scholarly journals Procurement of Human Tissues for Research Banking in the Surgical Pathology Laboratory: Prioritization Practices at Washington University Medical Center

2011 ◽  
Vol 9 (3) ◽  
pp. 245-251 ◽  
Author(s):  
Sandra A. McDonald ◽  
Rebecca D. Chernock ◽  
Tracey A. Leach ◽  
Ajaz A. Kahn ◽  
James H. Yip ◽  
...  
Keyword(s):  
Medical Center ◽  
Surgical Pathology ◽  
Human Tissues ◽  
Pathology Laboratory ◽  
Washington University

Use of FTA Gene Guard Filter Paper for the Storage and Transportation of Tumor Cells for Molecular Testing

2002 ◽  
Vol 126 (1) ◽  
pp. 56-63 ◽  
Author(s):  
Larry J. Dobbs ◽  
Merle N. Madigan ◽  
Alexis B. Carter ◽  
Lori Earls
Keyword(s):  
Tumor Cells ◽  
Filter Paper ◽  
Dna Analysis ◽  
Medical Center ◽  
Surgical Pathology ◽  
Molecular Testing ◽  
Dna Testing ◽  
Pathology Laboratory ◽  
Igh Gene Rearrangement ◽  
Filter Papers

Abstract Context.—Efficient methods of storing tumor specimens for molecular testing are needed in the modern surgical pathology laboratory. The FTA Gene Guard system is a novel method for the collection and room temperature storage of blood samples for DNA testing. The method uses index card–sized filter papers that provide an ideal medium on which to store tumor specimens for DNA testing. Objective.—To determine whether FTA filter paper can be used in the surgical pathology laboratory to store tumor cells for DNA testing. Design.—Cell suspensions were prepared from 60 surgical specimens, and DNA was extracted either immediately or after storage on FTA paper. The DNA extracted by each method was tested by polymerase chain reaction (PCR) for the β-globin and interferon gamma genes, and the results were compared. Fifteen lymph node specimens stored on FTA paper were then tested for immunoglobulin heavy chain (IgH) gene rearrangement by PCR, and these results were compared with those obtained for immediately extracted DNA. Setting.—University medical center. Results.—The DNA extracted from cells stored on FTA paper performed as well in the PCR as the freshly extracted DNA in nearly all cases (>95%). The results of tests for IgH gene rearrangements showed 100% concordance between the 2 methods of DNA extraction. Conclusion.—Cells from surgical specimens can be stored on FTA paper for extended lengths of time, and DNA can be extracted from these cells for PCR-based testing. FTA filter paper is a reliable medium for the storage and/or transport of tumor cells for PCR-based DNA analysis.

Completion Laryngectomy

1979 ◽  
Vol 88 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Ernest M. Myers ◽  
Joseph H. Ogura
Keyword(s):  
Local Recurrence ◽  
Clinical Presentation ◽  
Medical Center ◽  
Partial Laryngectomy ◽  
Respiratory Obstruction ◽  
High Incidence ◽  
Conservation Surgery ◽  
Upper Respiratory ◽  
Washington University ◽  
Salvage Rate

From 1965 to 1975, 452 total laryngectomies were performed at the Washington University Medical Center, St. Louis, Missouri. Forty-two or 9.4% were completion laryngectomies. Completion laryngectomy is defined as the removal of the remaining larynx following an antecedent partial laryngectomy. These 42 cases are retrospectively analyzed in regards to original conservation surgery, clinical presentation, histopathology and salvage rate. The most common indications for completion laryngectomy were: 1) local recurrence, 2) local recurrence with associated severe upper respiratory obstruction, and 3) fistula control. The overall salvage rate following completion laryngectomy was 55% (23 patients). The highest rate was in patients with previous hemilaryngectomy (69%), followed by subtotal supraglottic laryngectomy (44%), and partial laryngopharyngectomy (14%). There is a high incidence of stomal occurrences (24% or 10 patients) associated with completion laryngectomy, particularly in patients with antecedent hemilaryngectomy. Most indications for completion laryngectomy present early, however, all patients must be carefully followed. Both the patient and the otolaryngologist must be ever vigilant following conservation surgery.

scholarly journals Transition to Subspecialty Sign-Out at an Academic Institution and Its Advantages

2017 ◽  
Vol 4 ◽  
pp. 237428951771476 ◽  
Author(s):  
Joanna L. Conant ◽  
Pamela C. Gibson ◽  
Janice Bunn ◽  
Abiy B. Ambaye
Keyword(s):  
Quality Assurance ◽  
Medical Center ◽  
Turnaround Time ◽  
Support Staff ◽  
Surgical Pathology ◽  
Clinician Satisfaction ◽  
Multidisciplinary Conference ◽  
The University ◽  
University Of Vermont ◽  
General Sign

