Mapping the Future of Cell Culture and Cell Line Development

2021 ◽  
Vol 41 (S3) ◽  
pp. S6-S9
Author(s):  
Priya Kalia
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Cell Line Development ◽  
The Future

scholarly journals Rich production media as a platform for CHO cell line development

2020 ◽  
Author(s):  
Yong Jae Kim ◽  
Sang Kyul Han ◽  
Seongtae Yoon ◽  
Chan-Wha Kim
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cells ◽  
Culture Media ◽  
Chinese Hamster ◽  
Cell Cloning ◽  
Cell Line Development ◽  
Solid Media ◽  
Semi Solid ◽  
Set Up

Abstract Recent cell culture media for mammalian cells can be abundantly formulated with nutrients supporting production, but such media can be limited to use in host cell culture, transfection, cell cloning, and cell growth under the low cell density conditions. In many cases, appropriate platform media are used for cell line development, and then replaced with rich media for production. In this study, we demonstrate rich chemically defined media for Chinese hamster ovary (CHO) cells that are suitable as basal media both for cell line development and for final production of culture process. Set up for transfection, semi-solid media optimization, mini-pool screening, and single cell cloning media development were performed, and final clones were obtained with higher productivity in fed-batch culture mode using rich formulated media comparing with lean formulated media. Developed methods may remove the requirements for cell adaptation to production media after cell line development, and relieve the clonality issues associated with changing the culture media. Furthermore, established methods have advantages over traditional approaches, including saving resources and decreasing the time and the effort required to optimize the production process.

scholarly journals A New Integrated Knowledge Management & Data Analysis Platform for Cell Line Development & Cell Culture R&D

2015 ◽  
Vol 9 (S9) ◽  
Author(s):  
Jens Niklas ◽  
Julia Retey ◽  
Steffen Fehrmann ◽  
Ludwig Macko ◽  
Thomas Hartsch ◽  
...  
Keyword(s):  
Cell Culture ◽  
Knowledge Management ◽  
Data Analysis ◽  
Cell Line ◽  
Cell Line Development ◽  
Analysis Platform ◽  
Integrated Knowledge

Intact Protein Mass Spectrometry of Cell Culture Harvest Guides Cell Line Development for Trispecific Antibodies

2020 ◽  
Vol 92 (3) ◽  
pp. 2764-2769
Author(s):  
Fateme Tousi ◽  
Yan Jiang ◽  
Sharmila Sivendran ◽  
Yvonne Song ◽  
Susan Elliott ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Culture ◽  
Cell Line ◽  
Intact Protein ◽  
Cell Line Development ◽  
Protein Mass ◽  
Protein Mass Spectrometry

scholarly journals Tribological, biocompatibility, and antibiofilm properties of tungsten–germanium coating using magnetron sputtering

2021 ◽  
Vol 32 (1) ◽  
Author(s):  
Mustafa Şükrü Kurt ◽  
Mehmet Enes Arslan ◽  
Ayşenur Yazici ◽  
İlkan Mudu ◽  
Elif Arslan
Keyword(s):  
Cell Culture ◽  
Magnetron Sputtering ◽  
Cell Line ◽  
Borosilicate Glass ◽  
Chromosomal Abnormalities ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Antibiofilm Activity ◽  
Borosilicate Glasses

AbstractIn this study, borosilicate glass and 316 L stainless steel were coated with germanium (Ge) and tungsten (W) metals using the Magnetron Sputtering System. Surface structural, mechanical, and tribological properties of uncoated and coated samples were examined using SEM, X-ray diffraction (XRD), energy-dispersive spectroscopy, and tribometer. The XRD results showed that WGe2 chemical compound observed in (110) crystalline phase and exhibited a dense structure. According to the tribological analyses, the adhesion strength of the coated deposition on 316 L was obtained 32.8 N, and the mean coefficient of friction was around 0.3. Biocompatibility studies of coated metallic biomaterials were analyzed on fibroblast cell culture (Primary Dermal Fibroblast; Normal, Human, Adult (HDFa)) in vitro. Hoescht 33258 fluorescent staining was performed to investigate the cellular density and chromosomal abnormalities of the HDFa cell line on the borosilicate glasses coated with germanium–tungsten (W–Ge). Cell viabilities of HDFa cell line on each surface (W–Ge coated borosilicate glass, uncoated borosilicate glass, and cell culture plate surface) were analyzed by using (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cytotoxicity assay. The antibiofilm activity of W–Ge coated borosilicate glass showed a significant reduction effect on Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 27853) adherence compared to control groups. In the light of findings, tungsten and germanium, which are some of the most common industrial materials, were investigated as biocompatible and antimicrobial surface coatings and recommended as bio-implant materials for the first time.

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Arthur H. Intosh ◽  
K. Maramorosch ◽  
C. Rechtoris
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cell ◽  
Insect Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Insect Cell Line ◽  
Cell Culture Medium
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