Simpler Yet Sensitive Glucose Uptake Assay

2016 ◽  
Vol 36 (20) ◽  
pp. 16-17
Author(s):  
Michael Valley ◽  
Jolanta Vidugiriene
Keyword(s):  
Glucose Uptake ◽  
Uptake Assay ◽  
Glucose Uptake Assay

scholarly journals The Cytotoxic Properties of Baeckea frutescens Branches Extracts in Eliminating Breast Cancer Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
S. H. Shahruzaman ◽  
M. F. Mustafa ◽  
S. Ramli ◽  
S. Maniam ◽  
S. Fakurazi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Glucose Uptake ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Glucose Consumption ◽  
Uptake Assay ◽  
Glucose Uptake Assay ◽  
Baeckea Frutescens ◽  
Mcf 7

Breast cancer is the leading cause of cancer death in women in over 100 countries worldwide and accounts for almost 1 in 4 cancer cases among women. Baeckea frutescens of the family Myrtaceae has been used in traditional medicine and is known to possess antibacterial, antipyretic, and cytoprotective properties. In this study, we investigated the role of Baeckea frutescens branches extracts against human breast cancer cells. Baeckea frutescens branches extracts were prepared using Soxhlet apparatus with solvents of different polarity. The selective cytotoxic activity and the glucose consumption rate of Baeckea frutescens branches extracts of various concentrations (20 to 160 ug/ml) at 24-, 48-, and 72-hour time points were studied using MTT and glucose uptake assay. The IC50 values in human breast cancer (MCF-7 and MDA-MB-231) and mammary breast (MCF10A) cell lines were determined. Apoptotic study using AO/PI double staining was performed using fluorescent microscopy. The glucose uptake was measured using 2-NBDG, a fluorescent glucose analogue. The phytochemical screening of major secondary metabolites in plants was performed. This study reports that Baeckea frutescens branches extracts showed potent selective cytotoxic activity against MCF-7 cells compared to MDA-MB-231 cells after 72 hours of treatment. Evidence of early apoptosis which includes membrane blebbing and chromatin condensation was observed after 72 hours of treatment with Baeckea frutescens branches extracts. Interestingly, for the glucose uptake assay, the inhibition was observed as early as 24 hours upon treatment. All Baeckea frutescens extracts showed the presence of major secondary metabolites such as tannin, triterpenoid, flavonoid, and phenol. However, alkaloid level was unable to be determined. The identification of Baeckea frutescens and its possible role in selectively inhibiting glucose consumption in breast cancer cells defines a new role of natural product that can be utilised as an effective agent that regulates metabolic reprogramming in breast cancer.

Development of a cell-based nonradioactive glucose uptake assay system for SGLT1 and SGLT2

2012 ◽  
Vol 429 (1) ◽  
pp. 70-75 ◽  
Author(s):  
Abhinav Kanwal ◽  
Shailendra Pratap Singh ◽  
Paramjit Grover ◽  
Sanjay Kumar Banerjee
Keyword(s):  
Glucose Uptake ◽  
Assay System ◽  
Uptake Assay ◽  
A Cell ◽  
Glucose Uptake Assay

scholarly journals Antidiabetes and Anti-Obesity Activity ofLagerstroemia speciosa

2007 ◽  
Vol 4 (4) ◽  
pp. 401-407 ◽  
Author(s):  
Guy Klein ◽  
Jaekyung Kim ◽  
Klaus Himmeldirk ◽  
Yanyan Cao ◽  
Xiaozhuo Chen
Keyword(s):  
Glucose Uptake ◽  
Glucose Transport ◽  
Cell Model ◽  
Water Soluble ◽  
Published Data ◽  
Functional Screening ◽  
Corosolic Acid ◽  
Uptake Assay ◽  
A Cell ◽  
Glucose Uptake Assay

