scholarly journals Food Microbial Pathogen Detection and Analysis Using DNA Microarray Technologies

2008 ◽  
Vol 5 (4) ◽  
pp. 531-550 ◽  
Author(s):  
Avraham Rasooly ◽  
Keith E. Herold
Keyword(s):  
Dna Microarray ◽  
Pathogen Detection ◽  
Microbial Pathogen

Multifunctional Printable Micropattern Array for Digital Nucleic Acid Assay for Microbial Pathogen Detection

2021 ◽  
Vol 13 (2) ◽  
pp. 3098-3108
Author(s):  
Yunho Choi ◽  
Younseong Song ◽  
Yong Tae Kim ◽  
Seok Jae Lee ◽  
Kyoung G. Lee ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Pathogen Detection ◽  
Microbial Pathogen ◽  
Nucleic Acid Assay

scholarly journals A Resistance Based Biosensor That Utilizes Conductive Microfibers for Microbial Pathogen Detection

2012 ◽  
Vol 01 (03) ◽  
pp. 36-43 ◽  
Author(s):  
Shannon K. McGraw ◽  
Evangelyn Alocilja ◽  
Kris Senecal ◽  
Andre Senecal
Keyword(s):  
Pathogen Detection ◽  
Microbial Pathogen

Comparison of conventional culture with SeptiFast real-time PCR for microbial pathogen detection in clinical specimens other than blood

2011 ◽  
Vol 60 (12) ◽  
pp. 1774-1778 ◽  
Author(s):  
Antonella Mencacci ◽  
Christian Leli ◽  
Angela Cardaccia ◽  
Paolo Montagna ◽  
Amedeo Moretti ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pathogen Detection ◽  
Clinical Specimens ◽  
Microbial Pathogen ◽  
Conventional Culture

scholarly journals Rapid detection methods of microbial pathogens in foods – a short survey

2021 ◽  
Vol 8 (1) ◽  
pp. 5-22
Author(s):  
Barbora Vidová ◽  
Andrej Godány ◽  
Ernest Šturdík
Keyword(s):  
Nucleic Acid ◽  
Rapid Detection ◽  
Pathogen Detection ◽  
Microbial Pathogens ◽  
Detection Methods ◽  
Laboratory Methods ◽  
Microbial Pathogen ◽  
Short Survey ◽  
Wide Range ◽  
The World

During harvesting, processing and handling operations foods may become contaminated with a wide range of microorganisms. This paper is presented as a short survey of recent used laboratory methods for foods microbial pathogen detection, briefly summarizing rapid, specific and sensitive methods useful for foods testing based on immunochemical and nucleic acid technologies. As the world becomes more concerned with safe foods, the demand for rapid detecting will only increase.

scholarly journals Nucleic Acid Amplification Strategies for DNA Microarray-Based Pathogen Detection

2004 ◽  
Vol 70 (5) ◽  
pp. 3047-3054 ◽  
Author(s):  
Gary J. Vora ◽  
Carolyn E. Meador ◽  
David A. Stenger ◽  
Joanne D. Andreadis
Keyword(s):  
Nucleic Acid ◽  
Multiplex Pcr ◽  
Dna Microarray ◽  
Pathogen Detection ◽  
Pcr Amplification ◽  
Environmental Water ◽  
Nucleic Acid Amplification ◽  
Klenow Fragment ◽  
Front End ◽  
Random Amplification

ABSTRACT DNA microarray-based screening and diagnostic technologies have long promised comprehensive testing capabilities. However, the potential of these powerful tools has been limited by front-end target-specific nucleic acid amplification. Despite the sensitivity and specificity associated with PCR amplification, the inherent bias and limited throughput of this approach constrain the principal benefits of downstream microarray-based applications, especially for pathogen detection. To begin addressing alternative approaches, we investigated four front-end amplification strategies: random primed, isothermal Klenow fragment-based, φ29 DNA polymerase-based, and multiplex PCR. The utility of each amplification strategy was assessed by hybridizing amplicons to microarrays consisting of 70-mer oligonucleotide probes specific for enterohemorrhagic Escherichia coli O157:H7 and by quantitating their sensitivities for the detection of O157:H7 in laboratory and environmental samples. Although nearly identical levels of hybridization specificity were achieved for each method, multiplex PCR was at least 3 orders of magnitude more sensitive than any individual random amplification approach. However, the use of Klenow-plus-Klenow and φ29 polymerase-plus-Klenow tandem random amplification strategies provided better sensitivities than multiplex PCR. In addition, amplification biases among the five genetic loci tested were 2- to 20-fold for the random approaches, in contrast to >4 orders of magnitude for multiplex PCR. The same random amplification strategies were also able to detect all five diagnostic targets in a spiked environmental water sample that contained a 63-fold excess of contaminating DNA. The results presented here underscore the feasibility of using random amplification approaches and begin to systematically address the versatility of these approaches for unbiased pathogen detection from environmental sources.

Transmutation of Personal Glucose Meters into Portable and Highly Sensitive Microbial Pathogen Detection Platform

Small ◽  
2015 ◽  
Vol 11 (37) ◽  
pp. 4970-4975 ◽  
Author(s):  
Zhenzhen Wang ◽  
Zhaowei Chen ◽  
Nan Gao ◽  
Jinsong Ren ◽  
Xiaogang Qu
Keyword(s):  
Pathogen Detection ◽  
Microbial Pathogen ◽  
Highly Sensitive

Microbial Pathogen Detection Strategies

2010 ◽  
pp. 1-4
Author(s):  
Diana Vanegas ◽  
Jonathan Claussen ◽  
Eric McLamore ◽  
Carmen Gomes
Keyword(s):  
Pathogen Detection ◽  
Microbial Pathogen ◽  
Detection Strategies

Development of DNA microarray for pathogen detection

2004 ◽  
Vol 9 (2) ◽  
pp. 93-99 ◽  
Author(s):  
Seung Min Yoo ◽  
Ki Chang Keum ◽  
So Young Yoo ◽  
Jun Yong Choi ◽  
Kyung Hee Chang ◽  
...  
Keyword(s):  
Dna Microarray ◽  
Pathogen Detection
Sign in / Sign up

Export Citation Format

Share Document