TGF‐β‐mediated regulation of plasminogen activators is human telomerase reverse transcriptase dependent in cancer cells

BioFactors ◽  
2019 ◽  
Vol 45 (5) ◽  
pp. 803-817 ◽  
Author(s):  
Rishi K. Jaiswal ◽  
Pramod K. Yadava
Keyword(s):  
Reverse Transcriptase ◽  
Cancer Cells ◽  
Plasminogen Activators ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Human Telomerase

Selective Apoptosis and Growth Impairment of Cancer Cells Induced by Human Telomerase Reverse Transcriptase (hTERT) Targeting Artificial MicroRNA

2021 ◽  
Vol 13 (9) ◽  
pp. 1644-1656
Author(s):  
Hongjun Lin ◽  
Pengliang Xin ◽  
Huangen Li ◽  
Mingqing Tang
Keyword(s):  
Reverse Transcriptase ◽  
Cancer Cells ◽  
Telomerase Activity ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Reverse Transcription Pcr ◽  
Angiogenesis Assay ◽  
Amplification Protocol ◽  
Huvec Cells ◽  
Human Telomerase

Human telomerase reverse transcriptase (hTERT) is a promising cancer target, and amiRNA particle displays the siRNA’s specificity and miRNA’s safety, suggesting that cancers can be treated more effective and safely by hTERT targeting amiRNA particles. Hela, NCI-H446, U2-OS and Huvec cells were transfected by hTERT targeting amiRNA particles. hTERT expression, telomerase activity and cell viability were evaluated by quantitative reverse transcription-PCR (qRT-PCR), western blot (WB), telomeric repeat amplification protocol (TRAP) assays, MTT method, transwell protocol, fluorescence-activated cell sorting (FACS) technologies, angiogenesis assay, and xenograft tumor models. Results: hTERT expression and telomerase activity in Hela and NCIH446 were significantly inhibited by amiRNA. Anti-proliferation and pro-apoptosis effects were only observed in transfected Hela and NCI-H446 cells, but anti-migration and anti-angiogenesis effects were presented in transfected Huvec cells. More interestingly, low to 1.56 nM amiRNA can inhibit the proliferation of Hela cells by 80.99±5.24%. Conclusion: amiRNA selectively and effectively impairs the growth, and assists the apoptosis of telomerase-positive cancer cells.

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