The leaves ofLagerstroemia speciosa(Lythraceae), a Southeast Asian tree more commonly known as banaba, have been traditionally consumed in various forms by Philippinos for treatment of diabetes and kidney related diseases. In the 1990s, the popularity of this herbal medicine began to attract the attention of scientists worldwide. Since then, researchers have conducted numerousin vitroandin vivostudies that consistently confirmed the antidiabetic activity of banaba. Scientists have identified different components of banaba to be responsible for its activity. Using tumor cells as a cell model, corosolic acid was isolated from the methanol extract of banaba and shown to be an active compound. More recently, a different cell model and the focus on the water soluble fraction of the extract led to the discovery of other compounds. The ellagitannin Lagerstroemin was identified as an effective component of the banaba extract responsible for the activity. In a different approach, using 3T3-L1 adipocytes as a cell model and a glucose uptake assay as the functional screening method, Chenet al. showed that the banaba water extract exhibited an insulin-like glucose transport inducing activity. Coupling HPLC fractionation with a glucose uptake assay, gallotannins were identified in the banaba extract as components responsible for the activity, not corosolic acid. Penta-O-galloyl-glucopyranose (PGG) was identified as the most potent gallotannin. A comparison of published data with results obtained for PGG indicates that PGG has a significantly higher glucose transport stimulatory activity than Lagerstroemin. Chenet al. have also shown that PGG exhibits anti-adipogenic properties in addition to stimulating the glucose uptake in adipocytes. The combination of glucose uptake and anti-adipogenesis activity is not found in the current insulin mimetic drugs and may indicate a great therapeutic potential of PGG.

scholarly journals STUDIES ON IN VITRO ANTIDIABETIC ACTIVITIES OF HOPEA PONGA AND VITEX LEUCOXYLON

2017 ◽  
Vol 9 (2) ◽  
pp. 263 ◽  
Author(s):  
Arun Kashivishwanath Shettar ◽  
Ankala Basappa Vedamurthy
Keyword(s):  
Glucose Uptake ◽  
Aqueous Extract ◽  
Yeast Cells ◽  
Antidiabetic Activity ◽  
Significant Activity ◽  
Soxhlet Apparatus ◽  
Ic50 Value ◽  
Uptake Assay ◽  
Glucose Uptake Assay

<p><strong>Objective: </strong>Evaluating antidiabetic property of <em>Hopea ponga</em> and <em>Vitex leucoxylon</em> extracts by using <em>in vitro</em> assays.</p><p><strong>Methods: </strong>The exhaustive serial extraction was carried out with a series of solvents: chloroform, ethyl acetate, methanol, ethanol and water with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were evaluated for antidiabetic activity by employing standard <em>in vitro</em> techniques (α-amylase and glucose uptake assay using yeast model in which the effects of extracts on α-amylase and glucose uptake was tested by considering the percentage of inhibition of α-amylase and increase in glucose uptake in yeast cells).</p><p><strong>Results: </strong><em>In vitro</em> antidiabetic studies show that in case of <em>Hopea ponga</em> methanol extract showed comparable antidiabetic activity with percentage of α-amylase inhibition 51.7925±0.92794 % and with IC50 value 96.53 µg and it was less on comparison with standard i.e. 71.0907±0.67796% with IC50 value 70.33 µg and in case of glucose uptake assay aqueous extract showed higher activity over all remaining extracts with percentage of inhibition 49.8100±0.62476% and with IC50 value 250.95 µg. whereas in case of <em>Vitex leucoxylon</em> aqueous extract exhibited significant activity in both performed assays i. e α-amylase inhibition and glucose uptake assay with percentage 54.6147±0.46397% and 57.1337±0.44201% respectively when compared to other solvent extracts.</p><p><strong>Conclusion: </strong>Results confirm that aqueous extract of <em>Vitex leucoxylon</em> exhibited highest antidiabetic activity among all extracts. Additional studies are needed for purification, characterization and structural elucidation of bioactive compounds from aqueous extract and also confirm its antidiabetic property by <em>in vivo</em> studies. The present study provides scientific evidence that the leaves of <em>Hopea ponga and Vitex leucoxylon</em> possess anti-diabetic efficacy. Thus, considering its relative antidiabetic potency, these extracts are the useful therapeutic agents for treating and management of diabetes.</p>

