  
Yoshiko Maida ◽  
...  
Keyword(s):  
Rna Interference ◽  
Ionizing Radiation ◽  
Reverse Transcriptase ◽  
Cancer Cells ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Human Telomerase

scholarly journals RNA interference mediated downregulation of human telomerase reverse transcriptase (hTERT) in LN18 cells

2016 ◽  
Vol 68 (6) ◽  
pp. 2311-2321 ◽  
Author(s):  
Ch. Lavanya ◽  
M. K. Sibin ◽  
M. M. Srinivas Bharath ◽  
M. Jeru Manoj ◽  
Manjunatha M. Venkataswamy ◽  
...  
Keyword(s):  
Rna Interference ◽  
Reverse Transcriptase ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Human Telomerase

Selective Apoptosis and Growth Impairment of Cancer Cells Induced by Human Telomerase Reverse Transcriptase (hTERT) Targeting Artificial MicroRNA

2021 ◽  
Vol 13 (9) ◽  
pp. 1644-1656
Author(s):  
Hongjun Lin ◽  
Pengliang Xin ◽  
Huangen Li ◽  
Mingqing Tang
Keyword(s):  
Reverse Transcriptase ◽  
Cancer Cells ◽  
Telomerase Activity ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Reverse Transcription Pcr ◽  
Angiogenesis Assay ◽  
Amplification Protocol ◽  
Huvec Cells ◽  
Human Telomerase

Human telomerase reverse transcriptase (hTERT) is a promising cancer target, and amiRNA particle displays the siRNA’s specificity and miRNA’s safety, suggesting that cancers can be treated more effective and safely by hTERT targeting amiRNA particles. Hela, NCI-H446, U2-OS and Huvec cells were transfected by hTERT targeting amiRNA particles. hTERT expression, telomerase activity and cell viability were evaluated by quantitative reverse transcription-PCR (qRT-PCR), western blot (WB), telomeric repeat amplification protocol (TRAP) assays, MTT method, transwell protocol, fluorescence-activated cell sorting (FACS) technologies, angiogenesis assay, and xenograft tumor models. Results: hTERT expression and telomerase activity in Hela and NCIH446 were significantly inhibited by amiRNA. Anti-proliferation and pro-apoptosis effects were only observed in transfected Hela and NCI-H446 cells, but anti-migration and anti-angiogenesis effects were presented in transfected Huvec cells. More interestingly, low to 1.56 nM amiRNA can inhibit the proliferation of Hela cells by 80.99±5.24%. Conclusion: amiRNA selectively and effectively impairs the growth, and assists the apoptosis of telomerase-positive cancer cells.

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