Many pathology departments are introducing subspecialty sign-out in surgical pathology. In 2014, the University of Vermont Medical Center transitioned from general sign-out to partial subspecialty sign-out to include gastrointestinal and breast/cervix subspecialty benches; other specimens remained on general benches. Our experiences with the transition are described, including attending pathologist, trainee, support staff, and clinician satisfaction. A survey was e-mailed to all University of Vermont Medical Center anatomic pathology attendings, pathology trainees, pathologist assistants and grossing technicians, and clinicians who send surgical pathology specimens, immediately before and 1 year after transitioning to partial subspecialty sign-out. Quality assurance metrics were obtained for the 18 months prior to and following the transition. Gastrointestinal and breast/cervix attendings were more satisfied with partial subspecialty sign-out compared to those on the general benches. Overall, trainees were more satisfied with general sign-out because of the rotation schedule but preferred partial subspecialty sign-out due to improved teaching and more focused learning while on subspecialty benches. Clinicians remained very satisfied with our department and our reports; no differences were observed. Turnaround time was unchanged. After switching to partial subspecialty sign-out, there were significantly fewer discrepancies following multidisciplinary conference review for gastrointestinal and breast/cervix cases but remained the same for general cases. Fewer formal internal consults were performed after transitioning to partial subspecialty sign-out across all areas, but more notable for gastrointestinal and breast/cervix cases. Our data show improved quality assurance metrics and trainee education in a subspecialty sign-out setting compared to general sign-out setting.

scholarly journals Analysis of frozen section in correlation with surgical pathology diagnosis

2019 ◽  
Vol 7 (6) ◽  
pp. 2312
Author(s):  
Ashwini Tangde ◽  
Vaidik Shrivastava ◽  
Anil Joshi
Keyword(s):  
Frozen Section ◽  
Final Diagnosis ◽  
Surgical Pathology ◽  
Histopathological Diagnosis ◽  
Pathology Laboratory ◽  
Medical College ◽  
Incorrect Diagnosis ◽  
Frozen Section Diagnosis ◽  
Sensitivity Specificity ◽  
Suspected Lesion

Background: Frozen section (FS) is a rapid diagnostic procedure performed on tissues obtained intraoperatively. This method serves useful purposes, such as determining the malignancy or benignancy of a suspected lesion, determining the adequacy of a biopsy of a suspected lesion, confirming the presence or absence of metastasis, and identifying small structures. But it bears many disadvantages and limitations, the most of which is the danger of incorrect diagnosis. Therefore, it is critical to determine efficiency of frozen section performance periodically.  This study was performed to determine accuracy of frozen section by correlating the intra-operative frozen section diagnosis with final diagnosis on permanent sections.Methods: In this retrospective study, authors compared the results of frozen section with their final permanent section diagnosis in Government Medical College and Hospital, Aurangabad, Maharashtra, India during January 2017 to December 2018.Results: Study comprises 83 patients, of which 73 were female and 10 were male. Out of 83 cases, the diagnosis of 76 cases was concordant with conventional histopathology diagnosis while seven were discordant. This gave overall accuracy rate of 91.57% and discordant rate of 8.43%. The overall sensitivity was 85.71% and specificity was 97.92%. The positive predictive and negative predictive value was 96.77% and 90.38% respectively.Conclusions: The accuracy, sensitivity, specificity of frozen section diagnosis in this study  are comparable with most international quality control statistics for frozen sections. The results suggest that the correlation of intra-operative frozen section diagnosis with the final histopathological diagnosis on permanent sections forms an integral part of quality assurance activities in the surgical pathology laboratory and specific measures should be taken to reduce the number of discrepancies.

Virtual Surgical Pathology in Underdeveloped Countries: The Zambia Project

2011 ◽  
Vol 135 (2) ◽  
pp. 215-219
Author(s):  
Fabio Pagni ◽  
Francesca Bono ◽  
Camillo Di Bella ◽  
Agostino Faravelli ◽  
Anna Cappellini
Keyword(s):  
Developing Country ◽  
Microscopic Examination ◽  
Surgical Pathology ◽  
Virtual Laboratory ◽  
The Internet ◽  
Pathology Laboratory ◽  
Italian Association ◽  
Underdeveloped Countries ◽  
Mission Hospital

Abstract Only 1 surgical pathology laboratory is available in Zambia, a country with a population of 12 million people. Since 2004 the Italian association of pathologists Patologi Oltre Frontiera has been working to create a virtual laboratory through the use of telemedicine. The project has involved staining histologic preparations on site, with the interpretation of imaged slides performed abroad through telepathology. Starting in April 2007, all surgical specimens obtained in Mtendere Mission Hospital, Chirundu, Zambia, were submitted for microscopic examination through whole-slide scans. Two independent Italian pathologists evaluated the cases by means of satellite connection and the final diagnoses were sent to Zambian clinicians via the internet. This article describes the spectrum of diagnoses made via telepathology for the Zambian population. Also, we analyze the concordant and discordant data between this telepathology method and traditional microscopy in a developing country. Moreover, we provide possible solutions for providing pathology services in other underdeveloped countries.

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