Glut1 deficiency syndrome and erythrocyte glucose uptake assay

2011 ◽  
Vol 70 (6) ◽  
pp. 996-1005 ◽  
Author(s):  
Hong Yang ◽  
Dong Wang ◽  
Kristin Engelstad ◽  
Leslie Bagay ◽  
Ying Wei ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Deficiency Syndrome ◽  
Glut1 Deficiency ◽  
Glut1 Deficiency Syndrome ◽  
Uptake Assay ◽  
Glucose Uptake Assay

scholarly journals Erythrocyte 3-O-methyl-D-glucose uptake assay for diagnosis of glucose-transporter-protein syndrome

1999 ◽  
Vol 13 (3) ◽  
pp. 116-121 ◽  
Author(s):  
J�rg Klepper ◽  
Marcela Garcia-Alvarez ◽  
Kevin R. O'Driscoll ◽  
Michael K. Parides ◽  
Dong Wang ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Glucose Transporter ◽  
Transporter Protein ◽  
Uptake Assay ◽  
Glucose Uptake Assay

scholarly journals Synthesis, Characterization, and Preclinical Evaluation of New Thiazolidin-4-ones Substituted with p-Chlorophenoxy Acetic Acid and Clofibric Acid against Insulin Resistance and Metabolic Disorder

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Vasantharaju S. Gowdra ◽  
Jayesh Mudgal ◽  
Punit Bansal ◽  
Pawan G. Nayak ◽  
Seethappa A. Manohara Reddy ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Glucose Uptake ◽  
Metabolic Disorders ◽  
Insulin Resistant ◽  
Insulin Sensitizers ◽  
Uptake Assay ◽  
Glucose Uptake Assay ◽  
Plasma Leptin

We synthesized twenty thiazolidin-4-one derivatives, which were then characterized by standard chromatographic and spectroscopic methods. From thein vitroglucose uptake assay, two compounds behaved as insulin sensitizers, where they enhanced glucose uptake in isolated rat diaphragm. In high-carbohydrate diet-induced insulin resistant mice, these two thiazolidin-4-ones attenuated hyperglycemia, hyperinsulinemia, hypertriglyceridemia, hypercholesterolemia, and glucose intolerance. They raised the plasma leptin but did not reverse the diabetes-induced hypoadiponectinemia. Additionally, compound3areduced adiposity. The test compounds were also able to reverse the disturbed liver antioxidant milieu. To conclude, these two novel thiazolidin-4-ones modulated multiple mechanisms involved in metabolic disorders, reversing insulin resistance and thus preventing the development of type-2 diabetes.

scholarly journals IN VITRO EVALUATION OF ANTIDIABETIC AND CYTOTOXICITY POTENTIALS OF THE RHIZOME EXTRACT OF DRYNARIA QUERCIFOLIA (L.) J. SMITH

2019 ◽  
pp. 72-76
Author(s):  
PRASANNA G ◽  
DEVI R ◽  
ISHWARYA G
Keyword(s):  
Liver Cancer ◽  
Glucose Uptake ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Plant Extract ◽  
Mtt Assay ◽  
Hepg2 Cell Line ◽  
Uptake Assay ◽  
Glucose Uptake Assay

Objective: In the present study, an attempt has been made to evaluate the in vitro antidiabetic and cytotoxic potentials of the rhizome extract of Drynaria quercifolia (L.) J. Smith. Methods: In vitro antidiabetic activity was determined by two different assays such as alpha-amylase inhibition assay and glucose uptake assay. The plant extract with three different concentrations was used for this assay. L6 rat myogenic cells were selected and subjected to glucose uptake assay. The cytotoxic activity of the different concentrations of the plant extract on HepG2 cell line was also investigated in vitro through 3-(4,5, dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results: The findings of the study provide evidence that the rhizome extract of D. quercifolia possesses significant anti-diabetic activity. In MTT assay, the significant cytotoxic effect of plant extract was observed by measuring the percentage of cell viability on the HepG2 cell line. Conclusion: The findings indicated that rhizome extracts of D. quercifolia have potential as a medicinal drug against diabetes mellitus (DM) and liver cancer. Further, studies with in vivo and clinical trials need to be conducted to establish rhizome extract as a safe agent for DM and liver cancer therapy.

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