A silver lining in cell line authentication: Short tandem repeat analysis of 1373 cases in China from 2010 to 2019

2021 ◽  
Author(s):  
Meijia Gu ◽  
Meimei Yang ◽  
Jing He ◽  
Sixuan Xia ◽  
Zhe Zhang ◽  
...  
Keyword(s):  
Cell Line ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Cell Line Authentication ◽  
Repeat Analysis ◽  
Silver Lining ◽  
Short Tandem

scholarly journals The Use of Short Tandem Repeat Analysis for Cell Line Authentication

2019 ◽  
Vol 19 (4) ◽  
pp. 251-260
Author(s):  
M. D. Khorolsky ◽  
I. S. Semenova ◽  
E. V. Melnikova ◽  
Yu. V. Olefir
Keyword(s):  
Quality Control ◽  
Cell Line ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Genetic Stability ◽  
Routine Practice ◽  
Stability Testing ◽  
Cell Line Authentication ◽  
Repeat Analysis ◽  
Short Tandem

Short tandem repeat analysis (STR) is a well-established international method of authentication and genetic stability testing of cell lines (CLs). Therefore, the development and introduction of this method into routine practice of cell banks and cell culture collections is a pressing concern. In addition, the expansion of the field of cell-line based biomedical cell products (BСPs) necessitates the implementation of STR as a tool of identification testing during quality control. The State Pharmacopoeia of the Russian Federation does not require mandatory use of STR for cell line identification, while other countries have been using this method for cell line quality control for about a decade. The use of identified CLs in medical practice will ensure the efficacy and safety of BCPs.The aim of the study was to assess the possibility of using STR analysis for authentication and genetic stability testing of CLs using U937, WISH, WIL2-S, NK-92, and Jurkat Clone E6-1 CLs as examples.Materials and me­thods: the following human CLs were used in the study: U937 (ECACC), WISH (ATCC), WIL2S (ATCC), NK-92 (ATCC), and Jurkat Clone E6-1 (ATCC). The CL allelic profiles were determined by STR using the COrDIS Plus kit (Gordiz, Russia). The electrophoretic separation was performed using a Genetic Analyzer 3500 Series instrument. The data provided on the websites of the European Collection of Authenticated Cell Cultures and American Type Culture Collection were used to compare the CL profiles.Results: the AuthentiFiler PCR Amplification Kit (Thermo Fisher Scientific, USA) and the GenePrint 10 System (Promega Corporation, USA) intended for CL authentication by STR were compared with the characteristics of the COrDIS plus kit (Gordiz, Russia). The results of the comparison demonstrated that the COrDIS plus kit includes all the loci found in the foreign kits, as well as the loci recommended by the International Cell Line Authentication Committee. The U-937, WIL2S, and NK-92 CLs demonstrated genetic identity with the reference profiles available on the websites of the international collections. The Jurkat Clone E6-1 CL was found to be genetically instable due to the loss of the amelogenin gene.Conclusions: it was demonstrated by the examples of U937, WISH, WIL2-S, NK-92, and Jurkat Clone E6-1 CLs that STR and the COrDIS plus kit could be used for authentication and genetic stability testing. The obtained results suggest the feasibility of using the COrDIS plus kit for the analysis of CLs used in BCPs, for BCP quality control, and biomedical research.

Determination of sibship by PCR-amplified short tandem repeat analysis in Taiwan

Transfusion ◽  
2000 ◽  
Vol 40 (7) ◽  
pp. 840-845 ◽  
Author(s):  
C.H. Tzeng ◽  
J.Y. Lyou ◽  
Y.R. Chen ◽  
H.Y. Hu ◽  
J.S. Lin ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Repeat Analysis ◽  
Short Tandem

Short Tandem Repeat Analysis on Microfabricated Electrophoretic Devices

2002 ◽  
Author(s):  
Dieter Schmalzing ◽  
Aram Adourian ◽  
Lance Koutny ◽  
Paul Matsudaira ◽  
Daniel Ehrlich
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Repeat Analysis ◽  
Short Tandem

