Antimicrobial Resistance and Molecular Characterization of Class 1 Integron in Salmonella Isolates Recovered from Pig Farms in Chongqing, China

2021 ◽  
Author(s):  
Chao Ye ◽  
Dongyi Xu ◽  
Jing Chen ◽  
Fengqing Hou ◽  
Yu Wang ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Class 1 Integron ◽  
Pig Farms ◽  
Class 1

scholarly journals Bacteremia caused by an Acinetobacter junii strain harboring class 1 integron and diverse DNA mobile elements

2014 ◽  
Vol 8 (05) ◽  
pp. 666-669 ◽  
Author(s):  
German M Traglia ◽  
Marisa Almuzara ◽  
Elisabet Vilacoba ◽  
Alicia Tuduri ◽  
Gabriela Neumann ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Renal Transplant Patient ◽  
Transplant Patient ◽  
Mobile Elements ◽  
Ocular Infection ◽  
Class 1 Integron ◽  
Acinetobacter Junii ◽  
Genomic Plasticity ◽  
Class 1

Introduction: Infections caused by Acinetobacter junii are rarely reported. However, some outbreaks of septicemia in neonates and pediatric oncology patients, as well as meningitis, peritonitis, and ocular infection have been described. Since it is highly infrequent to find the molecular characterization of A. junii strains in literature, in this study we described the molecular characterization of A. junii isolates recovered from blood samples of a renal transplant patient. Methodology: The case was defined as a catheter-related bacteremia caused by A. junii. The patient responded favorably after catheter removal and treatment with ciprofloxacin. Results and Conclusion: The complete molecular characterization of the isolate showed that it harbored a class 1 integron and diverse DNA mobile elements. This explains its genomic plasticity for acquiring antimicrobial resistance determinants and for adapting to a nosocomial niche.

scholarly journals Molecular Characterization of β-Lactamase Genes and Their Genetic Structures in Acinetobacter Genospecies 3 Isolates in Taiwan

2010 ◽  
Vol 54 (6) ◽  
pp. 2699-2703 ◽  
Author(s):  
Li-Yueh Huang ◽  
Po-Liang Lu ◽  
Te-Li Chen ◽  
Fang-Yee Chang ◽  
Chang-Phone Fung ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Molecular Characterization ◽  
Class 1 Integron ◽  
Integrase Gene ◽  
Class 1 ◽  
Genetic Structures

ABSTRACT The genetic structure of β-lactamases in Acinetobacter genospecies 3 (AG3) isolates in Taiwan was studied to analyze their high rates of resistance to β-lactams, including carbapenems (57.9%). bla IMP-1 and bla IMP-8 were located in a class 1 integron. bla OXA-58 was bracketed by ISAba3. A novel TnpF-like integrase gene was identified upstream of bla VEB-3. Adjacent to the 5′ sequence of the bla ADC gene, folE was identified. Four new Acinetobacter-derived cephalosporinase (ADC) enzymes were found, which clustered phylogenetically with published AG3 ADC proteins.

scholarly journals Molecular Characterization of ISCR1-MediatedblaPER-1in a Non-O1, Non-O139 Vibrio cholerae Strain from China

2015 ◽  
Vol 59 (7) ◽  
pp. 4293-4295 ◽  
Author(s):  
Jun Wu ◽  
Lianyan Xie ◽  
Fangfang Zhang ◽  
Yuxing Ni ◽  
Jingyong Sun
Keyword(s):  
Vibrio Cholerae ◽  
Molecular Characterization ◽  
Cholerae Strain ◽  
Class 1 Integron ◽  
Content Type ◽  
Conserved Sequence ◽  
Circular Molecule ◽  
Class 1 ◽  
Insight Into

ABSTRACTWe report the detection of PER-1 extended-spectrum β-lactamase (ESBL) in a clinical non-O1, non-O139Vibrio choleraestrain from China. ISCR1-mediatedblaPER-1was embedded in a complex In4family class 1 integron belonging to the lineage of Tn1696on a conjugative IncA/C plasmid. A free 8.98-kb circular molecule present with the ISCR1-blaPER-1–truncated 3′-conserved sequence (CS) structure was detected in this isolate. These findings may provide insight into the mobilization ofblaPER-1.

scholarly journals Molecular Characterization of Integrons inAcinetobacter baumannii: Description of a Hybrid Class 2 Integron

2000 ◽  
Vol 44 (10) ◽  
pp. 2684-2688 ◽  
Author(s):  
Marie-Cécile Ploy ◽  
François Denis ◽  
Patrice Courvalin ◽  
Thierry Lambert
Keyword(s):  
Multidrug Resistance ◽  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Hybrid Structure ◽  
Class 1 Integron ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Hybrid Class ◽  
Unknown Gene

ABSTRACT Twenty Acinetobacter baumannii strains resistant to various antibiotics were analyzed for integron content and sequences of the amplification products. Sixteen clinical isolates had a class 1 integron, 2 contained an additional class 1 or class 2 integron, but no class 3 integron was detected. Thirteen strains had integrons with a single cassette: aac(3)-Ia (9 strains), ant(2")-Ia (2 strains), or aac(6′)-Ib (2 strains); 1 hadaac(6′)-Ib and oxa20cassettes and an unknown gene; and 1 had an integron containingant(2")-Ia and an oxa3cassette truncated by IS6100. The remaining strains harbored class 1 integrons with gene cassettes previously found inEnterobacteriaceae. One integron had a hybrid structure composed of intI2 and the 3′ conserved segment of class 1 integrons. These data indicate that integrons play a major role in multidrug resistance in Acinetobacter.

