Autonomic Responses to Ultrasound-Guided Percutaneous Needle Electrolysis: Effect of Needle Puncture or Electrical Current?

2018 ◽  
Vol 24 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Paula García Bermejo ◽  
Blanca De La Cruz Torres ◽  
José Naranjo Orellana ◽  
Manuel Albornoz Cabello
Keyword(s):  
Electrical Current ◽  
Needle Puncture ◽  
Ultrasound Guided ◽  
Autonomic Responses

scholarly journals IN UTERO TRANSPLANTATION OF HUMAN STEM CELLS USING AN ANIMAL MODEL: A COMPARISON BETWEEN TWO TECHNIQUES

2009 ◽  
Vol 15 (1) ◽  
pp. 5-10
Author(s):  
Daria GROZA ◽  
Emoke PALL ◽  
Mihai CENARIU ◽  
Raul POP ◽  
Nicolae Nicolae ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Loss Rate ◽  
Fetal Loss ◽  
In Utero ◽  
Needle Puncture ◽  
Ultrasound Guided ◽  
In Utero Transplantation ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

Objective: The purpose of this study was to assess the feasibility of in utero stem cell transplantation of human umbilical cord blood stem cells in fetal sheep and to compare two different techniques of in utero transplantation, namely ultrasound-guided in utero transplantation and in utero transplantation after midline celiotomy. Study design: Umbilical cord blood units were collected from term deliveries, after obtaining written informed consent. Human cord blood–derived, CD34+ stem cells were injected into the peritoneal cavity of 60- to 65-day-old ovine fetuses by using 2 different techniques: ultrasound-guided transabdominal percutaneous needle puncture and midline celiotomy with the exposure of the pregnant uterus. Engraftment was determined after birth by flow cytometry with use of human-specific anti-CD 34/45 antibodies. Results: We obtained a total of 3 chimeric lambs. Using the midline celiotomy technique the fetal loss rate was 75% and only 33,3% when using ultrasound-guided transabdominal percutaneous needle puncture technique. Engraftment of donor cells was found in all fetuses, with a mean level of 1.4% in fetal peripheral blood and 3.3% in fetal bone marrow. Conclusion: This preliminary study indicates that in utero stem cell transplantation of human hematopoietic cord blood stem cells in fetal lambs is feasible and effective in terms of hematopoietic engraftment. We also concluded that the ultrasound-guided transabdominal percutaneous needle puncture technique is more effective than performing a midline celiotomy in terms of fetal loss rate.

Ultrasound-guided percutaneous fine needle puncture of the gallbladder for studies of bile composition

1995 ◽  
Vol 68 (807) ◽  
pp. 271-276 ◽  
Author(s):  
S H Hussaini ◽  
C Kennedy ◽  
S P Pereira ◽  
J A H Wass ◽  
R H Dowling
Keyword(s):  
Needle Puncture ◽  
Ultrasound Guided ◽  
Fine Needle ◽  
Bile Composition ◽  
Fine Needle Puncture

Endobronchial ultrasound-guided transbronchial needle aspiration samples for multiple molecular analysis.

2012 ◽  
Vol 30 (30_suppl) ◽  
pp. 75-75
Author(s):  
Kezhong Chen ◽  
Fan Yang ◽  
Hui Zhao ◽  
Jun Wang
Keyword(s):  
Molecular Analysis ◽  
Mediastinal Lymph Node ◽  
Needle Aspiration ◽  
Endobronchial Ultrasound ◽  
Egfr Mutations ◽  
Amplification Refractory Mutation System ◽  
Needle Puncture ◽  
Ultrasound Guided ◽  
Transbronchial Needle Aspiration ◽  
Egfr Gene

75 Background: We assessed the usefulness of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) to detect EGFR, K-ras, ALK mutations from malignant mediastinal nodes of patients with NSCLC. Methods: From February 2010 to April 2012, 81 specimens diagnosed with lung adenocarcinoma by EBUS were used for molecular analysis. Needle puncture site for the EBUS was standard 22G needle, once cytology pathology diagnosed adenocarcinoma, at the same site with the conventional needle puncture method to obtain adequate biopsy specimen for further analysis. The tissue was fixed in 10% formalin. When histologic diagnosis confirmed adenocarcinoma, the FFPE tissue was extracted DNA to detect EGFR mutation by both amplification refractory mutation system (ARMS) and direct sequencing. Partial specimens also extracted DNA or RNA to detect K-ras and ALK mutation. Results: 80/81 specimens were successfully extracted DNA, 52 were male and 28 were female, mean age of 62.9 years (34 to 82 years). Somatic mutations of the EGFR gene were detected in 37 of 80 cases (46.3%), 22 cases of exon 19 mutation,14 cases of exon 21 mutation, and 1 of T790M mutation in ARMS. The result have some difference in direct sequening. In the patients who requested other biomarkers analysis, 6 had K-ras mutations and 2 had ALK mutations.7 patients with EGFR mutations had a radical surgery after neoadjuvant chemotherapy, tumor specimens were tested again with EGFR gene mutation, the same result as the preoperative test results. Conclusions: EBUS-TBNA mediastinal lymph node biopsy in the detection of EGFR, K-ras, and ALK mutations in tissue samples was feasible, through the highly sensitive methods, higher detection rate was available, the result can be used as evidence of personalized treatment of lung cancer.

Recurrent formation of haematocolpos in a young girl with multiple congenital anomalies of the urogenital tract

2021 ◽  
pp. 1-7
Author(s):  
Mehreen Yousaf Rana ◽  
Syeda Hira Naqvi ◽  
Rubina Hussain
Keyword(s):  
Needle Puncture ◽  
Multiple Congenital Anomalies ◽  
Ultrasound Guided ◽  
Imperforate Hymen ◽  
Incision And Drainage ◽  
Renal Ectopia ◽  
Urogenital Abnormalities ◽  
Vaginal Orifice ◽  
Crossed Fused Renal Ectopia ◽  
Definitive Management

Abstract An 11-year-old girl, a known case of left crossed fused renal ectopia and sacral hypoplasia presented to the gynaecological OPD in Karachi, Pakistan, in February 2019 with complaints of abdominal pain. On examination, she was found to have a septum covering her vaginal orifice. She was subsequently diagnosed with haematocolpos secondary to imperforate hymen. Incision and drainage was done. However, despite surgical management, she continued to have recurrent formation of haematocolpos for the next two months secondary to multiple complete and partial transverse vaginal septa and post-operative formation of adhesions. Definitive management was done with ultrasound guided needle puncture and drainage, followed by post-operative tampon use to maintain patency. Keywords: Haematocolpos, urogenital abnormalities, Continuous...

Evaluating the long-term effect of ultrasound-guided needle puncture without aspiration on calcifying supraspinatus tendinitis

2008 ◽  
Vol 25 (11) ◽  
pp. 1229-1234 ◽  
Author(s):  
Jiaan Zhu ◽  
Yeqing Jiang ◽  
Yizhou Hu ◽  
Chunyan Xing ◽  
Bing Hu
Keyword(s):  
Term Effect ◽  
Needle Puncture ◽  
Ultrasound Guided ◽  
Long Term Effect ◽  
Supraspinatus Tendinitis
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