scholarly journals Occurrence and molecular characterization of metallo-β-lactamases (MBLs) among Acinetobacter baumannii isolates from cancer patients

10.3823/821 ◽  
2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Husam F. Qouzah ◽  
Feras Hawari ◽  
Luay F. Abu-Qatouseh ◽  
Asem A. Shehabi
Keyword(s):  
Acinetobacter Baumannii ◽  
Cancer Patients ◽  
Molecular Characterization ◽  
Wide Spectrum ◽  
Multidrug Resistant ◽  
Class 1 Integron ◽  
Class B ◽  
Class 1 ◽  
Resistant Infection

Background: During the last decade, the prevalence of carbapenem-resistant infection associated with multidrug resistant (MDR) Acinetobacter baumanniiin patients has been continuously increasing.  This prospective study aimed to determine the occurrence and molecular characterization of metallo-β-lactamases (MBLs) and carbapenem hydrolyzing oxacillinases among A. baumannii isolates from cancer patients over a period of 6-month. Methods:Antimicrobial susceptibility profile of 70 randomly collected A. baumannii isolates was first determined using disc diffusion test, and second, the MICs of 45 representative multidrug resistant (MDR) isolates were tested to useful drugs in treatment of their infections using E-test.  PCR assays were used to detect the common four types of class D carbapenem hydrolyzing oxacillinases, two types of class A carbapenemases, four types of class B metallo-β –lactamases, and prevalence of Class 1 Integron among MDR isolates. Results: All 70 isolates were MDR, including 100% resistance to meropenom, aztreonem, piperacillin/tazobactum and 99% to carbapenem.  All isolates carried blaOXA-23 and blaOXA-51, but none carried a blaOXA-24 like or blaOXA-58. The isolates also were positive for NDM-1, NDM, VIM, GES, KPC and SPM at the rates of 29%, 20%, 29%,19%,7% and 2%, respectively.  Class 1 Integron was positive in 82% of A. baumanniiisolates. The clonal relationship of 42 MDR A. baumanniiisolates using ERIC-PCR and constructed dendrogram showed 3 major genotype clusters of genetically related isolates. These include 4 genotype groups, each composed of 2 isolates with 100 % similarity of DNA bands.  Conclusion:This study demonstrates that A. baumannii colonize frequently cancer patients in association with antibiotic treatment. The organism is mostly carrying wide spectrum of antibiotic resistance genetic factors, especially many types of ESBLs and MBLs and Class 1 Integron. This fact should be considered when therapy is selected for treatment of patients infected with MDR A. baumannii. Key words. Acinetobacter baumannii, ESBLs, MBLs, Class 1 Integron, Jordanian cancer patients.  

scholarly journals Molecular Characterization of Integrons inAcinetobacter baumannii: Description of a Hybrid Class 2 Integron

2000 ◽  
Vol 44 (10) ◽  
pp. 2684-2688 ◽  
Author(s):  
Marie-Cécile Ploy ◽  
François Denis ◽  
Patrice Courvalin ◽  
Thierry Lambert
Keyword(s):  
Multidrug Resistance ◽  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Hybrid Structure ◽  
Class 1 Integron ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Hybrid Class ◽  
Unknown Gene

ABSTRACT Twenty Acinetobacter baumannii strains resistant to various antibiotics were analyzed for integron content and sequences of the amplification products. Sixteen clinical isolates had a class 1 integron, 2 contained an additional class 1 or class 2 integron, but no class 3 integron was detected. Thirteen strains had integrons with a single cassette: aac(3)-Ia (9 strains), ant(2")-Ia (2 strains), or aac(6′)-Ib (2 strains); 1 hadaac(6′)-Ib and oxa20cassettes and an unknown gene; and 1 had an integron containingant(2")-Ia and an oxa3cassette truncated by IS6100. The remaining strains harbored class 1 integrons with gene cassettes previously found inEnterobacteriaceae. One integron had a hybrid structure composed of intI2 and the 3′ conserved segment of class 1 integrons. These data indicate that integrons play a major role in multidrug resistance in Acinetobacter.

scholarly journals Characterization of the New Metallo-β-Lactamase VIM-13 and Its Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in Spain

