Identification of Differentially Expressed Circular RNAs in Keloid and Normal Skin Tissue by High‐throughput Sequencing

2021 ◽  
Author(s):  
Jing Zhang ◽  
Ninghua Liu ◽  
Xiufa Wu ◽  
Peixuan Wu ◽  
Nan Song ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Normal Skin ◽  
Differentially Expressed ◽  
Skin Tissue ◽  
Circular Rnas

scholarly journals seekCRIT: Detecting and characterizing differentially expressed circular RNAs using high-throughput sequencing data

2020 ◽  
Vol 16 (10) ◽  
pp. e1008338
Author(s):  
Mohamed Chaabane ◽  
Kalina Andreeva ◽  
Jae Yeon Hwang ◽  
Tae Lim Kook ◽  
Juw Won Park ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Differentially Expressed ◽  
Circular Rnas ◽  
Sequencing Data ◽  
High Throughput Sequencing Data

scholarly journals Identification of Exogenous Nitric Oxide-Responsive miRNAs from Alfalfa (Medicago sativa L.) under Drought Stress by High-Throughput Sequencing

Genes ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 30
Author(s):  
Yaodong Zhao ◽  
Wenjing Ma ◽  
Xiaohong Wei ◽  
Yu Long ◽  
Ying Zhao ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Drought Stress ◽  
Medicago Sativa ◽  
High Throughput ◽  
Drought Resistance ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Medicago Sativa L

Alfalfa (Medicago sativa L.) is a high quality leguminous forage. Drought stress is one of the main factors that restrict the development of the alfalfa industry. High-throughput sequencing was used to analyze the microRNA (miRNA) profiles of alfalfa plants treated with CK (normal water), PEG (polyethylene glycol-6000; drought stress), and PEG + SNP (sodium nitroprusside; nitric oxide (NO) sprayed externally under drought stress). We identified 90 known miRNAs belonging to 46 families and predicted 177 new miRNAs. Real-time quantitative fluorescent PCR (qRT-PCR) was used to validate high-throughput expression analysis data. A total of 32 (14 known miRNAs and 18 new miRNAs) and 55 (24 known miRNAs and 31 new miRNAs) differentially expressed miRNAs were identified in PEG and PEG + SNP samples. This suggested that exogenous NO can induce more new miRNAs. The differentially expressed miRNA maturation sequences in the two treatment groups were targeted by 86 and 157 potential target genes, separately. The function of target genes was annotated by gene ontology (GO) enrichment and kyoto encyclopedia of genes and genomes (KEGG) analysis. The expression profiles of nine selected miRNAs and their target genes verified that their expression patterns were opposite. This study has documented that analysis of miRNA under PEG and PEG + SNP conditions provides important insights into the improvement of drought resistance of alfalfa by exogenous NO at the molecular level. This has important scientific value and practical significance for the improvement of plant drought resistance by exogenous NO.

scholarly journals Identification and study of differentially expressed miRNAs in aged NAFLD rats based on high-throughput sequencing

2020 ◽  
Vol 19 (3) ◽  
pp. 302-312 ◽  
Author(s):  
Ying Zhang ◽  
Danni Xiang ◽  
Xiaona Hu ◽  
Qingwei Ruan ◽  
Lina Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Differentially Expressed ◽  
Differentially Expressed Mirnas

scholarly journals Microarray Analysis And Functional Prediction Of Differentially-Expressed Circular RNAs In Acquired Middle Ear Choleateatoma

2021 ◽  
Author(s):  
Shumin Xie ◽  
Li Jin ◽  
Tuanfang Yin ◽  
Jihao Ren ◽  
Wei Liu
Keyword(s):  
Microarray Analysis ◽  
Middle Ear ◽  
Expression Profiling ◽  
Normal Skin ◽  
Differentially Expressed ◽  
Circular Rnas ◽  
Functional Prediction ◽  
High Confidence ◽  
Network Analyses ◽  
Middle Ear Cholesteatoma

Abstract Background: Middle ear cholesteatoma is characterized by hyper-proliferation of keratinocytes. Circular RNA (circRNA) plays an essential role in the pathogenesis of many proliferative diseases. However, the role of circRNA in the etiopathogenesis of middle ear cholesteatoma is rarely investigated so far. Our aim was to investigate the differential expression profiling of circRNAs between acquired middle ear cholesteatoma and normal skin, and to indentify potential circRNAs contributing to the etiopathogenesis of middle ear cholesteatoma. Microarray analysis and functional prediction were performed to investigate the circRNA expression profiling between middle ear cholesteatoma and normal skin. Validation of differentially expressed circRNAs was conducted by qRT-PCR. Prediction of m6A modification was also carried out. Results: Microarray analysis displayed that totally 93 up-regulated and 85 down-regulated circRNAs were identified in middle ear cholesteatoma. Through validation, expressions of hsa_circRNA_104327 and hsa_circRNA_404655 were significantly higher, while hsa_circRNA_000319 was significantly down-regulated in cholesteatoma. GO classification, KEGG pathway, and ceRNA network analyses suggested that these differentially expressed circRNAs might play importantant roles in the etiopathogenesis of middle ear cholesteatoma. Prediction of m6A modification exhibited that hsa_circRNA_000319 possessed 4 m6A sites with very high confidence, and hsa_circRNA_404655 had 3 m6A sites with high confidence.Conclusions: Our study indicated possible roles of differentially expressed circRNAs in the etiopathogenesis of middle ear cholesteatoma. Targeting on these differentially expressed circRNAs may provide a new and effective treatment for middle ear cholesteatoma in the future.

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