Cell Line Identity Finding by Fingerprinting, an Optimized Resource for Short Tandem Repeat Profile Authentication

2013 ◽  
Vol 17 (3) ◽  
pp. 254-259 ◽  
Author(s):  
Alessio Somaschini ◽  
Nadia Amboldi ◽  
Angelo Nuzzo ◽  
Emanuela Scacheri ◽  
Giorgio Ukmar ◽  
...  
Keyword(s):  
Cell Line ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Cell Line Identity ◽  
Short Tandem Repeat Profile ◽  
Short Tandem

scholarly journals Research Resource: STR DNA Profile and Gene Expression Comparisons of Human BG-1 Cells and a BG-1/MCF-7 Clonal Variant

2014 ◽  
Vol 28 (12) ◽  
pp. 2072-2081 ◽  
Author(s):  
Yin Li ◽  
Yukitomo Arao ◽  
Julie M. Hall ◽  
Sandra Burkett ◽  
Liwen Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ovarian Cancer ◽  
Cell Line ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Expression Patterns ◽  
Genotypic Differences ◽  
Dna Profile ◽  
Short Tandem ◽  
Mcf 7

Human ovarian cancer BG-1 cells are a valuable in vitro model that has enabled several laboratories to study the estrogenic responses of ovarian cancers. We recently discovered that there are two different BG-1 cell lines being used for experiments, denoted here as BG-1 FR and BG-1 NIEHS, which exhibit striking morphological differences. The objective of this study was to methodically analyze these two BG-1 variants and compare their characteristics. Short tandem repeat analysis revealed that the DNA profile of BG-1 FR cells was unique, yet the Short tandem repeat pattern of BG-1 NIEHS was identical with that of MCF-7 cells. From a cytogenetic analysis, it became apparent that the BG-1 FR line had the same profile as previously reported, whereas the BG-1 NIEHS and MCF-7 cells share a similar genetic display. A significant number of unique chromosomal translocations were observed between the BG-1 NIEHS and MCF-7 cells, suggesting that acquired genotypic differences resulted in the formation of two lines from a common origin. Although all cell types demonstrated a similar estrogen responsiveness in reporter gene assays, a microarray analysis revealed distinct estrogen-responsive gene expression patterns with surprisingly moderate to low overlap. We conclude that BG-1 FR is the original ovarian cancer cell line, whereas the BG-1 NIEHS is a variant from the MCF-7 cells. These findings provide much needed clarification of the identities and characteristics of key cell line models that are widely used to study estrogen action in female reproductive cancers.

Cancer cell line identification by short tandem repeat profiling: power and limitations

2005 ◽  
Vol 19 (3) ◽  
pp. 1-18 ◽  
Author(s):  
Walther Parson ◽  
Romana Kirchebner ◽  
Roswitha Mühlmann ◽  
Kathrin Renner ◽  
Anita Kofler ◽  
...  
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Cancer Cell Line ◽  
Line Identification ◽  
Cell Line Identification ◽  
Short Tandem Repeat Profiling ◽  
Short Tandem

scholarly journals Mixture Patterned Short Tandem Repeat Profile in a Perimortem Transfused Patient

2016 ◽  
Vol 40 (1) ◽  
pp. 27
Author(s):  
Hee Jin Seo ◽  
Sohee Cho ◽  
Ji Hyun Lee ◽  
Sung Hee Lyoo ◽  
Sohyung Park ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Short Tandem Repeat Profile ◽  
Short Tandem

scholarly journals Feasibility of single-cell analysis of model cancer and foetal cells in blood after isolation by cell picking

Tumor Biology ◽  
2019 ◽  
Vol 41 (2) ◽  
pp. 101042831882336 ◽  
Author(s):  
Julie Smith ◽  
Andreas Frøslev Mathisen ◽  
Nadja Funch Richardt ◽  
Ann-Sophie Vander Plaetsen ◽  
Filip Van Nieuwerburgh ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cell Line ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Target Cells ◽  
Chain Reaction ◽  
Blood Samples ◽  
Polymerase Chain ◽  
Single Tumour ◽  
Short Tandem

