scholarly journals Enhanced Telomere Rejuvenation in Pluripotent Cells Reprogrammed via Nuclear Transfer Relative to Induced Pluripotent Stem Cells

2014 ◽  
Vol 14 (1) ◽  
pp. 27-39 ◽  
Author(s):  
Rongrong Le ◽  
Zhaohui Kou ◽  
Yonghua Jiang ◽  
Ming Li ◽  
Bo Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Pluripotent Cells ◽  
Induced Pluripotent

scholarly journals Ovine Induced Pluripotent Stem Cells Are Resistant to Reprogramming after Nuclear Transfer

2015 ◽  
Vol 17 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Sergio D. German ◽  
Keith H.S. Campbell ◽  
Elisabeth Thornton ◽  
Gerry McLachlan ◽  
Dylan Sweetman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Induced Pluripotent

scholarly journals Successful generation of cloned mice using nuclear transfer from induced pluripotent stem cells

Cell Research ◽  
2010 ◽  
Vol 20 (7) ◽  
pp. 850-853 ◽  
Author(s):  
Shuya Zhou ◽  
Chenhui Ding ◽  
Xiaoyang Zhao ◽  
Eryao Wang ◽  
Xiangpeng Dai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Induced Pluripotent

Reprogramming of Somatic Cells After Fusion With Induced Pluripotent Stem Cells and Nuclear Transfer Embryonic Stem Cells

2010 ◽  
Vol 19 (2) ◽  
pp. 239-246 ◽  
Author(s):  
Huseyin Sumer ◽  
Karen L. Jones ◽  
Jun Liu ◽  
Corey Heffernan ◽  
Pollyanna A. Tat ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Somatic Cells ◽  
Embryonic Stem ◽  
Induced Pluripotent

scholarly journals 188 MicroRNA characterization in equine induced pluripotent stem cells

2019 ◽  
Vol 31 (1) ◽  
pp. 218
Author(s):  
L. N. Moro ◽  
G. Amin ◽  
V. Furmento ◽  
A. Waisman ◽  
G. Neiman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Seed Region ◽  
Seed Sequence ◽  
Rt Pcr ◽  
Pluripotent Cells ◽  
Mesenchymal Transition ◽  
Mrna Targets ◽  
Induced Pluripotent

Cell reprogramming has been well described in mouse and human cells. The expression of specific microRNAs has demonstrated to be essential for pluripotent maintenance and cell differentiation, but not much information is available in domestic species. A single microRNA can regulate the expression of hundreds of mRNA targets, a property given by a short sequence (called “seed”) in positions 2 to 8 from the 5′ end that is complementary to the 3′ untranslated region (UTR) tail of specific mRNAs. We aimed to generate horse induced pluripotent stem cells (iPSC), characterise them, and evaluate the expression of different microRNAs (miR-302a, b, c, d, miR-205, miR-145, miR-9, miR-96, miR-125b, and miR-296) in pluripotency and differentiation. Both cell states were evaluated (pluripotency and differentiation) in order to understand more deeply the complex network of transcriptional regulation in different contexts but with the same genomic background. Two equine iPSC lines (named L2 and L3) were characterised after the reprogramming of equine fibroblasts with the 4 human Yamanaka factors (OCT-4, SOX-2, c-MYC, KLF4). The pluripotency of both lines was assessed by phosphatase alkaline activity, expression of OCT-4, NANOG, and REX1 by RT-PCR, and by immunofluorescence of OCT-4, SOX-2, and c-MYC. In vitro differentiation to embryo bodies (EB) showed the capacity of the iPSC to differentiate into ectodermal, endodermal, and mesodermal phenotypes. MicroRNA expression was analysed by quantitative RT-PCR and resulted in higher expression of the miR-302 family, miR-9, and miR-96 in L2 and L3v. fibroblasts (P ≤ 0.05), as previously shown in human pluripotent cells. Moreover, down-regulation of miR-145 and miR-205 was observed. After differentiation to EB, greater expression of miR-96 was observed in the EB compared with iPSC, and the expression of miR-205 was induced but only in the EB-L2. In addition, we performed in silico analysis of horse and human microRNAs. First, we compared the horse-miR-302/367 cluster with the human-miR-302/367 cluster and determined a 75% homology between them. Moreover, the seed region of the horse-miR-302 family resulted complementary to the 3′ UTR of horse cell cycle regulator genes CDK2, CYCLIN D1, and E2F1, and to the 3′ UTR of the RHOC gene, which is involved in the epithelial-mesenchymal transition. The miR-145 seed sequence was complementary to the 3′ UTR region of the OCT-4 and KLF-4 horse genes. With respect to miR-9 and miR-96, the seed sequence of these genes were complementary to the HES1 and PAX-6 genes. In all cases, the same gene targets were previously demonstrated in humans. In conclusion, we report the generation and characterization of equine iPSC and determined for the first time the expression of microRNAs in equine pluripotent cells. Moreover, several results led us to think that the horse microRNAs evaluated herein are highly conserved in sequence and function with respect to the human species. It will now be necessary to generate directed differentiations to derivatives of the 3 germ layers in order to strengthen our results. This is the first report to evaluate the expression and possible targets of microRNAs in pluripotent cells from domestic animals.

