scholarly journals Regulatory T Cell Effect on CD8+T Cell Responses to Human Herpesvirus 8 Infection and Development of Kaposi's Sarcoma

2017 ◽  
Vol 33 (7) ◽  
pp. 668-674 ◽  
Author(s):  
Lauren M. Lepone ◽  
Giovanna Rappocciolo ◽  
Paolo A. Piazza ◽  
Diana M. Campbell ◽  
Frank J. Jenkins ◽  
...  
Keyword(s):  
T Cell ◽  
Regulatory T Cell ◽  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
T Cell Responses ◽  
Kaposi’S Sarcoma ◽  
Cd8 T Cell ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Cell Responses

scholarly journals CD4 regulatory T cells Control CD8 T cell responses to human Herpesvirus 8 lytic and latency proteins

2012 ◽  
Vol 7 (S1) ◽  
Author(s):  
Lauren Lepone ◽  
Giovanna Rappocciolo ◽  
Paolo Piazza ◽  
Mariel Jais ◽  
Frank J Jenkins ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Regulatory T Cells ◽  
Human Herpesvirus ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Cell Responses

Seroreactivity to Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) latent nuclear antigen in AIDS-associated Kaposi's sarcoma patients depends on CD4+ T-cell count

2007 ◽  
Vol 79 (10) ◽  
pp. 1562-1568 ◽  
Author(s):  
Vanda Akico Ueda Fick de Souza ◽  
Ligia Camera Pierrotti ◽  
Laura Masami Sumita ◽  
Wilton Santos Freire ◽  
Aluisio Augusto Cotrim Segurado ◽  
...  
Keyword(s):  
T Cell ◽  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Cd4 T Cell ◽  
Human Herpesvirus 8 ◽  
Nuclear Antigen ◽  
Herpesvirus 8 ◽  
Latent Nuclear Antigen ◽  
Cd4 T Cell Count

scholarly journals Monofunctional and Polyfunctional CD8+ T Cell Responses to Human Herpesvirus 8 Lytic and Latency Proteins

2010 ◽  
Vol 17 (10) ◽  
pp. 1507-1516 ◽  
Author(s):  
Lauren Lepone ◽  
Giovanna Rappocciolo ◽  
Emilee Knowlton ◽  
Mariel Jais ◽  
Paolo Piazza ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Primary Effusion Lymphoma ◽  
Interleukin 2 ◽  
Human Herpesvirus ◽  
T Cell Responses ◽  
Human Herpesvirus 8 ◽  
Nuclear Antigen ◽  
Herpesvirus 8 ◽  
Cell Responses

ABSTRACT Human herpesvirus 8 (HHV-8) is the etiological agent of Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. It is postulated that CD8+ T cell responses play an important role in controlling HHV-8 infection and preventing development of disease. In this study, we investigated monofunctional and polyfunctional CD8+ T cell responses to HHV-8 lytic proteins gB (glycoprotein B) and K8.1 and latency proteins LANA-1 (latency-associated nuclear antigen-1) and K12. On the basis of our previous findings that dendritic cells (DC) reveal major histocompatibility complex (MHC) class I epitopes in gB, we used a DC-based system to identify 2 novel epitopes in gB, 2 in K8.1, 5 in LANA-1, and 1 in K12. These new HHV-8 epitopes activated monofunctional and polyfunctional CD8+ T cells that produced various combinations of gamma interferon, interleukin 2, tumor necrosis factor alpha, macrophage inhibitory protein 1β, and cytotoxic degranulation marker CD107a in healthy HHV-8-seropositive individuals. We were also able to detect HHV-8-specific CD8+ T cells in peripheral blood samples using HLA A*0201 pentamer complexes for one gB epitope, one K8.1 epitope, two LANA-1 epitopes, and one K12 epitope. These immunogenic regions of viral lytic and latency proteins could be important in T cell control of HHV-8 infection.

scholarly journals Low T Cell Responses to Human Herpesvirus 8 in Patients with AIDS‐Related and Classic Kaposi Sarcoma

2006 ◽  
Vol 194 (8) ◽  
pp. 1078-1088 ◽  
Author(s):  
Amélie Guihot ◽  
Nicolas Dupin ◽  
Anne‐Geneviève Marcelin ◽  
Isabelle Gorin ◽  
Anne‐Sophie Bedin ◽  
...  
Keyword(s):  
T Cell ◽  
Human Herpesvirus ◽  
Kaposi Sarcoma ◽  
T Cell Responses ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Cell Responses ◽  
Classic Kaposi Sarcoma

