scholarly journals Individualized Medicine for Renal Cell Carcinoma: Establishment of Primary Cell Line Culture from Surgical Specimens

2008 ◽  
Vol 22 (10) ◽  
pp. 2361-2366 ◽  
Author(s):  
Fernando J. Kim ◽  
Adriano Campagna ◽  
Lakshmipathi Khandrika ◽  
Sweaty Koul ◽  
Seok-Soo Byun ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Renal Cell ◽  
Individualized Medicine ◽  
Primary Cell ◽  
Primary Cell Line

scholarly journals Metabolomic Analysis to Elucidate Mechanisms of Sunitinib Resistance in Renal Cell Carcinoma

Metabolites ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 1
Author(s):  
Tomonori Sato ◽  
Yoshihide Kawasaki ◽  
Masamitsu Maekawa ◽  
Shinya Takasaki ◽  
Kento Morozumi ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Energy Metabolism ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Progression ◽  
Renal Cell ◽  
Treatment Resistance ◽  
Tca Cycle ◽  
Glutamine Uptake

Metabolomics analysis possibly identifies new therapeutic targets in treatment resistance by measuring changes in metabolites accompanying cancer progression. We previously conducted a global metabolomics (G-Met) study of renal cell carcinoma (RCC) and identified metabolites that may be involved in sunitinib resistance in RCC. Here, we aimed to elucidate possible mechanisms of sunitinib resistance in RCC through intracellular metabolites. We established sunitinib-resistant and control RCC cell lines from tumor tissues of RCC cell (786-O)-injected mice. We also quantified characteristic metabolites identified in our G-Met study to compare intracellular metabolism between the two cell lines using liquid chromatography-mass spectrometry. The established sunitinib-resistant RCC cell line demonstrated significantly desuppressed protein kinase B (Akt) and mesenchymal-to-epithelial transition (MET) phosphorylation compared with the control RCC cell line under sunitinib exposure. Among identified metabolites, glutamine, glutamic acid, and α-KG (involved in glutamine uptake into the tricarboxylic acid (TCA) cycle for energy metabolism); fructose 6-phosphate, D-sedoheptulose 7-phosphate, and glucose 1-phosphate (involved in increased glycolysis and its intermediate metabolites); and glutathione and myoinositol (antioxidant effects) were significantly increased in the sunitinib-resistant RCC cell line. Particularly, glutamine transporter (SLC1A5) expression was significantly increased in sunitinib-resistant RCC cells compared with control cells. In this study, we demonstrated energy metabolism with glutamine uptake and glycolysis upregulation, as well as antioxidant activity, was also associated with sunitinib resistance in RCC cells.

scholarly journals Ghrelin Upregulates Oncogenic Aurora A to Promote Renal Cell Carcinoma Invasion

Cancers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 303 ◽  
Author(s):  
Tsung-Chieh Lin ◽  
Yuan-Ming Yeh ◽  
Wen-Lang Fan ◽  
Yu-Chan Chang ◽  
Wei-Ming Lin ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Growth Hormone ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Renal Cell ◽  
Prognostic Significance ◽  
Dependent Manner ◽  
Aurora A ◽  
Growth Hormone Secretagogue ◽  
Poor Outcome

Ghrelin is a peptide hormone, originally identified from the stomach, that functions as an endogenous ligand of the growth hormone secretagogue receptor (GHSR) and promotes growth hormone (GH) release and food intake. Increasing reports point out ghrelin’s role in cancer progression. We previously characterized ghrelin’s prognostic significance in the clear cell subtype of renal cell carcinoma (ccRCC), and its pro-metastatic ability via Snail-dependent cell migration. However, ghrelin’s activity in promoting cell invasion remains obscure. In this study, an Ingenuity Pathway Analysis (IPA)-based investigation of differentially expressed genes in Cancer Cell Line Encyclopedia (CCLE) dataset indicated the potential association of Aurora A with ghrelin in ccRCC metastasis. In addition, a significant correlation between ghrelin and Aurora A expression level in 15 ccRCC cell line was confirmed by variant probes. ccRCC patients with high ghrelin and Aurora A status were clinically associated with poor outcome. We further observed that ghrelin upregulated Aurora A at the protein and RNA levels and that ghrelin-induced ccRCC in vitro invasion and in vivo metastasis occurred in an Aurora A-dependent manner. Furthermore, MMP1, 2, 9 and 10 expressions are associated with poor outcome. In particular, MMP10 is significantly upregulated and required for the ghrelin-Aurora A axis to promote ccRCC invasion. The results of this study indicated a novel signaling mechanism in ccRCC metastasis.

297 ESTABLISHMENT OF A RENAL CELL CARCINOMA CELL LINE ASSOCIATED WITH TFE3 GENE FUSIONS

2013 ◽  
Vol 189 (4S) ◽  
Author(s):  
Megumi Hirobe ◽  
Naoya Masumori ◽  
Toshiaki Tanaka ◽  
Hiroshi Kitamura ◽  
Taiji Tsukamoto
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Renal Cell ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Gene Fusions ◽  
Renal Cell Carcinoma Cell

Characterization of a Renal Cell Carcinoma Cell Line Suitable as a Target for Immunological Studies in Vitro and in the NU/NU Mouse

1985 ◽  
Vol 134 (6) ◽  
pp. 1271-1275 ◽  
Author(s):  
Martin H. Goldrosen ◽  
Robert Huben ◽  
Glenn A. Miller ◽  
David A. Lewis ◽  
Hisako Ochi ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Renal Cell ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Renal Cell Carcinoma Cell

Spheres derived from the human SK-RC-42 renal cell carcinoma cell line are enriched in cancer stem cells

2010 ◽  
Vol 299 (2) ◽  
pp. 150-160 ◽  
Author(s):  
Yong Zhong ◽  
Kaopeng Guan ◽  
Sujuan Guo ◽  
Chunxia Zhou ◽  
Dongmei Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Renal Cell Carcinoma ◽  
Cancer Stem Cells ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Renal Cell ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Renal Cell Carcinoma Cell
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