Molecular characterization of newly identified Klebsiella PKBSG14 and analysis of its effect on immune response and cell cycle progression using common catfish (Channa punctatus) as a model

2019 ◽  
Vol 127 ◽  
pp. 368-379 ◽  
Author(s):  
Subarna Ghosh ◽  
P.K. Bandyopadhyay
Keyword(s):  
Cell Cycle ◽  
Immune Response ◽  
Molecular Characterization ◽  
Cell Cycle Progression ◽  
Channa Punctatus ◽  
Cycle Progression

scholarly journals Rapid proliferation of activated lymph node CD4+ T cells is achieved by greatly curtailing the duration of gap phases in cell cycle progression

2014 ◽  
Vol 19 (4) ◽  
Author(s):  
Takuya Mishima ◽  
Shoko Toda ◽  
Yoshiaki Ando ◽  
Tsukasa Matsunaga ◽  
Manabu Inobe
Keyword(s):  
Cell Cycle ◽  
Immune Response ◽  
T Cells ◽  
Cd4 T Cells ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
Cycle Times ◽  
Peripheral T Cells ◽  
G0 Phase

AbstractPeripheral T cells are in G0 phase and do not proliferate. When they encounter an antigen, they enter the cell cycle and proliferate in order to initiate an active immune response. Here, we have determined the first two cell cycle times of a leading population of CD4+ T cells stimulated by PMA plus ionomycin in vitro. The first cell cycle began around 10 h after stimulation and took approximately 16 h. Surprisingly, the second cell cycle was extremely rapid and required only 6 h. T cells might have a unique regulatory mechanism to compensate for the shortage of the gap phases in cell cycle progression. This unique feature might be a basis for a quick immune response against pathogens, as it maximizes the rate of proliferation.

scholarly journals IFNγ suppresses the expression of GFI1 and thereby inhibits Th2 cell proliferation

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260204
Author(s):  
Murshed H. Sarkar ◽  
Ryoji Yagi ◽  
Yukihiro Endo ◽  
Ryo Koyama-Nasu ◽  
Yangsong Wang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Immune Response ◽  
Cell Proliferation ◽  
Cell Cycle Progression ◽  
Th2 Cells ◽  
Type I ◽  
Type Ii ◽  
Cycle Progression ◽  
Th2 Cell ◽  
Differentiation And Proliferation

While IFNγ is a well-known cytokine that actively promotes the type I immune response, it is also known to suppress the type II response by inhibiting the differentiation and proliferation of Th2 cells. However, the mechanism by which IFNγ suppresses Th2 cell proliferation is still not fully understood. We found that IFNγ decreases the expression of growth factor independent-1 transcriptional repressor (GFI1) in Th2 cells, resulting in the inhibition of Th2 cell proliferation. The deletion of the Gfi1 gene in Th2 cells results in the failure of their proliferation, accompanied by an impaired cell cycle progression. In contrast, the enforced expression of GFI1 restores the defective Th2 cell proliferation, even in the presence of IFNγ. These results demonstrate that GFI1 is a key molecule in the IFNγ-mediated inhibition of Th2 cell proliferation.

Abstract LB-159: Characterization of cell-cycle progression using a novel bi-cistronic lentiviral FUCCI reporter

2014 ◽  
Author(s):  
Gabriel Pineda ◽  
Florence Lambert-Fliszar ◽  
Gennarina L. Riso ◽  
Kathleen M. Kane ◽  
Catriona Jamieson
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Cycle Progression

scholarly journals Relevance of BET Family Proteins in SARS-CoV-2 Infection

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1126
Author(s):  
Nieves Lara-Ureña ◽  
Mario García-Domínguez
Keyword(s):  
Cell Cycle ◽  
Immune Response ◽  
Animal Models ◽  
Cell Cycle Progression ◽  
Structural Proteins ◽  
Transcription Control ◽  
Cycle Progression ◽  
E Protein ◽  
Different Types ◽  
New Type

The recent pandemic we are experiencing caused by the coronavirus disease 2019 (COVID-19) has put the world’s population on the rack, with more than 191 million cases and more than 4.1 million deaths confirmed to date. This disease is caused by a new type of coronavirus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A massive proteomic analysis has revealed that one of the structural proteins of the virus, the E protein, interacts with BRD2 and BRD4 proteins of the Bromodomain and Extra Terminal domain (BET) family of proteins. BETs are essential to cell cycle progression, inflammation and immune response and have also been strongly associated with infection by different types of viruses. The fundamental role BET proteins play in transcription makes them appropriate targets for the propagation strategies of some viruses. Recognition of histone acetylation by BET bromodomains is essential for transcription control. The development of drugs mimicking acetyl groups, and thereby able to displace BET proteins from chromatin, has boosted interest on BETs as attractive targets for therapeutic intervention. The success of these drugs against a variety of diseases in cellular and animal models has been recently enlarged with promising results from SARS-CoV-2 infection studies.

scholarly journals Toward Discovery of Novel Microtubule Targeting Agents: A SNAP-tag–Based High-Content Screening Assay for the Analysis of Microtubule Dynamics and Cell Cycle Progression

2017 ◽  
Vol 22 (4) ◽  
pp. 387-398
Author(s):  
Nina Berges ◽  
Katharina Arens ◽  
Verena Kreusch ◽  
Rainer Fischer ◽  
Stefano Di Fiore
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
A549 Cells ◽  
Reactive Dye ◽  
High Content Screening ◽  
Screening Assay ◽  
Cycle Progression ◽  
Microtubule Targeting Agents ◽  
Automated Microscopy

Microtubule targeting agents (MTAs) are used for the treatment of cancer. Novel MTAs could provide additional and beneficial therapeutic options. To improve the sensitivity and throughput of standard immunofluorescence assays for the characterization of MTAs, we used SNAP-tag technology to produce recombinant tubulin monomers. To visualize microtubule filaments, A549 cells transfected with SNAP-tubulin were stained with a membrane-permeable, SNAP-reactive dye. The treatment of SNAP-tubulin cells with stabilizing MTAs such as paclitaxel resulted in the formation of coarsely structured microtubule filaments, whereas depolymerizing MTAs such as nocodazole resulted in diffuse staining patterns in which the tubulin filaments were no longer distinguishable. By combining these components with automated microscopy and image analysis algorithms, we established a robust high-content screening assay for MTAs with a Z′ factor of 0.7. Proof of principle was achieved by testing a panel of 10 substances, allowing us to identify MTAs and to distinguish between stabilizing and destabilizing modes of action. By extending the treatment of the cells from 2 to 20 h, our assay also detected abnormalities in cell cycle progression and in the formation of microtubule spindles, providing additional readouts for the discovery of new MTAs and facilitating their early identification during drug-screening campaigns.

scholarly journals Multi-Modal Fluorescence Characterization of Cell Cycle Progression and Cytokinesis

2019 ◽  
Vol 116 (3) ◽  
pp. 24a
Author(s):  
Rachel Cinco ◽  
Per Niklas Hedde ◽  
Leonel Malacrida ◽  
Michelle A. Digman ◽  
Enrico Gratton
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Cycle Progression
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