Preoxidation Combined with Powdered Activated Carbon and Ultrafiltration to Remove Antibiotic Resistance Genes in Secondary Effluent

2021 ◽  
Author(s):  
Lihua Sun ◽  
Junyao Zhu ◽  
Pengfei Shi ◽  
Yu Ding ◽  
Cuimin Feng
Keyword(s):  
Antibiotic Resistance ◽  
Activated Carbon ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Powdered Activated Carbon ◽  
Secondary Effluent

scholarly journals Removal of antibiotic resistance genes from secondary effluent by processes combining nano-iron, ultrasound-activated persulfate, and ultrafiltration

2021 ◽  
Author(s):  
Lihua Sun ◽  
Hao Tong ◽  
Cheng Gao ◽  
Yehui Liu ◽  
Cuimin Feng
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Sewage Treatment ◽  
Antibiotic Resistance Genes ◽  
Oxidation Mechanism ◽  
Environmental Pollutant ◽  
Secondary Effluent ◽  
Class 1 Integron ◽  
Class 1 ◽  
Activated Persulfate

Abstract Antibiotic resistance genes (ARGs), as a new type of environmental pollutant which threaten human health, have been detected in the effluent of sewage treatment systems. In this study, the removal from water of ARGs, 16S rRNA, class 1 integron (intI1), and dissolved organic carbon (DOC) were investigated using processes combining nano-iron (nFe), ultrasound (US), activated persulfate (PS) and ultrafiltration (UF). The oxidation mechanism was also studied. The results showed that both nFe and US activation could improve the oxidative effect of PS, and the effect of nFe was better than that of US. Compared with PS-UF, nFe/PS-UF and US/PS-UF significantly enhanced the removal of various ARGs and DOC. nFe/PS-UF was the most effective treatment, reducing cell-associated and cell-free ARGs by 1.74–3.14-log and 1.00–2.61-log, respectively, while removing 30% of DOC. Pre-oxidation methods using PS, nFe/PS, and US/PS significantly enhanced the efficacy of UF for removing DOC with molecular weights above 50 kDa and below 10 kDa, but the removal of DOC between 10 and 50 kDa decreased. The free radicals SO4·− and ·OH were shown to participate in the process of ARGs oxidation.

scholarly journals Removal of Antibiotic Resistance Genes at Two Conventional Wastewater Treatment Plants of Louisiana, USA

Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1729 ◽  
Author(s):  
Ocean Thakali ◽  
John P. Brooks ◽  
Shalina Shahin ◽  
Samendra P. Sherchan ◽  
Eiji Haramoto
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Quantitative Polymerase Chain Reaction ◽  
Antibiotic Resistance Genes ◽  
Resistance Mechanisms ◽  
Secondary Effluent ◽  
Integrase Gene ◽  
Final Effluent

Wastewater treatment plants (WWTPs) represent all known types of antibiotic resistance mechanisms and are considered as the critical points for the spread of antibiotic resistance genes (ARGs). The purpose of this study is to investigate the removal of a Class 1 integrase gene (intI1) and a selected set of ARGs (blaTEM, ermF, mecA, and tetA) at two conventional WWTPs by using chlorination in Louisiana, USA. We collected 69 wastewater samples (23 each from influent, secondary effluent, and final effluent) and determined the concentrations of ARGs by using quantitative polymerase chain reaction. All tested ARGs, except for mecA, were detected in 83–96% and 30–65% of influent and final effluent samples, respectively. Although the ARGs underwent approximately 3-log10 reduction, two WWTPs on an average still released 3.3 ± 1.7 log10 copies/mL of total ARGs studied in the effluents. Chlorination was found to be critical in the significant reduction of total ARGs (p < 0.05). Correlation analysis and the ability of intI1 to persist through the treatment processes recommend the use of intI1 as a marker of ARGs in effluents to monitor the spread of antibiotic resistance in effluents. Our study suggests that conventional WWTPs using chlorination do not favor the proliferation of antibiotic resistance bacteria and ARGs during wastewater treatment.

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