scholarly journals Characterization of an Integron Carrying blaIMP-1 and a New Aminoglycoside Resistance Gene, aac(6′)-31, and Its Dissemination among Genetically Unrelated Clinical Isolates in a Brazilian Hospital

2007 ◽  
Vol 51 (7) ◽  
pp. 2611-2614 ◽  
Author(s):  
Rodrigo E. Mendes ◽  
Mariana Castanheira ◽  
Mark A. Toleman ◽  
Helio S. Sader ◽  
Ronald N. Jones ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Pseudomonas Putida ◽  
Resistance Gene ◽  
Clinical Isolate ◽  
Hospitalized Patients ◽  
Aminoglycoside Resistance ◽  
Class 1 Integron ◽  
Resistance Determinants ◽  
Class 1

ABSTRACT Seven bla IMP-1-harboring Acinetobacter sp. isolates and one Pseudomonas putida clinical isolate were recovered from hospitalized patients. All isolates possessed a class 1 integron, named In86, carrying the same cassette array [bla IMP1, aac(6′)-31, and aadA1], which was plasmid located in five of the isolates. This report describes the ability of nonfermentative nosocomial pathogens to acquire and disseminate antimicrobial resistance determinants.

scholarly journals Evidence for a Chromosomally Located Third Integron in Salmonella enterica Serovar Typhimurium DT104b

2004 ◽  
Vol 48 (4) ◽  
pp. 1350-1352 ◽  
Author(s):  
M. Daly ◽  
J. Buckley ◽  
E. Power ◽  
S. Fanning
Keyword(s):  
Antimicrobial Resistance ◽  
Genetic Mapping ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Bacterial Chromosome ◽  
Class 1 Integron ◽  
Phage Types ◽  
Serovar Typhimurium ◽  
Class 1

ABSTRACT Salmonella enterica serovar Typhimurium isolates of phage types DT104 and DT104b are frequently associated with multiple antimicrobial resistance. We describe the characterization of a class 1 integron containing dfrA1 and aadA1, genes from two Salmonella serovar Typhimurium DT104b isolates. Genetic mapping located the integron to the bacterial chromosome in each case.

scholarly journals Occurrence and molecular characterization of metallo-β-lactamases (MBLs) among Acinetobacter baumannii isolates from cancer patients

10.3823/821 ◽  
2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Husam F. Qouzah ◽  
Feras Hawari ◽  
Luay F. Abu-Qatouseh ◽  
Asem A. Shehabi
Keyword(s):  
Acinetobacter Baumannii ◽  
Cancer Patients ◽  
Molecular Characterization ◽  
Wide Spectrum ◽  
Multidrug Resistant ◽  
Class 1 Integron ◽  
Class B ◽  
Class 1 ◽  
Resistant Infection

Background: During the last decade, the prevalence of carbapenem-resistant infection associated with multidrug resistant (MDR) Acinetobacter baumanniiin patients has been continuously increasing.  This prospective study aimed to determine the occurrence and molecular characterization of metallo-β-lactamases (MBLs) and carbapenem hydrolyzing oxacillinases among A. baumannii isolates from cancer patients over a period of 6-month. Methods:Antimicrobial susceptibility profile of 70 randomly collected A. baumannii isolates was first determined using disc diffusion test, and second, the MICs of 45 representative multidrug resistant (MDR) isolates were tested to useful drugs in treatment of their infections using E-test.  PCR assays were used to detect the common four types of class D carbapenem hydrolyzing oxacillinases, two types of class A carbapenemases, four types of class B metallo-β –lactamases, and prevalence of Class 1 Integron among MDR isolates. Results: All 70 isolates were MDR, including 100% resistance to meropenom, aztreonem, piperacillin/tazobactum and 99% to carbapenem.  All isolates carried blaOXA-23 and blaOXA-51, but none carried a blaOXA-24 like or blaOXA-58. The isolates also were positive for NDM-1, NDM, VIM, GES, KPC and SPM at the rates of 29%, 20%, 29%,19%,7% and 2%, respectively.  Class 1 Integron was positive in 82% of A. baumanniiisolates. The clonal relationship of 42 MDR A. baumanniiisolates using ERIC-PCR and constructed dendrogram showed 3 major genotype clusters of genetically related isolates. These include 4 genotype groups, each composed of 2 isolates with 100 % similarity of DNA bands.  Conclusion:This study demonstrates that A. baumannii colonize frequently cancer patients in association with antibiotic treatment. The organism is mostly carrying wide spectrum of antibiotic resistance genetic factors, especially many types of ESBLs and MBLs and Class 1 Integron. This fact should be considered when therapy is selected for treatment of patients infected with MDR A. baumannii. Key words. Acinetobacter baumannii, ESBLs, MBLs, Class 1 Integron, Jordanian cancer patients.  

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