2008 ◽  
Vol 52 (10) ◽  
pp. 3589-3596 ◽  
Author(s):  
Carlos Juan ◽  
Alejandro Beceiro ◽  
Olivia Gutiérrez ◽  
Sebastián Albertí ◽  
Margalida Garau ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pcr Amplification ◽  
Class 1 Integron ◽  
Negative Results ◽  
New Variant ◽  
Class B ◽  
Class 1 ◽  
Encoding Gene ◽  
Good Agreement

ABSTRACT During a survey conducted to evaluate the incidence of class B carbapenemase (metallo-β-lactamase [MBL])-producing Pseudomonas aeruginosa strains from hospitals in Majorca, Spain, five clinical isolates showed a positive Etest MBL screening test result. In one of them, strain PA-SL2, the presence of a new bla VIM derivative (bla VIM-13) was detected by PCR amplification with bla VIM-1-specific primers followed by sequencing. The bla VIM-13-producing isolate showed resistance to all β-lactams (except aztreonam), gentamicin, tobramycin, and ciprofloxacin. VIM-13 exhibited 93% and 88% amino acid sequence identities with VIM-1 and VIM-2, respectively. bla VIM-13 was cloned in parallel with bla VIM-1, and the resistance profile conferred was analyzed both in Escherichia coli and in P. aeruginosa backgrounds. Compared to VIM-1, VIM-13 conferred slightly higher levels of resistance to piperacillin and lower levels of resistance to ceftazidime and cefepime. VIM-13 and VIM-1 were purified in parallel as well, and their kinetic parameters were compared. The k cat/K m ratios for the antibiotics mentioned above were in good agreement with the MIC data. Furthermore, EDTA inhibited the activity of VIM-13 approximately 25 times less than it inhibited the activity of VIM-1. VIM-13 was harbored in a class 1 integron, along with a new variant (Ala108Thr) of the aminoglycoside-modifying enzyme encoding gene aacA4, which confers resistance to gentamicin and tobramycin. Finally, the VIM-13 integron was apparently located in the chromosome, since transformation and conjugation experiments consistently yielded negative results and the bla VIM-13 probe hybridized only with the genomic DNA.

scholarly journals Bacteremia caused by an Acinetobacter junii strain harboring class 1 integron and diverse DNA mobile elements

2014 ◽  
Vol 8 (05) ◽  
pp. 666-669 ◽  
Author(s):  
German M Traglia ◽  
Marisa Almuzara ◽  
Elisabet Vilacoba ◽  
Alicia Tuduri ◽  
Gabriela Neumann ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Renal Transplant Patient ◽  
Transplant Patient ◽  
Mobile Elements ◽  
Ocular Infection ◽  
Class 1 Integron ◽  
Acinetobacter Junii ◽  
Genomic Plasticity ◽  
Class 1

Introduction: Infections caused by Acinetobacter junii are rarely reported. However, some outbreaks of septicemia in neonates and pediatric oncology patients, as well as meningitis, peritonitis, and ocular infection have been described. Since it is highly infrequent to find the molecular characterization of A. junii strains in literature, in this study we described the molecular characterization of A. junii isolates recovered from blood samples of a renal transplant patient. Methodology: The case was defined as a catheter-related bacteremia caused by A. junii. The patient responded favorably after catheter removal and treatment with ciprofloxacin. Results and Conclusion: The complete molecular characterization of the isolate showed that it harbored a class 1 integron and diverse DNA mobile elements. This explains its genomic plasticity for acquiring antimicrobial resistance determinants and for adapting to a nosocomial niche.

scholarly journals Diversity and Evolution of AbaR Genomic Resistance Islands in Acinetobacter baumannii Strains of European Clone I

2011 ◽  
Vol 55 (7) ◽  
pp. 3201-3206 ◽  
Author(s):  
Lenka Krizova ◽  
Lenie Dijkshoorn ◽  
Alexandr Nemec
Keyword(s):  
Acinetobacter Baumannii ◽  
Selective Advantage ◽  
Multidrug Resistant ◽  
Original Form ◽  
Class 1 Integron ◽  
Content Type ◽  
Atpase Gene ◽  
Class 1 ◽  
Major Clone ◽  
Derivatives Of

