MicroRNA Profile Differentiates Head and Neck Keloid and Adjacent Normal Skin Tissue

2021 ◽  
Author(s):  
Lamont R. Jones ◽  
Albert M. Levin ◽  
Xiangguo Dai ◽  
Indrani Datta ◽  
Jia Li ◽  
...  
Keyword(s):  
Head And Neck ◽  
Normal Skin ◽  
Skin Tissue ◽  
Microrna Profile

scholarly journals Basophil Recruitment to Skin Lesions of Patients with Systemic Lupus Erythematosus Mediated by CCR1 and CCR2

2017 ◽  
Vol 43 (2) ◽  
pp. 832-839 ◽  
Author(s):  
Qingjun Pan ◽  
Yongmin Feng ◽  
Yanxia Peng ◽  
Hongjiu Zhou ◽  
Zhenzhen Deng ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Migration Rate ◽  
Skin Diseases ◽  
Normal Skin ◽  
Flow Cytometric Analysis ◽  
Skin Lesions ◽  
Skin Tissue ◽  
Systemic Lupus

Background/Aims: Basophils have been reported to infiltrate skin lesions in various skin diseases, but not in systemic lupus erythematosus (SLE). This study investigated basophil infiltration in SLE and its mechanism. Methods: Twenty newly diagnosed SLE patients and twenty healthy controls were enrolled. Nine SLE patients underwent skin biopsies. Flow cytometric analysis the phenotype of peripheral basophils and their migration rate toward RANTES and MCP-1 were analyzed with the transwell culture system, also the expression of these two chemokines in skin tissue were analyzed with immunohistochemistry. Results: Increased activation and decreased numbers of peripheral basophils were observed in SLE patients compared with controls. Basophil migration into skin lesions of SLE patients were observed, but not in normal skin tissue. This migration was related to the upregulation of chemokine receptors CCR1 and CCR2 on basophils. In vitro studies showed that migration rate toward RANTES and MCP-1 increased significantly in basophils from SLE patients compared with those from controls. Consistently, high levels of RANTES and MCP-1 expression were observed in skin lesions from SLE patients but not in normal skin tissue. Conclusion: Basophil recruitment to skin lesions of SLE patients mediated by CCR1 and CCR2, which may contribute to tissue damage in SLE.

MicroRNA Expression Profiling of Cutaneous Squamous Cell Carcinomas Arising in Different Sites

2020 ◽  
Vol 163 (3) ◽  
pp. 538-545
Author(s):  
Alexander N. Rock ◽  
Mason D. Fisher ◽  
Gwenyth Amborski ◽  
Dawn C. Allain ◽  
Victoria Klee ◽  
...  
Keyword(s):  
Head And Neck ◽  
Squamous Cell ◽  
Mirna Expression ◽  
Statistical Significance ◽  
Care Center ◽  
Normal Skin ◽  
Tertiary Care ◽  
Tissue Samples ◽  
Qrt Pcr ◽  
Candidate Mirnas

Objective To examine the microRNA (miRNA) expression profile of cutaneous squamous cell carcinoma (cSCC) tumors from aggressive head and neck locations compared with nonaggressive anatomic sites and normal controls. Study Design Retrospective analysis of miRNA expression. Setting Tertiary care center. Subjects and Methods Tissue samples were collected from 3 anatomic regions: aggressive head and neck sites (ie, ears/lip), nonaggressive locations (ie, extremities/trunk), and adjacent normal skin. RNA was isolated from tissue cores of 45 samples (18 aggressive sites, 15 nonaggressive sites, and 12 normal-adjacent skin). miRNA expression analysis was completed for approximately 800 miRNAs using the NanoString nCounter panel. Five candidate miRNAs were selected for validation. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed on the original samples plus 30 additional tissue samples (7 aggressive sites, 14 nonaggressive sites, and 9 normal-adjacent skin). Results Five candidate miRNAs with significant differences in miRNA expression ( P < 0 ≤ .001) from discovery samples were selected: miR-21, miR-31, let-7g, miR-93, and miR-22. Relative expression for these miRNAs using qRT-PCR in the new sample set did not reveal any significant differences using 1-way analysis of variance. When sets were combined, miR-21 showed increased expression in aggressive tumors relative to nonaggressive tumors ( P = .009), but no others reached statistical significance. Conclusion cSCC behaves more aggressively when originating from specific anatomical subsites of the head and neck. Of 5 miRNAs evaluated, only miR-21 showed significantly higher expression in tumors from aggressive sites relative to nonaggressive sites. Larger sample sizes are needed to evaluate other miRNAs.

scholarly journals LncRNA expression profiles and validation in keloid and normal skin tissue