Contribution of ABO-Rhesus/Electrophoresis of hemoglobin methods and Short Tandem Repeats analysis in the determination of paternity in Burkina Faso, West Africa

2020 ◽  
Author(s):  
Missa Millogo ◽  
Serge Theophile Soubeiga ◽  
Bapio Valerie Jean Telesphore Bazie ◽  
Theodora Mahoukede Zohoncon ◽  
Albert Theophane Yonli ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
High Performance ◽  
Short Tandem Repeats ◽  
Tandem Repeats ◽  
Repeat Analysis ◽  
Paternity Index ◽  
Genetic Analyzer ◽  
Short Tandem

Abstract Background: the establishment of filiation by the current ABO, HLA, MNS, Kells and serum tests, pose a real reliability problem. It is then necessary to combine these methods with or to use high-performance methods such as microsatellite genetic analysis or short tandem repeats. This study aimed to compare the short tandem repeat technique with ABO/Rhesus system in combination with electrophoresis of hemoglobin. Methods: Fourteen (14) contested paternity trios were investigated. Blood samples were collected to determine blood groups using the Beth-Vincent method and the type of hemoglobin by electrophoresis. Blood spots on FTA paper were used for the analysis of 16 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA, Amel) by capillary electrophoresis on the ABI 31310 Genetic Analyzer. The generated sequences were analyzed with GeneMapper® software version 3.2.1. The data were analyzed to determine the paternity index and the probability of paternity. Results: Of the fourteen (14) trios studied, ten (10) cases were probable inclusion, three (03) cases were exclusion and one (01) case was an undetermined paternity outcome with the ABO-Rhesus/ electrophoresis of hemoglobin system. While the analysis of genetic polymorphisms in DNA gave five (05) inclusions versus nine (09) exclusions of paternity. Of the 10 probable inclusion cases given by the ABO-Rhesus/Electrophoresis of hemoglobin system, only 05 cases (50%) were confirmed for inclusion by Short tandem repeat analysis. Conclusion: The analysis of short tandem repeat with sixteen genetic markers is more reliable in determining paternity than ABO-Rhesus/hemoglobin electrophoresis techniques.

DNA Profiling via Short Tandem Repeat Analysis by Using Serum Samples

2013 ◽  
Vol 37 (4) ◽  
pp. 220 ◽  
Author(s):  
Ji Hyun Lee ◽  
Hye Young Lee ◽  
Sohee Cho ◽  
Joo Youn Cho ◽  
In Jin Jang ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Dna Profiling ◽  
Serum Samples ◽  
Repeat Analysis ◽  
Short Tandem

Improvement of short tandem repeat analysis of samples highly contaminated by humic acid

2013 ◽  
Vol 20 (7) ◽  
pp. 922-928 ◽  
Author(s):  
Seung Bum Seo ◽  
Hong Xuan Jin ◽  
Hye Young Lee ◽  
Jianye Ge ◽  
Jonathan L. King ◽  
...  
Keyword(s):  
Humic Acid ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Repeat Analysis ◽  
Short Tandem

scholarly journals Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Lieselot Deleye ◽  
Ann-Sophie Vander Plaetsen ◽  
Jana Weymaere ◽  
Dieter Deforce ◽  
Filip Van Nieuwerburgh
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Whole Genome Amplification ◽  
Whole Genome ◽  
Lymphoblastoid Cells ◽  
Genome Amplification ◽  
Repeat Analysis ◽  
Short Tandem

scholarly journals Reuse of Denaturing Polyacrylamide Gels for Short Tandem Repeat Analysis

BioTechniques ◽  
1998 ◽  
Vol 25 (5) ◽  
pp. 892-897 ◽  
Author(s):  
Allan Tereba ◽  
Katherine A. Micka ◽  
James W. Schumm
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Polyacrylamide Gels ◽  
Repeat Analysis ◽  
Short Tandem
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