scholarly journals Human induced pluripotent stem cells display a similar mutation burden as embryonic pluripotent cells in vivo

iScience ◽  
2022 ◽  
pp. 103736
Author(s):  
Karlijn A.L. Hasaart ◽  
Freek Manders ◽  
Joske Ubels ◽  
Mark Verheul ◽  
Markus J. van Roosmalen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Pluripotent Cells ◽  
Mutation Burden ◽  
Induced Pluripotent

scholarly journals Establishment of porcine nuclear transfer-derived embryonic stem cells using induced pluripotent stem cells as donor nuclei

2020 ◽  
Vol 66 (2) ◽  
pp. 163-174
Author(s):  
Seiki HARAGUCHI ◽  
Thanh Quang DANG-NGUYEN ◽  
David WELLS ◽  
Daiichiro FUCHIMOTO ◽  
Tomokazu FUKUDA ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Induced Pluripotent

scholarly journals Silencing of retrotransposon-derived imprinted gene RTL1 is the main cause for postimplantational failures in mammalian cloning

2018 ◽  
Vol 115 (47) ◽  
pp. E11071-E11080 ◽  
Author(s):  
Dawei Yu ◽  
Jing Wang ◽  
Huiying Zou ◽  
Tao Feng ◽  
Lei Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Fetal Loss ◽  
Epigenetic Mechanism ◽  
Imprinted Genes ◽  
Pregnancy Failure ◽  
Induced Pluripotent

Substantial rates of fetal loss plague all in vitro procedures involving embryo manipulations, including human-assisted reproduction, and are especially problematic for mammalian cloning where over 90% of reconstructed nuclear transfer embryos are typically lost during pregnancy. However, the epigenetic mechanism of these pregnancy failures has not been well described. Here we performed methylome and transcriptome analyses of pig induced pluripotent stem cells and associated cloned embryos, and revealed that aberrant silencing of imprinted genes, in particular the retrotransposon-derived RTL1 gene, is the principal epigenetic cause of pregnancy failure. Remarkably, restoration of RTL1 expression in pig induced pluripotent stem cells rescued fetal loss. Furthermore, in other mammals, including humans, low RTL1 levels appear to be the main epigenetic cause of pregnancy failure.

Comparison of Reprogramming Genes in Induced Pluripotent Stem Cells and Nuclear Transfer Cloned Embryos

2014 ◽  
Vol 10 (4) ◽  
pp. 548-560 ◽  
Author(s):  
Lian Duan ◽  
Zhendong Wang ◽  
Jingling Shen ◽  
Zhiyan Shan ◽  
Xinghui Shen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Nuclear Transfer ◽  
Induced Pluripotent
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