2015 ◽  
Vol 47 (5) ◽  
pp. 1829-1838 ◽  
Author(s):  
XUEBING LIANG ◽  
LIN MA ◽  
XIAO LONG ◽  
XIAOJUN WANG
Keyword(s):  
Expression Profiles ◽  
Normal Skin ◽  
Skin Tissue ◽  
Lncrna Expression

scholarly journals LEPROMIN-LIKE ACTIVITY OF NORMAL SKIN TISSUE

1958 ◽  
Vol 29 (4) ◽  
Author(s):  
T. F. DAVEY ◽  
S. E. DREWETT
Keyword(s):  
Normal Skin ◽  
Skin Tissue

miRNA Expression Profiles in Keloid Tissue and Corresponding Normal Skin Tissue

2011 ◽  
Vol 36 (1) ◽  
pp. 193-201 ◽  
Author(s):  
Ying Liu ◽  
Daping Yang ◽  
Zhibo Xiao ◽  
Miaobo Zhang
Keyword(s):  
Mirna Expression ◽  
Expression Profiles ◽  
Normal Skin ◽  
Skin Tissue ◽  
Mirna Expression Profiles

Determination of photosensitizer concentration in normal skin tissue and in skin tumor with the use of two-wavelength laser light

1996 ◽  
Author(s):  
Kirill G. Linkov ◽  
Victor B. Loschenov ◽  
Gennady L. Kiselev ◽  
N. J. Edinak ◽  
Rudolf W. Steiner
Keyword(s):  
Laser Light ◽  
Normal Skin ◽  
Skin Tumor ◽  
Skin Tissue

scholarly journals Correlation of CCL8 expression with immune cell infiltration of skin cutaneous melanoma: potential as a prognostic indicator and therapeutic pathway

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Peipei Yang ◽  
Wanrong Chen ◽  
Hua Xu ◽  
Junhan Yang ◽  
Jinghang Jiang ◽  
...  
Keyword(s):  
Cutaneous Melanoma ◽  
Prognostic Model ◽  
Immune Cell ◽  
Cox Regression ◽  
Normal Skin ◽  
Prognostic Indicator ◽  
The Cancer Genome Atlas ◽  
Cancer Prognosis ◽  
Skin Tissue ◽  
M1 Macrophages

Abstract Background The tumor microenvironment (TME) is critical in the progression and metastasis of skin cutaneous melanoma (SKCM). Differences in tumor-infiltrating immune cells (TICs) and their gene expression have been linked to cancer prognosis. Given that immunotherapy can be effective against SKCM, we aimed to identify key genes that regulate the immunological state of the TME in SKCM. Methods Data from 471 SKCM patients in the The Cancer Genome Atlas were analyzed using ESTIMATE algorithms to generate an ImmuneScore, StromalScore, and EstimateScore for each patient. Patients were classified into low- or high-score groups based on median values, then compared in order to identify differentially expressed genes (DEGs). Then a protein–protein interaction (PPI) network was developed, and a prognostic model was created using uni- and multivariate Cox regression as well as the least absolute shrinkage and selection operator (LASSO). Key DEGs were identified using the web-based tool GEPIA. Profiles of TIC subpopulations in each patient were analyzed using CIBORSORT, and possible correlations between key DEG expression and TICs were explored. Levels of CCL8 were determined in SKCM and normal skin tissue using immunohistochemistry. Results Two scores correlated positively with the prognosis of SKCM patients. Comparison of the low- and high-score groups revealed 1684 up-regulated and 18 down-regulated DEGs, all of which were enriched in immune-related functions. The prognostic model identified CCL8 as a key gene, which CIBERSORT found to correlate with M1 macrophages. Immunohistochemistry revealed strong expression in SKCM tissue, but failed to detect the protein in normal skin tissue. Conclusions CCL8 is a potential prognostic marker for SKCM, and it may become an effective target for melanoma in which M1 macrophages play an important role.

scholarly journals Skin tissue engineering advances in severe burns: review and therapeutic applications

2016 ◽  
Vol 4 ◽  
pp. 1-14 ◽  
Author(s):  
Alvin Wen Choong Chua ◽  
Yik Cheong Khoo ◽  
Bien Keem Tan ◽  
Kok Chai Tan ◽  
Chee Liam Foo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Normal Skin ◽  
Donor Site ◽  
Skin Tissue ◽  
Skin Substitutes ◽  
Burn Treatment ◽  
Skin Tissue Engineering ◽  
Severe Burns ◽  
Burn Units

Abstract Current advances in basic stem cell research and tissue engineering augur well for the development of improved cultured skin tissue substitutes: a class of products that is still fraught with limitations for clinical use. Although the ability to grow autologous keratinocytes in-vitro from a small skin biopsy into sheets of stratified epithelium (within 3 to 4 weeks) helped alleviate the problem of insufficient donor site for extensive burn, many burn units still have to grapple with insufficient skin allografts which are used as intermediate wound coverage after burn excision. Alternatives offered by tissue-engineered skin dermal replacements to meet emergency demand have been used fairly successfully. Despite the availability of these commercial products, they all suffer from the same problems of extremely high cost, sub-normal skin microstructure and inconsistent engraftment, especially in full thickness burns. Clinical practice for severe burn treatment has since evolved to incorporate these tissue-engineered skin substitutes, usually as an adjunct to speed up epithelization for wound closure and/or to improve quality of life by improving the functional and cosmetic results long-term. This review seeks to bring the reader through the beginnings of skin tissue engineering, the utilization of some of the key products developed for the treatment of severe burns and the hope of harnessing stem cells to improve on current practice.

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