The objective of the present feasibility study was to transfer single cell line cells to either microscopy slides for downstream immune characterization or to polymerase chain reaction tubes for downstream DNA quantitation. Tumour cell lines, SKBR3 and MCF7 and trophoblast cell line JEG-3 were spiked in healthy donor blood. The CytoTrack system was used to scan the spiked blood samples to identify target cells. Individual target cells were identified, picked by use of a CytoPicker and deposited to either a microscopic slide or a polymerase chain reaction tube (PCR). Single tumour cells on microscopic slides were further immunostained with human epidermal growth factor receptor 2 (Her2) and epithelial cell adhesion molecule (EpCAM). From the picked cells in polymerase chain reaction tubes, DNA was amplified, quantified and used for Short Tandem Repeat genotyping. Depositing rare cells to microscopy slides was laborious with only five cells per hour. In this study with a trained operator, the picked cells had an 80.5% recovery rate. Depositing single trophoblast cells in PCR tubes was a faster process with 10 cells in 5 min. Immunostaining of isolated cells by both Her2 and EpCAM was possible but showed varying staining intensity. Presence of trophoblasts and contaminating white blood cells in PCR tubes after cell picking was confirmed based on DNA yield and mixed Short Tandem Repeat profiles in five out of eight samples. Using the CytoPicker tool, single tumour and trophoblast cells were successfully isolated and moved from blood samples, allowing subsequent immunostaining or Short Tandem Repeat genotyping.

scholarly journals The Use of Short Tandem Repeat Analysis for Cell Line Authentication

2019 ◽  
Vol 19 (4) ◽  
pp. 251-260
Author(s):  
M. D. Khorolsky ◽  
I. S. Semenova ◽  
E. V. Melnikova ◽  
Yu. V. Olefir
Keyword(s):  
Quality Control ◽  
Cell Line ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Genetic Stability ◽  
Routine Practice ◽  
Stability Testing ◽  
Cell Line Authentication ◽  
Repeat Analysis ◽  
Short Tandem

Short tandem repeat analysis (STR) is a well-established international method of authentication and genetic stability testing of cell lines (CLs). Therefore, the development and introduction of this method into routine practice of cell banks and cell culture collections is a pressing concern. In addition, the expansion of the field of cell-line based biomedical cell products (BСPs) necessitates the implementation of STR as a tool of identification testing during quality control. The State Pharmacopoeia of the Russian Federation does not require mandatory use of STR for cell line identification, while other countries have been using this method for cell line quality control for about a decade. The use of identified CLs in medical practice will ensure the efficacy and safety of BCPs.The aim of the study was to assess the possibility of using STR analysis for authentication and genetic stability testing of CLs using U937, WISH, WIL2-S, NK-92, and Jurkat Clone E6-1 CLs as examples.Materials and me­thods: the following human CLs were used in the study: U937 (ECACC), WISH (ATCC), WIL2S (ATCC), NK-92 (ATCC), and Jurkat Clone E6-1 (ATCC). The CL allelic profiles were determined by STR using the COrDIS Plus kit (Gordiz, Russia). The electrophoretic separation was performed using a Genetic Analyzer 3500 Series instrument. The data provided on the websites of the European Collection of Authenticated Cell Cultures and American Type Culture Collection were used to compare the CL profiles.Results: the AuthentiFiler PCR Amplification Kit (Thermo Fisher Scientific, USA) and the GenePrint 10 System (Promega Corporation, USA) intended for CL authentication by STR were compared with the characteristics of the COrDIS plus kit (Gordiz, Russia). The results of the comparison demonstrated that the COrDIS plus kit includes all the loci found in the foreign kits, as well as the loci recommended by the International Cell Line Authentication Committee. The U-937, WIL2S, and NK-92 CLs demonstrated genetic identity with the reference profiles available on the websites of the international collections. The Jurkat Clone E6-1 CL was found to be genetically instable due to the loss of the amelogenin gene.Conclusions: it was demonstrated by the examples of U937, WISH, WIL2-S, NK-92, and Jurkat Clone E6-1 CLs that STR and the COrDIS plus kit could be used for authentication and genetic stability testing. The obtained results suggest the feasibility of using the COrDIS plus kit for the analysis of CLs used in BCPs, for BCP quality control, and biomedical research.

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