scholarly journals Positive Correlation Between the Efficiency of Induced Pluripotent Stem Cells and the Development Rate of Nuclear Transfer Embryos When the Same Porcine Embryonic Fibroblast Lines Are Used As Donor Cells

2014 ◽  
Vol 16 (3) ◽  
pp. 206-214 ◽  
Author(s):  
Bingteng Xie ◽  
Jianyu Wang ◽  
Shichao Liu ◽  
Jiaqiang Wang ◽  
Binghua Xue ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Development Rate ◽  
Positive Correlation ◽  
Donor Cells ◽  
Induced Pluripotent ◽  
Embryonic Fibroblast

scholarly journals Ovine Induced Pluripotent Stem Cells Are Resistant to Reprogramming after Nuclear Transfer

2015 ◽  
Vol 17 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Sergio D. German ◽  
Keith H.S. Campbell ◽  
Elisabeth Thornton ◽  
Gerry McLachlan ◽  
Dylan Sweetman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Induced Pluripotent

scholarly journals Umbilical Cord Tissue as a Source of Young Cells for the Derivation of Induced Pluripotent Stem Cells Using Non-Integrating Episomal Vectors and Feeder-Free Conditions

Cells ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 49
Author(s):  
Aisha Mohamed ◽  
Theresa Chow ◽  
Jennifer Whiteley ◽  
Amanda Fantin ◽  
Kersti Sorra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Umbilical Cord ◽  
Pluripotent Stem Cells ◽  
Blood Cells ◽  
Growth Media ◽  
Donor Cells ◽  
Episomal Vectors ◽  
Umbilical Cord Tissue ◽  
Induced Pluripotent

The clinical application of induced pluripotent stem cells (iPSC) needs to balance the use of an autologous source that would be a perfect match for the patient against any safety or efficacy issues that might arise with using cells from an older patient or donor. Drs. Takahashi and Yamanaka and the Office of Cellular and Tissue-based Products (PMDA), Japan, have had concerns over the existence of accumulated DNA mutations in the cells of older donors and the possibility of long-term negative effects. To mitigate the risk, they have chosen to partner with the Umbilical Cord (UC) banks in Japan to source allogeneic-matched donor cells. Production of iPSCs from UC blood cells (UCB) has been successful; however, reprogramming blood cells requires cell enrichment with columns or flow cytometry and specialized growth media. These requirements add to the cost of production and increase the manipulation of the cells, which complicates the regulatory approval process. Alternatively, umbilical cord tissue mesenchymal stromal cells (CT-MSCs) have the same advantage as UCB cells of being a source of young donor cells. Crucially, CT-MSCs are easier and less expensive to harvest and grow compared to UCB cells. Here, we demonstrate that CT-MSCs can be easily isolated without expensive enzymatic treatment or columns and reprogramed well using episomal vectors, which allow for the removal of the reprogramming factors after a few passages. Together the data indicates that CT-MSCs are a viable source of donor cells for the production of clinical-grade, patient matched iPSCs.

scholarly journals Successful generation of cloned mice using nuclear transfer from induced pluripotent stem cells

Cell Research ◽  
2010 ◽  
Vol 20 (7) ◽  
pp. 850-853 ◽  
Author(s):  
Shuya Zhou ◽  
Chenhui Ding ◽  
Xiaoyang Zhao ◽  
Eryao Wang ◽  
Xiangpeng Dai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Induced Pluripotent

Reprogramming of Somatic Cells After Fusion With Induced Pluripotent Stem Cells and Nuclear Transfer Embryonic Stem Cells

2010 ◽  
Vol 19 (2) ◽  
pp. 239-246 ◽  
Author(s):  
Huseyin Sumer ◽  
Karen L. Jones ◽  
Jun Liu ◽  
Corey Heffernan ◽  
Pollyanna A. Tat ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Somatic Cells ◽  
Embryonic Stem ◽  
Induced Pluripotent

scholarly journals Generation of induced pluripotent stem cells from bovine embryonic fibroblast cells

Cell Research ◽  
2011 ◽  
Vol 21 (10) ◽  
pp. 1509-1512 ◽  
Author(s):  
Xiaoping Han ◽  
Jianyong Han ◽  
Fangrong Ding ◽  
Suying Cao ◽  
Seong Soo Lim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Fibroblast Cells ◽  
Induced Pluripotent ◽  
Embryonic Fibroblast

scholarly journals Establishment of porcine nuclear transfer-derived embryonic stem cells using induced pluripotent stem cells as donor nuclei

2020 ◽  
Vol 66 (2) ◽  
pp. 163-174
Author(s):  
Seiki HARAGUCHI ◽  
Thanh Quang DANG-NGUYEN ◽  
David WELLS ◽  
Daiichiro FUCHIMOTO ◽  
Tomokazu FUKUDA ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Induced Pluripotent

scholarly journals Silencing of retrotransposon-derived imprinted gene RTL1 is the main cause for postimplantational failures in mammalian cloning

2018 ◽  
Vol 115 (47) ◽  
pp. E11071-E11080 ◽  
Author(s):  
Dawei Yu ◽  
Jing Wang ◽  
Huiying Zou ◽  
Tao Feng ◽  
Lei Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Fetal Loss ◽  
Epigenetic Mechanism ◽  
Imprinted Genes ◽  
Pregnancy Failure ◽  
Induced Pluripotent

Substantial rates of fetal loss plague all in vitro procedures involving embryo manipulations, including human-assisted reproduction, and are especially problematic for mammalian cloning where over 90% of reconstructed nuclear transfer embryos are typically lost during pregnancy. However, the epigenetic mechanism of these pregnancy failures has not been well described. Here we performed methylome and transcriptome analyses of pig induced pluripotent stem cells and associated cloned embryos, and revealed that aberrant silencing of imprinted genes, in particular the retrotransposon-derived RTL1 gene, is the principal epigenetic cause of pregnancy failure. Remarkably, restoration of RTL1 expression in pig induced pluripotent stem cells rescued fetal loss. Furthermore, in other mammals, including humans, low RTL1 levels appear to be the main epigenetic cause of pregnancy failure.

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