ABSTRACTTo assess the diversity of AbaR genomic resistance islands inAcinetobacter baumanniiEuropean clone I (MLST clonal complex 1), we investigated 26 multidrug-resistant strains of this major clone isolated from hospitals in 21 cities of 10 European countries between 1984 and 2005. Each strain harbored an AbaR structure integrated at the same position in the chromosomal ATPase gene. AbaR3, including four subtypes based on variations in class 1 integron cassettes, and AbaR10 were found in 15 and 2 strains, respectively, whereas a new, unique AbaR variant was discovered in each of the other 9 strains. These new variants, designated AbaR11 to AbaR19 (19.8 kb to 57.5 kb), seem to be truncated derivatives of AbaR3, likely resulting from the deletions of its internal parts mediated by either IS26elements (AbaR12 to AbaR19) or homologous recombination (AbaR11). AbaR3 was detected in all 10 strains isolated in 1984 to 1991, while AbaR11 to AbaR19 were carried only by strains isolated since 1997. Our results and those from previous publications suggest that AbaR3 is the original form of AbaR in European clone I, which may have provided strains of the lineage with a selective advantage facilitating their spread in European hospitals in the 1980s or before.

scholarly journals Dissemination of multidrug-resistant, class 1 integron-carrying Acinetobacter baumannii isolates in Taiwan

2008 ◽  
Vol 14 (11) ◽  
pp. 1010-1019 ◽  
Author(s):  
L.-Y. Huang ◽  
T.-L. Chen ◽  
P.-L. Lu ◽  
C.-A. Tsai ◽  
W.-L. Cho ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Multidrug Resistant ◽  
Class 1 Integron ◽  
Class 1

scholarly journals Molecular Characterization of β-Lactamase Genes and Their Genetic Structures in Acinetobacter Genospecies 3 Isolates in Taiwan

2010 ◽  
Vol 54 (6) ◽  
pp. 2699-2703 ◽  
Author(s):  
Li-Yueh Huang ◽  
Po-Liang Lu ◽  
Te-Li Chen ◽  
Fang-Yee Chang ◽  
Chang-Phone Fung ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Molecular Characterization ◽  
Class 1 Integron ◽  
Integrase Gene ◽  
Class 1 ◽  
Genetic Structures

ABSTRACT The genetic structure of β-lactamases in Acinetobacter genospecies 3 (AG3) isolates in Taiwan was studied to analyze their high rates of resistance to β-lactams, including carbapenems (57.9%). bla IMP-1 and bla IMP-8 were located in a class 1 integron. bla OXA-58 was bracketed by ISAba3. A novel TnpF-like integrase gene was identified upstream of bla VEB-3. Adjacent to the 5′ sequence of the bla ADC gene, folE was identified. Four new Acinetobacter-derived cephalosporinase (ADC) enzymes were found, which clustered phylogenetically with published AG3 ADC proteins.

scholarly journals Identification and Characterization of Class 1 Integron Resistance Gene Cassettes among Salmonella Strains Isolated from Imported Seafood

2008 ◽  
Vol 75 (4) ◽  
pp. 1192-1196 ◽  
Author(s):  
Ashraf A. Khan ◽  
Elizabeth Ponce ◽  
M. S. Nawaz ◽  
Chorng-Ming Cheng ◽  
Junaid A. Khan ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Southern Hybridization ◽  
Multidrug Resistant ◽  
The Philippines ◽  
Streptomycin Resistance ◽  
Class 1 Integron ◽  
Class 1 ◽  
Identification And Characterization ◽  
Multiple Antibiotics

ABSTRACT A total of 210 Salmonella isolates, representing 64 different serovars, were isolated from imported seafood samples, and 55/210 isolates were found to be resistant to at least one antibiotic. Class 1 integrons from three multidrug-resistant Salmonella enterica strains (Salmonella enterica serovars Newport [strain 62], Typhimurium var. Copenhagen [strain 629], and Lansing [strain 803], originating from Hong Kong, the Philippines, and Taiwan, respectively) were characterized. Southern hybridization of plasmids isolated from these strains, using a class 1 integron probe, showed that trimethoprim-sulfamethoxazole and streptomycin resistance genes were located on a megaplasmid in strain 629. Our study indicates that imported seafood could be a reservoir for Salmonella isolates resistant to multiple antibiotics.

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