HIV Type 1 Subtype E-Infected Patients with Broadened, Dual (B/E) V3 Loop Serology Have Increased Cross-Neutralizing Antibodies

2001 ◽  
Vol 17 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Victoria R. Polonis ◽  
Mark S. de Souza ◽  
Penprapa Chanbancherd ◽  
Somsak Chantakulkij ◽  
Achara Jugsudee ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
V3 Loop ◽  
Hiv Type 1

scholarly journals Unique V3 Loop Sequence Derived from the R2 Strain of HIV-Type 1 Elicits Broad Neutralizing Antibodies

2004 ◽  
Vol 20 (11) ◽  
pp. 1259-1268 ◽  
Author(s):  
Kelly R. Young ◽  
Benjamin E. Teal ◽  
Yvonne Brooks ◽  
Thomas D. Green ◽  
Joseph F. Bower ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
V3 Loop ◽  
Loop Sequence ◽  
Hiv Type 1

Contrasting IgA and IgG Neutralization Capacities and Responses to HIV Type 1 gp120 V3 Loop in HIV-Infected Individuals

1994 ◽  
Vol 10 (7) ◽  
pp. 813-822 ◽  
Author(s):  
PAMELA A. KOZLOWSKI ◽  
DEXIANG CHEN ◽  
JOHN H. ELDRIDGE ◽  
SUSAN JACKSON
Keyword(s):  
V3 Loop ◽  
Hiv Type 1

scholarly journals Structural Conservation Predominates Over Sequence Variability in the Crown of HIV Type 1's V3 Loop

2010 ◽  
Vol 26 (6) ◽  
pp. 717-723 ◽  
Author(s):  
David Almond ◽  
Tetsuya Kimura ◽  
XiangPeng Kong ◽  
James Swetnam ◽  
Susan Zolla-Pazner ◽  
...  
Keyword(s):  
V3 Loop ◽  
Sequence Variability ◽  
Hiv Type 1 ◽  
Structural Conservation

Short Communication: In VitroSynergy between Peptides or Neutralizing Antibodies Targeting the N- and C-Terminal Heptad Repeats of HIV Type 1 gp41

2008 ◽  
Vol 24 (12) ◽  
pp. 1537-1544 ◽  
Author(s):  
Renee Hrin ◽  
Donna L. Montgomery ◽  
Fubao Wang ◽  
Jon H. Condra ◽  
Zhiqiang An ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Short Communication ◽  
Hiv Type 1 ◽  
Heptad Repeats

scholarly journals Selective Modification of Variable Loops Alters Tropism and Enhances Immunogenicity of Human Immunodeficiency Virus Type 1 Envelope

2004 ◽  
Vol 78 (8) ◽  
pp. 4029-4036 ◽  
Author(s):  
Zhi-yong Yang ◽  
Bimal K. Chakrabarti ◽  
Ling Xu ◽  
Brent Welcher ◽  
Wing-pui Kong ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Neutralizing Antibodies ◽  
Neutralizing Antibody ◽  
Amino Acid Sequences ◽  
V3 Loop ◽  
Variable Regions ◽  
Immunodeficiency Virus

ABSTRACT Although the B clade of human immunodeficiency virus type 1 (HIV-1) envelopes (Env) includes five highly variable regions, each of these domains contains a subset of sequences that remain conserved. The V3 loop has been much studied for its ability to elicit neutralizing antibodies, which are often restricted to a limited number of closely related strains, likely because a large number of antigenic structures are generated from the diverse amino acid sequences in this region. Despite these strain-specific determinants, subregions of V3 are highly conserved, and the effects of different portions of the V3 loop on Env tropism and immunogenicity have not been well delineated. For this report, selective deletions in V3 were introduced by shortening of the stem of the V3 loop. These mutations were explored in combination with deletions of selected V regions. Progressive shortening of the stem of V3 abolished the immunogenicity as well as the functional activity of HIV Env; however, two small deletions on both arms of the V3 stem altered the tropism of the dualtropic 89.6P viral strain so that it infected only CXCR4+ cells. When this smaller deletion was combined with removal of the V1 and V2 loops and used as an immunogen in guinea pigs, the antisera were able to neutralize multiple independent clade B isolates with a higher potency. These findings suggest that highly conserved subregions within V3 may be relevant targets for eliciting neutralizing antibody responses, affecting HIV tropism, and increasing the immunogenicity of AIDS vaccines.

scholarly journals Enhancement of human immunodeficiency virus type 1 infection by antisera to peptides from the envelope glycoproteins gp120/gp41.

1991 ◽  
Vol 174 (6) ◽  
pp. 1557-1563 ◽  
Author(s):  
S B Jiang ◽  
K Lin ◽  
A R Neurath
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Neutralizing Antibodies ◽  
Rational Design ◽  
Envelope Glycoproteins ◽  
V3 Loop ◽  
Immunodeficiency Virus ◽  
Hiv 1

Human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (gp120 and gp41) elicit virus-neutralizing antibodies (VNAB) and also antibodies enhancing HIV-1 infection (EAB). Several epitopes eliciting VNAB have been defined, the principal virus-neutralizing determinant being assigned to the V3 loop of gp120. To provide a background for a rational design of anti-HIV vaccines, it also appears important to define domains eliciting EAB. This was accomplished by screening antisera against synthetic peptides covering almost the entire sequence of gp120/gp41 for their enhancing effects on HIV-1 infection of MT-2 cells, a continuous T cell line. Many (16/30) of the antisera significantly enhanced HIV-1 in the presence of human complement. Antibodies to complement receptor type 2 (CR2) abrogated the antibody-mediated enhancement of HIV-1 infection. Antisera to V3 hypervariable loops of 21 distinct HIV-1 isolates were also tested for their enhancing effects on HIV-1IIIB infection. 11 of these sera contained VNAB and 10 enhanced HIV-1IIIB infection. All antisera with virus-enhancing activity contained antibodies crossreactive with the V3 loop of HIV-1IIIB, and the virus-enhancing activity increased with increasing serological crossreactivity. These results suggest that immunization with antigens encompassing V3 loops may elicit EAB rather than protective antibodies if epitopes on the immunogen and the predominant HIV-1 isolate infecting a population are insufficiently matched, i.e., crossreactive serologically but not at the level of virus neutralization.

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