The Use of High-Content Screening for the Discovery and Characterization of Compounds That Modulate Mitotic Index and Cell Cycle Progression by Differing Mechanisms of Action

2006 ◽  
Vol 4 (2) ◽  
pp. 153-163 ◽  
Author(s):  
Amy Barabasz ◽  
Briana Foley ◽  
James C. Otto ◽  
Anisa Scott ◽  
John Rice
Keyword(s):  
Cell Cycle ◽  
Mitotic Index ◽  
Cell Cycle Progression ◽  
Mechanisms Of Action ◽  
High Content Screening ◽  
Cycle Progression

scholarly journals Toward Discovery of Novel Microtubule Targeting Agents: A SNAP-tag–Based High-Content Screening Assay for the Analysis of Microtubule Dynamics and Cell Cycle Progression

2017 ◽  
Vol 22 (4) ◽  
pp. 387-398
Author(s):  
Nina Berges ◽  
Katharina Arens ◽  
Verena Kreusch ◽  
Rainer Fischer ◽  
Stefano Di Fiore
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
A549 Cells ◽  
Reactive Dye ◽  
High Content Screening ◽  
Screening Assay ◽  
Cycle Progression ◽  
Microtubule Targeting Agents ◽  
Automated Microscopy

Microtubule targeting agents (MTAs) are used for the treatment of cancer. Novel MTAs could provide additional and beneficial therapeutic options. To improve the sensitivity and throughput of standard immunofluorescence assays for the characterization of MTAs, we used SNAP-tag technology to produce recombinant tubulin monomers. To visualize microtubule filaments, A549 cells transfected with SNAP-tubulin were stained with a membrane-permeable, SNAP-reactive dye. The treatment of SNAP-tubulin cells with stabilizing MTAs such as paclitaxel resulted in the formation of coarsely structured microtubule filaments, whereas depolymerizing MTAs such as nocodazole resulted in diffuse staining patterns in which the tubulin filaments were no longer distinguishable. By combining these components with automated microscopy and image analysis algorithms, we established a robust high-content screening assay for MTAs with a Z′ factor of 0.7. Proof of principle was achieved by testing a panel of 10 substances, allowing us to identify MTAs and to distinguish between stabilizing and destabilizing modes of action. By extending the treatment of the cells from 2 to 20 h, our assay also detected abnormalities in cell cycle progression and in the formation of microtubule spindles, providing additional readouts for the discovery of new MTAs and facilitating their early identification during drug-screening campaigns.

Abstract LB-159: Characterization of cell-cycle progression using a novel bi-cistronic lentiviral FUCCI reporter

2014 ◽  
Author(s):  
Gabriel Pineda ◽  
Florence Lambert-Fliszar ◽  
Gennarina L. Riso ◽  
Kathleen M. Kane ◽  
Catriona Jamieson
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Cycle Progression

scholarly journals Mammalian MYC Proteins and Cancer

2014 ◽  
Vol 2014 ◽  
pp. 1-27 ◽  
Author(s):  
William P. Tansey
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Cycle Progression ◽  
Leucine Zipper ◽  
Mechanisms Of Action ◽  
Cycle Progression ◽  
Oncogenic Potential ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix ◽  
Growth Metabolism

The MYC family of proteins is a group of basic-helix-loop-helix-leucine zipper transcription factors that feature prominently in cancer. Overexpression of MYC is observed in the vast majority of human malignancies and promotes an extraordinary set of changes that impact cell proliferation, growth, metabolism, DNA replication, cell cycle progression, cell adhesion, differentiation, and metastasis. The purpose of this review is to introduce the reader to the mammalian family of MYC proteins, highlight important functional properties that endow them with their potent oncogenic potential, describe their mechanisms of action and of deregulation in cancer cells, and discuss efforts to target the unique properties of MYC, and of MYC-driven tumors, to treat cancer.

scholarly journals Multi-Modal Fluorescence Characterization of Cell Cycle Progression and Cytokinesis

2019 ◽  
Vol 116 (3) ◽  
pp. 24a
Author(s):  
Rachel Cinco ◽  
Per Niklas Hedde ◽  
Leonel Malacrida ◽  
Michelle A. Digman ◽  
Enrico Gratton
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Cycle Progression

scholarly journals Characterization of the effects of cisplatin and carboplatin on cell cycle progression and DNA damage response activation in DNA polymerase eta-deficient human cells

Cell Cycle ◽  
2009 ◽  
Vol 8 (18) ◽  
pp. 3043-3054 ◽  
Author(s):  
Séverine Cruet-Hennequart ◽  
Sangamitra Villalan ◽  
Agnieszka Kaczmarczyk ◽  
Elaine O’Meara ◽  
Anna M. Sokol ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Polymerase ◽  
Dna Damage Response ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
Polymerase Eta ◽  
Dna Polymerase Eta ◽  
Damage Response ◽  
Response Activation
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