Detection of human herpesvirus 8 DNA and antibodies to latent nuclear and lytic-phase antigens in serial samples from aids patients with Kaposi’s sarcoma

2000 ◽  
Vol 16 (3) ◽  
pp. 247-251 ◽  
Author(s):  
Lı́gia Camera Pierrotti ◽  
Laura Masami Sumita ◽  
Wilton Santos Freire ◽  
Hélio Hehl Caiaffa Filho ◽  
Vanda Akico Ueda Fick de Souza
Keyword(s):  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Aids Patients ◽  
Herpesvirus 8 ◽  
Serial Samples

scholarly journals Activation of NF-κB by the Human Herpesvirus 8 Chemokine Receptor ORF74: Evidence for a Paracrine Model of Kaposi's Sarcoma Pathogenesis

2001 ◽  
Vol 75 (18) ◽  
pp. 8660-8673 ◽  
Author(s):  
Shibani Pati ◽  
Marielle Cavrois ◽  
Hong-Guang Guo ◽  
James S. Foulke ◽  
Jynho Kim ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Kaposi's Sarcoma ◽  
Vascular Endothelial Cells ◽  
Inositol Phosphate ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Lymphoid Cells ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Latently Infected

ABSTRACT Infection with human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma (KS)-associated herpesvirus, is necessary for the development of KS. The HHV-8 lytic-phase gene ORF74 is related to G protein-coupled receptors, particularly interleukin-8 (IL-8) receptors. ORF74 activates the inositol phosphate/phospholipase C pathway and the downstream mitogen-activated protein kinases, JNK/SAPK and p38. We show here that ORF74 also activates NF-κB independent of ligand when expressed in KS-derived HHV-8-negative endothelial cells or primary vascular endothelial cells. NF-κB activation was enhanced by the chemokine GROα, but not by IL-8. Mutation of Val to Asp in the ORF74 second cytoplasmic loop did not affect ligand-independent signaling activity, but it greatly increased the response to GROα. ORF74 upregulated the expression of NF-κB-dependent inflammatory cytokines (RANTES, IL-6, IL-8, and granulocyte-macrophage colony-stimulating factor) and adhesion molecules (VCAM-1, ICAM-1, and E-selectin). Supernatants from transfected KS cells activated NF-κB signaling in untransfected cells and elicited the chemotaxis of monocytoid and T-lymphoid cells. Expression of ORF74 conferred on primary endothelial cells a morphology that was strikingly similar to that of spindle cells present in KS lesions. Taken together, these data, demonstrating that ORF74 activates NF-κB and induces the expression of proangiogenic and proinflammatory factors, suggest that expression of ORF74 in a minority of cells in KS lesions could influence uninfected cells or latently infected cells via autocrine and paracrine mechanisms, thereby contributing to KS pathogenesis.

scholarly journals Lytic and Latent Antigens of the Human Gammaherpesviruses Kaposi's Sarcoma-Associated Herpesvirus and Epstein-Barr Virus Induce T-Cell Responses with Similar Functional Properties and Memory Phenotypes

2007 ◽  
Vol 81 (9) ◽  
pp. 4904-4908 ◽  
Author(s):  
Florian Bihl ◽  
Murli Narayan ◽  
John V. Chisholm ◽  
Leah M. Henry ◽  
Todd J. Suscovich ◽  
...  
Keyword(s):  
T Cell ◽  
Cellular Immunity ◽  
Kaposi's Sarcoma ◽  
Epstein Barr Virus ◽  
T Cell Responses ◽  
Kaposi’S Sarcoma ◽  
Barr Virus ◽  
Cell Responses ◽  
Epstein Barr ◽  
Kaposi’S Sarcoma Associated Herpesvirus

ABSTRACT The cellular immunity against Kaposi's sarcoma-associated herpesvirus (KSHV) is poorly characterized and has not been compared to T-cell responses against other human herpesviruses. Here, novel and dominant targets of KSHV-specific cellular immunity are identified and compared to T cells specific for lytic and latent antigens in a second human gammaherpesvirus, Epstein-Barr virus. The data identify a novel HLA-B57- and HLA-B58-restricted epitope in the Orf57 protein and show consistently close parallels in immune phenotypes and functional response patterns between cells targeting lytic or latent KSHV- and EBV-encoded antigens, suggesting common mechanisms in the induction of these responses.

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